Information on EC 2.4.1.164 - galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide beta-1,6-N-acetylglucosaminyltransferase

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The expected taxonomic range for this enzyme is: Mus musculus

EC NUMBER
COMMENTARY hide
2.4.1.164
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RECOMMENDED NAME
GeneOntology No.
galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide beta-1,6-N-acetylglucosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-D-glucosamine + D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-D-glucosaminyl-(1->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide 6-beta-N-acetylglucosaminyltransferase
Requires Mn2+.
CAS REGISTRY NUMBER
COMMENTARY hide
85638-40-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
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i.e. neolactotetraosylceramide, GlcNAcT-3, synthesis in vitro of Ii core glycosphingolipids
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?
UDP-N-acetyl-D-glucosamine + lactosylceramide
?
show the reaction diagram
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15% of the activity with neolactotetraosylceramide
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?
UDP-N-acetyl-D-glucosamine + sialic acid-depleted alpha1-acid glycoprotein
?
show the reaction diagram
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30% of the activity with neolactotetraosylceramide
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?
additional information
?
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biosynthesis of Ii core glycosphingolipids
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
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biosynthesis of Ii core glycosphingolipids
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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activates, 48% as effective as Mn2+
Co2+
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activates, 52% as effective as Mn2+
Mn2+
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required, Km: 1.25 mM, Mn2+ cannot be replaced by Mg2+, Zn2+, Cu2+ or Cd2+
additional information
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not activated by Mg2+, Zn2+, Cu2+ or Cd2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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complete inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Detergent
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activates, reaction rate is optimal at detergent:protein ratio of 1.6 in Triton CF-54 or X-100, 1:3 in Nonidet P-40
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Nonidet P-40
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reaction rate is optimal at detergent:protein ratio of 1:3
Triton CF-54
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reaction rate is optimal at detergent:protein ratio of 1:6
Triton X-100
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reaction rate is optimal at detergent:protein ratio of 1:6
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.09
neolactotetraosylceramide
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0.33 - 2.5
UDP-N-acetyl-D-glucosamine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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lymphoma cell line
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE