Information on EC 2.4.1.155 - alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.155
-
RECOMMENDED NAME
GeneOntology No.
alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-D-glucosamine + 6-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mannosyl-glycoprotein N-acetylglucosaminyltransferases
-
-
Metabolic pathways
-
-
N-Glycan biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:6-[2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl]-glycoprotein 6-beta-N-acetyl-D-glucosaminyltransferase
R represents the remainder of the N-linked oligosaccharide in the glycoprotein acceptor (click here for diagram).
CAS REGISTRY NUMBER
COMMENTARY hide
83588-90-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene MGAT5
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
-
beta1,6-N-acetylglucosaminyltransferase V (GnT-V) is encoded by ets-1, a transcriptional factor which also regulates several enzymes associated with cell invasion and metastasis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-1-antitrypsin precursor + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
angiotensinogen preproprotein + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
beta-N-acetylhexosaminidase A + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
cathepsin D preproprotein + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
cathepsin X precursor + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
DDR1 variant protein + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
dipeptidyl peptidase 7 preproprotein + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
discoidin receptor tyrosine kinase + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
dystroglycan 1 precursor + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
galectin 3 binding protein + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
granulin isoform 1 precursor + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
heat shock protein 70 kDa protein 8 isoform 1 + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
heat shock protein gp96 precursor + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
heparan sulfate proteoglycan perlecan + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
hexosaminidase B preproprotein + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
human Fc binding protein + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
Ig kappa chain V-III + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
laminin gamma1 precursor + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
legumain preproprotein + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
lysosomal-associated membrane protein-1 + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
LAMP-1
-
-
?
lysosome-associated membrane protein-1 + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
LAMP-1
-
-
?
met proto-oncogene + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
N-acetylgalactosamine-6-sulfatase + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
N-acetylglucosamine-6-sulfatase + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
prosaposin + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
protective protein for beta-galactosidase + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
protein tyrosine phosphatase kappa + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
protein-tyrosine kinase-related receptor PTK7 + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
ribonuclease T2 precursor + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
TDP-glucose + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
TDP + ?
show the reaction diagram
-
-
-
-
?
tissue inhibitor of metalloproteinase-1 + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
tumor-associated calcium signal transducer 1 + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
tyrosinase + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
-
-
-
?
tyrosinase-related protein-1 + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
TYRP-1
-
-
?
tyrosinase-related protein-2 + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
TYRP-2
-
-
?
UDP-GlcNAc + GlcNAc-beta(1-2)Man-alpha(1-3)(GlcNAc-beta(1-2)Man-alpha(1-4))Man-beta(1-4)GlcNAc-2-amino pyridine
?
show the reaction diagram
-
-
-
-
?
UDP-glucose + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + 6-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
UDP + 6-(2,6-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + Ac-Val-Glu-Pro-(GlcNAcbeta(1-2)Manalpha1-O-)Thr-Ala-Val-NH2
UDP + Ac-Val-Glu-Pro-(GlcNAcbeta(1-2)[GlcNAcbeta(1-6)]Manalpha1-O-)Thr-Ala-Val-NH2
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + epidermal growth factor receptor
UDP + N-acetylglucosamine-beta-1,6-mannose-alpha-1,3-branch of epidermal growth factor receptor
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-2)Manalpha(1-2)Glc-O-octyl
UDP + GlcNAcbeta(1-2)[GlcNAcbeta(1-6)]Manalpha(1-2)Glc-O-octyl
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-2)Manalpha(1-3)[GlcNAcbeta(1-2)Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc
UDP + GlcNAcbeta(1-2)[GlcNAcbeta(1-6)]Manalpha(1-3)[GlcNACbeta(1-2)Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-2)Manalpha1-O-benzyl
UDP + GlcNAcbeta(1-2)[GlcNAcbeta(1-6)]Manalpha1-O-benzyl
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-4-deoxy-mannosyl-1,6-beta-glucosyl-O-(CH2)7-CH3
UDP + N-acetyl-D-glucosaminyl-1,6-(N-acetyl-D-glucosaminyl-1,2)-alpha-4-deoxy-mannosyl-1,6-beta-D-mannosyl-O-(CH2)7-CH3
show the reaction diagram
-
good substrate
-
-
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-L-Asn
UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-L-Asn
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-glucosyl-O-R
UDP + N-acetyl-D-glucosaminyl-1,6-(N-acetyl-D-glucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-glucosyl-O-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-mannosyl-O-(CH2)8COOCH3
UDP + N-acetyl-D-glucosaminyl-1,6-(N-acetyl-D-glucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-mannosyl-O-(CH2)8COOCH3
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-mannosyl-O-R
UDP + N-acetyl-D-glucosaminyl-1,6-(N-acetyl-D-glucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-mannosyl-O-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-glucosaminyl-O-(CH2)7-CH3
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + octyl 6-O-[2-O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-alpha-D-mannopyranosyl]-beta-D-glucopyranoside
UDP + ?
show the reaction diagram
UDP-N-acetyl-D-glucosamine + [GlcNAcbeta(1-2)][GlcNAcbeta(1-6)]Manalpha(1-3)[GlcNAcbeta(1-2)Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc
UDP + [GlcNAcbeta(1-2)][GlcNAcbeta(1-6)]Manalpha(1-3)[[GlcNAcbeta(1-2)][GlcNAcbeta(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc
show the reaction diagram
-
-
-
-
?
Zn-alpha-2-glycoprotein + UDP-N-acetyl-D-glucosamine
?
show the reaction diagram
-
identified target protein for GnT-V in human colon cancer cell WiDr
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + 6-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
UDP + 6-(2,6-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + epidermal growth factor receptor
UDP + N-acetylglucosamine-beta-1,6-mannose-alpha-1,3-branch of epidermal growth factor receptor
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-L-Asn
UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-L-Asn
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MnCl2
-
up to 3fold stimulation
NaCl
-
activation, 0.2 M
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP-N-acetylglucosamine
-
20 mM
antisense GnT-V
-
-
-
Ba2+
-
30% of maximal activity
beta1,4-N-acetylglucosaminyltransferase-III
-
competitive inhibitor
-
beta1,6-N-acetylglucosaminyltransferase III
-
GnT-III, competitive inhibitor
-
Ca2+
-
20% of maximal activity; 35% of maximal activity
CDP-N-acetylglucosamine
-
20 mM
Cu2+
-
30% of maximal activity
EDTA
-
10% of maximal activity in absence of any cation and in presence in EDTA
Fe2+
-
15% of maximal activity
Fe3+
-
1 mM inhibits
GDP-N-acetylglucosamine
-
20 mM
Mg2+
-
50% of maximal activity
N-Acetyl-D-glucosaminyl-1,2-alpha-4-O-methyl-mannosyl-1,6-beta-D-glucosyl-O-(CH2)7-CH3
-
-
N-Acetyl-D-glucosaminyl-1,2-alpha-6-deoxy-mannosyl-1,6-beta-D-glucosyl-O-(CH2)7-CH3
N-Acetyl-D-glucosaminyl-1,2-alpha-6-deoxy-mannosyl-1,6-beta-D-glucosyl-O-(CH2)8-COOCH3
-
-
N-bromosuccinimide
-
1 mM inhibits
NaCl
-
more than 0.2 M, decrease in enzyme acitivty; more than 50 mM, decrease in enzyme acitivty
Ni2+
-
15% of maximal activity
PC-MGAT5si
-
a MGAT5 knock-down vector is used for animal studies in mice
-
siRNA
-
TDP-N-acetylglucosamine
-
20 mM
Zn2+
-
10% of maximal activity
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glycerol
-
activation and stabilization, 20%
Immunoglobulin G
-
activation and stabilization, 0.5 mg/ml
-
Triton X-100
-
activation, 1.0-1.5%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05 - 1.11
Ac-Val-Glu-Pro-(GlcNAcbeta(1-2)Manalpha1-O-)Thr-Ala-Val-NH2
1.28 - 2.4
GlcNAc-beta(1-2)Man-alpha(1-3)(GlcNAc-beta(1-2)Man-alpha(1-4))Man-beta(1-4)GlcNAc-2-amino pyridine
0.53 - 1.82
GlcNAcbeta(1-2)Manalpha(1-2)Glc-O-octyl
0.61 - 0.88
GlcNAcbeta(1-2)Manalpha1-O-benzyl
0.25
N-acetyl-D-glucosamine
-
-
0.074
N-acetyl-D-glucosaminyl-1,2-alpha-4-deoxy-mannosyl-1,6-beta-D-glucosyl-O-(CH2)7-CH3
-
-
-
0.13 - 0.23
N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
0.023 - 0.036
N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-glucosyl-O-(CH2)7-CH3
-
0.18
N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-mannosyl-O-(CH2)-COOCH3
-
-
-
0.087
N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-mannosyl-O-(CH2)8COOCH3
-
-
0.133
pyridylaminated biantennary acceptor substrates
-
-
-
2.9
TDP-glucose
-
-
4.5 - 5.8
UDP-GlcNAc
2.9
UDP-glucose
-
-
0.56 - 1.62
UDP-N-acetyl-D-glucosamine
1.1 - 11
UDP-N-acetylglucosamine
0.66 - 1.4
UDP-N-acetylgucosamine
additional information
additional information
-
kinetic study
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.014
N-Acetyl-D-glucosaminyl-1,2-alpha-4-O-methyl-mannosyl-1,6-beta-D-glucosyl-O-(CH2)7-CH3
-
-
0.06 - 0.077
N-Acetyl-D-glucosaminyl-1,2-alpha-6-deoxy-mannosyl-1,6-beta-D-glucosyl-O-(CH2)7-CH3
0.141
N-Acetyl-D-glucosaminyl-1,2-alpha-6-deoxy-mannosyl-1,6-beta-D-glucosyl-O-(CH2)8-COOCH3
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00000003
-
heart, wild-type mice
0.0000006
-
brain, wild-type mice
0.000001
-
spleen, wild-type mice
0.0000012
-
liver, wild-type mice
0.00000133
-
kidney, wild-type mice
0.00000275
-
intestine, wild-type mice
0.0015 - 0.031
-
-
0.0036
-
BVVDELTA73 mutant
0.004
-
BVVDELTA188 mutant
0.03 - 0.06
-
Q39-740 mutant
0.139
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 6.7
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
42
-
2fold increase as compared to 37°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
mouse mammary tumor cell line
Manually annotated by BRENDA team
-
melanotic hybrid cell
Manually annotated by BRENDA team
-
melanotic hybrid cell
Manually annotated by BRENDA team
-
observed in 6/28 cases
Manually annotated by BRENDA team
-
native BHK-21/c13 cells, cells transformed with Rous sarcoma virus, i.e. RS-BHK-cells, and lectin-resistant cell-lines LP 1.6 and 3.3
Manually annotated by BRENDA team
-
of the embryo
Manually annotated by BRENDA team
-
endometrial endometrioid adenocarcinoma cell
Manually annotated by BRENDA team
-
oberserved in 10/23 cases
Manually annotated by BRENDA team
immunohistochemic analysis of 313 clinical samples, overview
Manually annotated by BRENDA team
-
testicular germ cell
Manually annotated by BRENDA team
-
of the adult
Manually annotated by BRENDA team
-
higher expression of GnT-V is correlated with a favorable prognosis for neuroblastoma patients. GnT-V may cause these tumors to regress by increasing their susceptibility to apoptosis
Manually annotated by BRENDA team
-
observed in 66/68 cases
Manually annotated by BRENDA team
-
of the embryo
Manually annotated by BRENDA team
non-tumor tissue shows strong expression of MGAT5, immunohistochemic analysis
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65000
-
native PAGE
67000
-
SDS-PAGE
69000
-
SDS-PAGE, 2 bands: 69000 and 75000
70000
-
SDS-PAGE, BVVdelta188
73000
-
HPLC-gel filtration
75000
-
SDS-PAGE, 2 bands: 69000 and 75000
84570
-
determined by SDS-PAGE and Western Blot analysis
85000
-
SDS-PAGE, BVVdelta73
100000
-
determined by SDS-PAGE and Western Blot analysis
110000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 73000, SDS-PAGE
additional information
-
rat kidney enzyme appears as doublet of MW 69000 and 75000 on SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
proteolytic modification
-
Golgi-resident GnT-V is cleaved mainly in the transmembrane region by gamma-secretase, followed by its secretion
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 10.5
-
stable for 24 h at 4°C
638555
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
stable for 2 months
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol, 20%, stabilizes and enhances activity
-
immunoglobulin G, 0.5 mg/ml, stabilizes and enhances activity
-
NaCl, 0.2 M, stabilizes and enhances activity
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C stable for 2 months
-
4°C, several months in 20% glycerol
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
GnT-Vdelta73, GnT-Vdelta233, GnT-Vdelta436 and GnT-Vdelta188
-
nickel column chromatography
-
partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a recombinant vector MGAT5-pCXNneo is constructed for transfection of WiDr cells
-
fusion with signal peptide from baculoviral protein to permit secretion into medium, BVVdelta73 and BVV delta188
-
gene MGAT5, overexpression in CHO cells and Lec4 cells from Cricetulus griseus
gene MGAT5, quantitative real-time PCR enzyme expression analysis
-
gene MGAT5, real-time RT-PCR enzyme expression analysis, establishing of human melanoma cell line WM-266-4 with induced stable overexpression of GnT-V, the cells show ncreased amounts of bisected and beta1-6 branched N-glycans on melanoma cell adhesion molecule MCAM/MUC18, but do not display significant differences in viability and capabilities to migrate through an endothelial layer, coexpression with MGAT3 gene, EC 2.4.1.144
-
gene MGAT5, recombinant tet-inducible expression of Flag-tagged GlcNAcT-V in HeLa and HEK293 cells. Tet-induced Mgat5 HEK293 cells display a 2fold increase in tri-antennary and a 2fold increase in tetraantennary N-glycans
-
Huh7 and HepG2 cells are transiently transfected by both Mgat5 expression vector plasmid and empty vector plasmid as a control
-
into the pcDNA3 vector for transfection of rat pheochromocytoma cells, PC12
-
into the pGEM-Teasy vector and subcloned into the pcDNA3 expression vector
-
MKN45 cells are transfected with GnT-III, GnT-V, or both, respectively
-
transfection in HEK-293 cells
-
transfection into 7721 cells
-
wild type enzyme and several N- and C-terminal deletions, fusion to protein A from Staphylococcus aurus
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
decreased expression after exogenous addition alltrans-retinoic acid
-
during interferon-gamma-induced inflammation, the level of GnT-V in THP-1 monocytes are significantly decreased
-
high GnT-V expression correlated with FIGO surgical stage, strong immunostaining in mucinous adendocarcinoma
-
increased expression after anti-CD3 stimulation
-
overexpressed in malignant tumors
-
selective knockdown of GnT-Va expression, inhibition of ligand-induced downregulation of the epidermal growth factor receptor and intracellular signaling by inhibiting receptor endocytosis
-
the expression of GnT-V is quite low in normal liver tissue but is increased under conditions of chronic hepatitis and liver regeneration
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A592G
-
increased specific activity
L188R
-
site-directed mutagenesis, inactive mutant
T555R
-
increased specific activity
V375L
-
increased specific activity
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
-
transfection of sense cDNA of enzyme results in increase in the N-acetylglucosamine-beta1,6-mannose-alpha1,3-branch of epidermal growth factor receptor. Transfection with antisense CDNA of enzyme rsults in reverse reaction. Altered expression of enzyme will change the glycan structure and function of epidermal growth factor receptor, which may modify downstream signal transduction
diagnostics
the enzyme is useful as diagnostic marker for gastric adenocarcinomas: low intratumoral MGAT5 density is associated with poor differentiation, N classification, TNM stage, and Kiel stage, and is an independent prognosticator for poor overall survival. The combination of intratumoral MGAT5 expression and TNM or Kiel staging systems has a better predictive power for overall survival. Applying the prognostic value of intratumoral MGAT5 density to TNM stage III+IV and Kiel stage IIIB+IV groups shows a better risk stratification for overall survival in patients with late-stage gastric cancer, overview
medicine
pharmacology
-
the work is a step toward intriguing innovative therapeutic strategies for trials currently in consider with glycosyltransferase in neurodegenerative disorders
additional information
-
an elevation of enzyme Gnt-V activity leads to radiosensitivity and migration of small cell lung cancer cells by inducing epithelial-mesenchymal transition, EMT, thereby highlighting Gnt-V as a potential therapeutic target for the prevention of EMT-associated tumour radioresistance and migration