Information on EC 2.4.1.179 - lactosylceramide beta-1,3-galactosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.179
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RECOMMENDED NAME
GeneOntology No.
lactosylceramide beta-1,3-galactosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-galactose + beta-D-galactosyl-(1->4)-beta-D-glucosyl-R = UDP + beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-R
show the reaction diagram
lactose can also act as acceptor, but more slowly. Involved in the elongation of oligosaccharide chains, especially in glycolipids.; R may be an oligosaccharide or a glycolipid
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycosphingolipid biosynthesis - globo and isoglobo series
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SYSTEMATIC NAME
IUBMB Comments
UDP-galactose:D-galactosyl-(1->4)-beta-D-glucosyl-R 3-beta-galactosyltransferase
R may be an oligosaccharide or a glycolipid; lactose can also act as acceptor, but more slowly. Involved in the elongation of oligosaccharide chains, especially in glycolipids.
CAS REGISTRY NUMBER
COMMENTARY hide
106769-64-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
female tammar wallaby
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Manually annotated by BRENDA team
no activity in Mus musculus
lactating mammary gland
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-galactose + asialo-fetuin
UDP + beta-D-galactosyl-1,3-asialo-fetuin
show the reaction diagram
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-
-
-
?
UDP-galactose + asialo-glycophorin A
UDP + beta-D-galactosyl-1,3-asialo-glycophorin A
show the reaction diagram
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-
-
-
?
UDP-galactose + asialo-orosomucoid
UDP + beta-D-galactosyl-1,3-asialo-orosomucoid
show the reaction diagram
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-
-
-
?
UDP-galactose + beta-D-galactose
UDP + beta-D-galactosyl-1,3-beta-D-galactose
show the reaction diagram
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-
-
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?
UDP-galactose + beta-D-galactosyl-1,3-N-acetyl-D-galactosamine
UDP + beta-D-galactosyl-1,3-beta-D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
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-
-
-
?
UDP-galactose + beta-D-galactosyl-1,6-N-acetylglucosylamine
UDP + beta-D-galactosyl-1,3-beta-D-galactosyl-1,6-N-acetylglucosylamine
show the reaction diagram
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-
-
-
?
UDP-galactose + beta-D-galactosyl-O-4-nitrophenyl
UDP + beta-D-galactosyl-1,3-beta-D-galactose-O-4-nitrophenyl
show the reaction diagram
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-
-
-
?
UDP-galactose + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
show the reaction diagram
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-
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?
UDP-galactose + D-galactosyl-1,4-beta-D-glucose
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
show the reaction diagram
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid
show the reaction diagram
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-R
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-R
show the reaction diagram
UDP-galactose + ganglioside GM2
UDP + beta-D-galactosyl-1,3-ganglioside GM2
show the reaction diagram
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?
UDP-galactose + lacto-N-tetraose
UDP + beta-D-galactosyl-1,3-lacto-N-tetraose
show the reaction diagram
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?
UDP-galactose + lactosylceramide
?
show the reaction diagram
UDP-galactose + lactotriaosylceramide
UDP + lactotetraosylceramide
show the reaction diagram
UDP-galactose + lactulose
UDP + beta-D-galactosyl-1,3-lactulose
show the reaction diagram
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?
UDP-galactose + melibiose
UDP + beta-D-galactosyl-1,3-melibiose
show the reaction diagram
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?
UDP-galactose + methyl-alpha-galactoside
UDP + beta-D-galactosyl-1,3-methyl-alpha-galactoside
show the reaction diagram
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?
UDP-galactose + methyl-beta-galactoside
UDP + beta-D-galactosyl-1,3-methyl-beta-galactoside
show the reaction diagram
UDP-galactose + N-acetylgalactosamine
UDP + beta-D-galactosyl-1,3-N-acetylgalactosamine
show the reaction diagram
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?
UDP-galactose + N-acetyllactosamine
UDP + beta-D-galactosyl-1,3-N-acetyllactosamine
show the reaction diagram
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?
UDP-galactose + p-nitrophenyl-alpha-N-acetylgalactosylamine
UDP + beta-D-galactosyl-1,3-N-acetyl-alpha-galactosylaminyl-4-nitrophenyl
show the reaction diagram
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?
UDP-galactose + stachyose
UDP + beta-D-galactosyl-1,3-stachyose
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid
UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid
show the reaction diagram
additional information
?
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participates in the synthesis of glycosphingolipids
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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activation, can replace Mn2+ with 26% efficiency
Cd2+
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Co2+
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activation, can replace Mn2+ with 51% efficiency
Cu2+
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activation, can replace Mn2+ with 15% efficiency
Mg2+
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activation, can replace Mn2+ with 13% efficiency
Ni2+
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activation, can replace Mn2+ with 16% efficiency
additional information
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no activation by Zn2+ or Cd2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Bovine liver non-specific transfer proteins
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activation
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CHAPSO
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5 to 7fold less effective compared to Triton CF-54
deoxycholate
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5 to 7fold less effective compared to Triton CF-54
glycerol
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requirement, above 20% w/v
phosphatidylethanolamine
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activation, less efficient than Triton CF-54
taurodeoxycholate
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5 to 7fold less effective compared to Triton CF-54
Triton CF-54
Triton X-100
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activation, can replace Triton CF-54 with 75% less efficiency
additional information
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little or no activation by phosphatidylcholine, phosphatidylglycerol, phosphatidylserine, phosphatidylinositol or cardiolipin, Brij-58, Empigen BB or detergent G-3634-A
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
46 - 242
lactose
2.5
lactosylceramide
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0.013
lactotriaosylceramide
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0.048 - 0.17
UDP-galactose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00013
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recombinant in HCT-15 cells
0.00077
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0.02 - 0.032
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from day 90 to day 225 post partum
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8
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assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
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about 75% of maximal activity at pH 5 and about half-maximal activity at pH 8.0
5.3 - 7.5
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about 80% of maximal activity at pH 5.3 and about 55% of maximal activity at pH 7.5
5.8 - 8.2
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about half-maximal activity at pH 5.8 and pH 8.2, cacodylate or Tris-HCl buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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cDNA for cloning taken from brain tissue
Manually annotated by BRENDA team
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colonic adenocarcino cell line
Manually annotated by BRENDA team
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colonic adenocarcino cell line
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36
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t1/2: 20 min
39
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30 min, complete inactivation, in the presence of Mn2+ 11% loss of activity within 60 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol, 20% w/v and above, stabilizes
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Mn2+ enhances thermal stability
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, in 20% glycerol, several weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from Colo 205 cell line; partial
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partial
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in CHO-K1 cells as haemagglutinin and yellow fluorescent protein fusion protein
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functional expression in HCT-15 cells
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