Information on EC 2.4.1.172 - salicyl-alcohol beta-D-glucosyltransferase

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The expected taxonomic range for this enzyme is: Gardenia jasminoides

EC NUMBER
COMMENTARY hide
2.4.1.172
-
RECOMMENDED NAME
GeneOntology No.
salicyl-alcohol beta-D-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + salicyl alcohol = UDP + salicin
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
salicin biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:salicyl-alcohol beta-D-glucosyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
89400-32-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDPglucose + salicyl alcohol
UDP + salicin
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPglucose + salicyl alcohol
UDP + salicin
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
-
2 mM, 80% inhibition
Mn2+
-
2 mM, 20% inhibition
N-ethylmaleimide
-
10 mM, 35% inhibition
p-chloromercuribenzoate
-
10 mM, 25% inhibition
Zn2+
-
2 mM, 60% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.11 - 0.53
salicyl alcohol
0.031 - 0.64
UDPglucose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10.5
-
45% of maximal activity at pH 6.0, 10% of maximal activity at pH 10.5
7.5 - 10.2
-
half maximal activity at pH 7.5 and 10.2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 55
-
20% of maximal activity at 30C, 35% of maximal activity at 55C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51000
-
gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
-
stable
488805
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
5 min, 10% remaining activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 3 weeks, 50% loss of activity
-
4C, 2 weeks, 40% loss of activity, or -20C, 2 weeks, 40% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE