Information on EC 2.4.1.181 - hydroxyanthraquinone glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.181
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RECOMMENDED NAME
GeneOntology No.
hydroxyanthraquinone glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + an hydroxyanthraquinone = UDP + a glucosyloxyanthraquinone
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:hydroxyanthraquinone O-glucosyltransferase
A range of anthraquinones and some flavones can act as acceptors; best substrates are emodin, anthrapurpurin, quinizarin, 2,6-dihydroanthraquinone and 1,8-dihydroxyanthraquinone.
CAS REGISTRY NUMBER
COMMENTARY hide
112198-78-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Cinchona succirubra
5 distinct glucosylating activities with different pI values and substrate specificities, named isoforms I-V
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 1,8-dihydroxyanthraquinone
UDP + 1-O-beta-D-glucopyranosyl-1,8-dihydroxyanthraquinone
show the reaction diagram
Cinchona succirubra
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-
-
-
?
UDP-alpha-D-glucose + 2,6-dihydroxyanthracene-9,10-dione
UDP + 6-hydroxy-9,10-dioxo-9,10-dihydroanthracen-2-yl beta-D-glucopyranoside
show the reaction diagram
Cinchona succirubra
-
-
-
-
?
UDP-alpha-D-glucose + anthrapurpurin
UDP + 2,7-dihydroxy-9,10-dioxo-9,10-dihydroanthracen-1-yl beta-D-glucopyranoside
show the reaction diagram
Cinchona succirubra
-
-
-
-
?
UDP-alpha-D-glucose + emodin
UDP + 7,8-dihydroxy-3-methyl-9,10-dioxo-9,10-dihydroanthracen-1-yl beta-D-glucopyranoside
show the reaction diagram
Cinchona succirubra
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-
-
-
?
UDP-alpha-D-glucose + quinizarin
UDP + 4-hydroxy-9,10-dioxo-9,10-dihydroanthracen-1-yl beta-D-glucopyranoside
show the reaction diagram
Cinchona succirubra
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-
-
-
?
UDP-glucose + a hydroxyanthraquinone
UDP + a glucosyloxyanthraquinone
show the reaction diagram
Cinchona succirubra
-
a range of anthraquinones and some flavones can act as acceptors
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + a hydroxyanthraquinone
UDP + a glucosyloxyanthraquinone
show the reaction diagram
Cinchona succirubra
-
a range of anthraquinones and some flavones can act as acceptors
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Cinchona succirubra
-
no cation requirement
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
Cinchona succirubra
-
60% inhibition at 1 mM
Cu2+
Cinchona succirubra
-
50% inhibition at 1 mM
iodoacetamide
Cinchona succirubra
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60% inhibition at 1 mM
iodoacetate
Cinchona succirubra
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30% inhibition at 1 mM
Mn2+
Cinchona succirubra
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45% inhibition at 10 mM
N-ethylmaleimide
Cinchona succirubra
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92% inhibition at 1 mM
p-chloromercuribenzoate
Cinchona succirubra
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47% inhibition at 1 mM, 10 mM dithiothreitol protects
phenylmercuric acetate
Cinchona succirubra
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80% inhibition at 1 mM, 10 mM dithiothreitol protects
Zn2+
Cinchona succirubra
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77% inhibition at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
Cinchona succirubra
-
63% activation at 10 mM
EDTA
Cinchona succirubra
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30% activation at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
1,8-dihydroxyanthraquinone
Cinchona succirubra
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isoform V
0.01
2,6-dihydroxyanthracene-9,10-dione
Cinchona succirubra
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isoform IV
0.01
anthrapurpurin
Cinchona succirubra
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isoform II
0.01
emodin
Cinchona succirubra
-
isoform I
0.01
quinizarin
Cinchona succirubra
-
isoform III
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Cinchona succirubra
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
Cinchona succirubra
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for isoforms I-V
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
Cinchona succirubra
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Cinchona succirubra
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
Cinchona succirubra
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gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
in absence of SH-group protectors, 50% loss of activity after 48 h, in presence of 14 mM 2-mercaptoethanol, 50% loss of activity after 2 weeks
Cinchona succirubra
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 25 mM histidine/HCl buffer, pH 7.0, 10% glycerol, 10 mM dithioerythritol, stable for 2 months
Cinchona succirubra
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
Cinchona succirubra
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