Information on EC 2.4.1.185 - flavanone 7-O-beta-glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.185
-
RECOMMENDED NAME
GeneOntology No.
flavanone 7-O-beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + a flavanone = UDP + a flavanone 7-O-beta-D-glucoside
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Flavonoid biosynthesis
-
-
hesperitin glycoside biosynthesis
-
-
naringenin glycoside biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:flavanone 7-O-beta-D-glucosyltransferase
Naringenin and hesperetin can act as acceptors. No action on flavones or flavonols.
CAS REGISTRY NUMBER
COMMENTARY hide
125752-73-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
Citrus mitis
-
-
-
Manually annotated by BRENDA team
Chinese wolfberry
UniProt
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucose + hesperetin
UDP + hesperetin 7-O-glucoside
show the reaction diagram
UDP-glucose + naringenin
UDP + naringenin 7-O-glucoside
show the reaction diagram
UDPglucose + 2',4',6',4-tetrahydroxychalcone
UDP + 2',4',6',4-tetrahydroxychalcone 4'-O-glucoside
show the reaction diagram
-
64% of activity with naringenin
-
?
UDPglucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-glucoside
show the reaction diagram
UDPglucose + apigenin
UDP + apigenin 7-O-glucoside
show the reaction diagram
-
peak I-III, 49%, 45% and 95% of activity with naringenin respectively
-
?
UDPglucose + kaempferol
UDP + kaempferol 7-O-glucoside
show the reaction diagram
UDPglucose + luteolin
UDP + luteolin 7-O-glucoside
show the reaction diagram
-
peak I, 52% of activity with naringenin
-
?
UDPglucose + quercetin
UDP + quercetin 7-O-glucoside
show the reaction diagram
additional information
?
-
does not use UDP-glucuronic acid, UDP-galactose, ADP-glucose, GDP-glucose, and TDP-glucose with esculetin as the glycosyl acceptor
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPglucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-glucoside
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP
-
10 mM, strong
ATP
-
10 mM, strong
Fe2+
enzyme activity is partially inhibited by 0.1 mM Fe2+ (43% residual activity)
Hg2+
enzyme activity is completely inhibited by 0.1 mM Hg2+
NADPH
-
10 mM, weak
TTP
-
10 mM, strong
UTP
-
10 mM, strong
additional information
Ca2+, Cd2+, Co2+, Cu2+, Mg2+, Mn2+, Ni2+, Sn2+, and Zn2+ (0.1 mM each) have negligible effects on catalytic activity
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13
2',4',6',4-tetrahydroxy chalcone
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-
0.11 - 0.124
hesperetin
0.008 - 0.08
naringenin
0.074 - 0.37
UDPglucose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.89
naringenin
Lycium barbarum
B6EWX3
at pH 7.2 and 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
97
naringenin
Lycium barbarum
B6EWX3
at pH 7.2 and 30°C
586
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00089 - 0.074
UDP
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
-
-
7.5 - 8
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hesperetin
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7.5
-
60% of maximal activity at pH 5.5 and pH 7.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
highest activity in leaves, more than in stem and roots
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
54400
-
gel filtration
54900
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gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10
at 20°C for 60 min
705189
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
stable below 40°C, pH 7.2, for 20 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 1% bovine serum albumin, 2-3 days, no loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, Sephadex G-100, hydroxyapatite, UDP-glucuronic acid agarose, Mono Q, Mono P
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ammonium sulfate, Superose 12, Mono Q, 3 peaks with 7-O-glucosyltransferase activity
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