Information on EC 2.4.1.165 - N-acetylneuraminylgalactosylglucosylceramide beta-1,4-N-acetylgalactosaminyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.165
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RECOMMENDED NAME
GeneOntology No.
N-acetylneuraminylgalactosylglucosylceramide beta-1,4-N-acetylgalactosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-galactosamine + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-[alpha-N-acetylneuraminyl-(2->3)]-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-galactosamine:N-acetylneuraminyl-(2->3)-alpha-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide 4-beta-N-acetylgalactosaminyltransferase
Requires Mn2+. Only substances containing sialic acid residues can act as acceptors; bovine fetuin is the best acceptor tested.
CAS REGISTRY NUMBER
COMMENTARY hide
109136-50-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + alpha(2-3)-sialylated glycoprotein
UDP + GalNAc-beta(1-4)(Neu5Ac(2-3))Gal-beta-1-protein
show the reaction diagram
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?
UDP-N-acetyl-D-galactosamine + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-[alpha-N-acetylneuraminyl-(2->3)]-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + alpha1-acid glycoprotein
UDP + N-acetylgalactosaminyl-alpha1-acid glycoprotein
show the reaction diagram
UDP-N-acetyl-D-galactosamine + asialofetuin
UDP + N-acetylgalactosaminylasialofetuin
show the reaction diagram
UDP-N-acetyl-D-galactosamine + di-sialyl-lacto-N-tetraose
UDP + N-acetylgalactosaminyl-di-sialyl-lacto-N-tetraose
show the reaction diagram
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?
UDP-N-acetyl-D-galactosamine + fetuin
UDP + ?
show the reaction diagram
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?
UDP-N-acetyl-D-galactosamine + fetuin
UDP + N-acetylgalactosaminylfetuin
show the reaction diagram
UDP-N-acetyl-D-galactosamine + glycophorin
UDP + N-acetylgalactosaminyl glycophorin
show the reaction diagram
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good acceptor
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?
UDP-N-acetyl-D-galactosamine + glycophorin A
UDP + N-acetylgalactosaminyl glycophorin A
show the reaction diagram
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weak activity
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?
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin
UDP + N-acetylgalactosaminyl human chorionic gonadotropin
show the reaction diagram
UDP-N-acetyl-D-galactosamine + Neu5Ac-alpha(2-3)Gal-beta(1-3)GlcNAc-beta-1-O-benzyl
UDP + ?
show the reaction diagram
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?
UDP-N-acetyl-D-galactosamine + NeuAcalpha(2-3)Galbeta(1-4)Glc
UDP + GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc
show the reaction diagram
UDP-N-acetyl-D-galactosamine + NeuAcalpha(2-3)Galbeta(1-4)GlcNAc
UDP + GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-[alpha-N-acetylneuraminyl-(2->3)]-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + NeuAcalpha(2-3)Galbeta(1-4)Glc
UDP + GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc
show the reaction diagram
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responsible for the final step in the formation of the Sda/Cad antigenic epitopes, Sd(alpha) beta4GalNAcT-II mRNA transcript is drastically reduced in oncogenetic processes in gastrointestinal tissues
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?
additional information
?
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Q8NHY0
involved in formation of blood group Sda/CAD antigen
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
n-octylglucoside
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20-30% enhancement of activity with the proteins that behave as good acceptors: fetuin, alpha1-glycoprotein and human chorionic gonadotropin. 3fold enhancement of activity of the poor acceptor glycophorin
Triton X-100
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.222 - 0.94
fetuin
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1.1
N-acetylneuraminyl-2,3-alpha-D-galactosyl-1,4-beta-D-glucosylceramide
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0.38
Neu5Ac-alpha(2-3)Gal-beta(1-3)GlcNAc-beta-1-O-benzyl
pH 6.2, 37°C
0.11
UDP-N-acetyl-D-galactosamine
pH 6.2, 37°C
0.055 - 0.064
UDP-N-acetylgalactosamine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8 - 9.5
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about half-maximal activity at pH 5.8 and 9.5
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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group O
Manually annotated by BRENDA team
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high levels of enzyme in normal colonic mucosa
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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Manually annotated by BRENDA team
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large amounts of the enzyme is released in soluble form, particularly when CaCo-2 cells are maintained in culture for more than 3 weeks in order to ensure an higher degree of enterocyte differentiation
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Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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protein has a typical type II glycosyltransferase organization and a 67 amino acid cytoplasmic tail
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no glycoprotein
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contains no potential N-glycosylation sites
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20 C, microsomal fraction, 1 year without significant loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
transgenic mice overexpress the enzyme in the skeletal muscle of mdx animals
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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the enzyme is a marker of the colonic cell maturation
medicine