Information on EC 2.4.1.174 - glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.174
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RECOMMENDED NAME
GeneOntology No.
glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
chondroitin biosynthesis
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Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-galactosamine:D-glucuronyl-(1->3)-beta-D-galactosyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase
Requires Mn2+. Involved in the biosynthesis of chondroitin sulfate. Key enzyme activity for the initiation of chondroitin and dermatan sulfates, transferring GalNAc to the GlcA-Gal-Gal-Xyl-Ser core.
CAS REGISTRY NUMBER
COMMENTARY hide
96189-39-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
calf
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Manually annotated by BRENDA team
gene csgalnact2
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the CSGALNACT1 and CSGALNACT2 isozymes enzymes both have a GT7 catalytic domain with beta(1->4)-N-acetylgalactosaminyl transferase; the CSGALNACT1 and CSGALNACT2 isozymes enzymes both have a GT7 catalytic domain with beta(1->4)-N-acetylgalactosaminyl transferase
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan
UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan
show the reaction diagram
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactose
UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactose
show the reaction diagram
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minimum requirement for acceptor structure
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-
?
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactose
UDP + N-acetyl-D-galactosaminyl-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactose
show the reaction diagram
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best substrate
-
?
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-(Gly)Ser-(Gly-Glu)
UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-(Gly)Ser-(Gly-Glu)
show the reaction diagram
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-
-
?
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser
UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser
show the reaction diagram
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-
-
?
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser-peptide
UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser-peptide
show the reaction diagram
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-
-
?
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl
UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl
show the reaction diagram
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
show the reaction diagram
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-
-
-
?
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1-O-C2H4-NH-benzyloxycarbonyl
UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1-O-C2H4-NH-benzyloxycarbonyl
show the reaction diagram
UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)
UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)
show the reaction diagram
UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin
UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin
show the reaction diagram
UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides
UDP + N-acetyl-D-galactosaminyl-1,4-beta-D-glucuronylgalactosyl glycosides
show the reaction diagram
UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides
UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan
UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan
show the reaction diagram
UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides
UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides
show the reaction diagram
additional information
?
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in vitro chondroitin polymerization does not occur on the non-reducing terminal GalNAc-linkage pentasaccharide structure
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
brefeldin A
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additional information
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heparan sulfate-synthesis enzymes inhibits chondroitin sulfate as extracellular inhibitor of axon growth
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Manually annotated by BRENDA team
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low content
Manually annotated by BRENDA team
pectoral; pectoral and median
Manually annotated by BRENDA team
melanoma cell line
Manually annotated by BRENDA team
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very high content
Manually annotated by BRENDA team
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expression of Chsy1 is up-regulated in skeletal elements
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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chondrosarcoma cell line
Manually annotated by BRENDA team
thyroid cancer cell line
Manually annotated by BRENDA team
low content
Manually annotated by BRENDA team
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low content
Manually annotated by BRENDA team
myeloma cell line
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
confocal microscopy analysis of isozyme localization
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70000
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x * 70000, about, recombinant His-tagged soluble forms of wild-type ChGn-1 and S126LChGn-1 mutant enzymes, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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1 h, 10% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially solubilized with a buffer containing 2% Triton X-100, 20% glycerol and phospholipase A2
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recombinant His-tagged soluble forms of wild-type ChGn-1 and S126LChGn-1 mutant from COS-1 cellsby nickel affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CSGalNAcT1 expression in C57BL/6 ES cells
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CSS1 quantitative expression analysis by realtime RT-PCR, overview
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DNA sequence determination and analysis, expression of a truncated form, consisting of the putative catalytic domain, in Spodoptera frugiperda Sf21 insect cells as a soluble FLAG-tagged protein
DNA sequence determination, genomic organization and chromosome localization, functional expression as soluble protein fused to protein A IgG-binding domain in COS-1 cells, secretion into the medium
expression of ChGn-1 in mutant sog9 L cells
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gene ChGn1, expression of His-tagged wild-type ChGn-1 and soluble mutant enzymes in COS-1 cells, secretion to the medium
gene Csgalnact1
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gene csgalnact1, DNA and amino acid sequence determination and analysis, phylogenetic analysis; gene csgalnact2, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene CSGALNACT1, genotyping and polymorphisms, recombinant expression of His-tagged soluble forms of wild-type ChGn-1 and S126LChGn-1 mutant in COS-1 cells, the enzymes are secreted
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gene CSGALNACT1, quantitative real-time PCR enzyme expression analysis
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gene CSGALNACT1, quantitative real-time RT-PCR enzyme expression analysis; gene CSGALNACT2, quantitative real-time RT-PCR enzyme expression analysis
gene CSGalNAcT2, cloning from DF-1 cells, overexpression in DF1 cells, semiquantitative real-time RT-PCR enzyme expression analysis, interaction analysis of enzyme CSGalNAcT2 with IBDV viral capsid protein VP2using the yeast two-hybridization assay
LTC cells from rat chondrosarcoma are transfected with chondroitin sulfate N-acetylgalactosaminyltransferase-1 expression plasmid (about 80fold increase of chondroitin sulfate N-acetylgalactosaminyltransferase-1 RNA), metabolic albeling with [35S]sulfate show up to a 2.2fold increase in chondroitin sulfate levels in these cells, but the mRNA level of aggrecan core protein does not change. Aggrecan obained from chondroitin sulfate N-acetylgalactosaminyltransferase-1 overexpressing cells contain a larger amount of chondroitin sulfate, but the chondroitin sulfate chain length is similar to mock-transfected cells.
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Myc-tagged CSGalNAcT-1 expression in HeLa cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
CSGalNAcT2 gene expression is upregulated during infectious bursal disease virus, IBDV, infection
the enzyme is downregulated in Kashin-Beck disease (KBD), an endemic degenerative osteoarthritis, and in primary osteoarthritis, both associated with extracellular matrix degradation
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H234R
naturally occuring mutation in exon 5 of gene ChGn1, the mutant shows neuropathy, Bellís palsy and/or hereditary motor and sensory neuropathy, the mutant enzyme is inactive
M509R
naturally occuring mutation in exon 10 of gene ChGn1, the mutant shows neuropathy, Bellís palsy and/or hereditary motor and sensory neuropathy, the mutant enzyme is inactive
S126L
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naturally occuring inactive ChGN1 mutant
additional information