Information on EC 2.4.1.15 - alpha,alpha-trehalose-phosphate synthase (UDP-forming)

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
2.4.1.15
-
RECOMMENDED NAME
GeneOntology No.
alpha,alpha-trehalose-phosphate synthase (UDP-forming)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Starch and sucrose metabolism
-
-
trehalose biosynthesis I
-
-
metabolism of disaccharids
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:D-glucose-6-phosphate 1-alpha-D-glucosyltransferase
See also EC 2.4.1.36 [alpha,alpha-trehalose-phosphate synthase (GDP-forming)].
CAS REGISTRY NUMBER
COMMENTARY hide
9030-07-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain SN223/29
UniProt
Manually annotated by BRENDA team
strain SN223/29
UniProt
Manually annotated by BRENDA team
gene TPS
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
isolated from the alpine permafrost, CGMCC 1.8987, gene otsA
UniProt
Manually annotated by BRENDA team
gene otsA
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain H99
SwissProt
Manually annotated by BRENDA team
gene cftps; gene cftps
UniProt
Manually annotated by BRENDA team
bifunctional trehalose 6-phospate synthase and trehalose 6-phospate phosphatase enzyme
-
-
Manually annotated by BRENDA team
gene TPS
UniProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
gene tps1
-
-
Manually annotated by BRENDA team
isoform TPS 1
-
-
Manually annotated by BRENDA team
-
Uniprot
Manually annotated by BRENDA team
i.e. Mucormycosis agent or Rhizopus arrhizus var. delemar
UniProt
Manually annotated by BRENDA team
This UniProt-ID has been deleted; strain AS.1416
-
-
Manually annotated by BRENDA team
strain C13-ABYS86 lacking vacuolar proteases
-
-
Manually annotated by BRENDA team
; gene TA1210
UniProt
Manually annotated by BRENDA team
; bifunctional trehalose-6-phosphate synthase/phosphatase, EC 2.4.1.15 and EC 3.1.3.12
SwissProt
Manually annotated by BRENDA team
bifunctional trehalose-6-phosphate synthase/phosphatase, EC 2.4.1.15 and EC 3.1.3.12
SwissProt
Manually annotated by BRENDA team
strain RQ-1, gene otsA
SwissProt
Manually annotated by BRENDA team
strain RQ-1, gene otsA
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP-alpha-D-glucose + D-glucose 6-phosphate
ADP + alpha,alpha-1,1-trehalose 6-phosphate
show the reaction diagram
ADP-alpha-D-glucose + D-glucose 6-phosphate
ADP + alpha,alpha-trehalose 6-phosphate
show the reaction diagram
ADP-glucose + D-glucose 6-phosphate
ADP + alpha,alpha-trehalose 6-phosphate
show the reaction diagram
alpha,alpha-trehalose 6-phosphate + H2O
alpha,alpha-trehalose + phosphate
show the reaction diagram
CDP-glucose + D-glucose 6-phosphate
trehalose 6-phosphate + CDP
show the reaction diagram
dTDP-glucose + glucose 6-phosphate
trehalose 6-phosphate + dTDP
show the reaction diagram
-
-
-
-
?
GDP-alpha-D-glucose + D-glucose 6-phosphate
GDP + alpha,alpha-1,1-trehalose 6-phosphate
show the reaction diagram
GDP-alpha-D-glucose + D-glucose 6-phosphate
GDP + alpha,alpha-trehalose 6-phosphate
show the reaction diagram
GDP-glucose + D-glucose 6-phosphate
trehalose 6-phosphate + GDP
show the reaction diagram
GDP-glucose + glucose 6-phosphate
GDP + alpha,alpha-trehalose 6-phosphate
show the reaction diagram
cf. EC 2.4.1.36
-
-
?
TDP-alpha-D-glucose + D-glucose 6-phosphate
TDP + alpha,alpha-1,1-trehalose 6-phosphate
show the reaction diagram
-
-
-
-
?
TDP-alpha-D-glucose + D-glucose 6-phosphate
TDP + alpha,alpha-trehalose 6-phosphate
show the reaction diagram
TDP-glucose + glucose 6-phosphate
trehalose 6-phosphate + TDP
show the reaction diagram
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + ?
show the reaction diagram
activity with D-fructose 6-phosphate is about 10% compared to the activity with D-glucose 6-phosphate
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + alpha,alpha-1,1-trehalose 6-phosphate
show the reaction diagram
UDP-alpha-D-glucose + D-galactose 6-phosphate
UDP + ?
show the reaction diagram
activity with D-galactose 6-phosphate is about 10% compared to the activity with D-glucose 6-phosphate
-
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-1,1-trehalose 6-phosphate
show the reaction diagram
UDP-alpha-D-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-trehalose 6-phosphate
show the reaction diagram
UDP-alpha-D-glucose + D-mannose 6-phosphate
UDP + ?
show the reaction diagram
activity with D-mannose 6-phosphate is about 40% compared to the activity with D-glucose 6-phosphate
-
-
?
UDP-alpha-D-glucose + D-mannose 6-phosphate
UDP + alpha,alpha-1,1-trehalose 6-phosphate
show the reaction diagram
-
maximum activity with glucose 6-phosphate, followed by mannose 6-phosphate and fructose 6-phosphate
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + ?
show the reaction diagram
-
16% of the activity with D-glucose 6-phosphate
-
-
?
UDP-glucose + D-galactose
UDP + ?
show the reaction diagram
-
17% of the activity with D-glucose 6-phosphate
-
-
?
UDP-glucose + D-glucose
UDP + ?
show the reaction diagram
-
28% of the activity with D-glucose 6-phosphate
-
-
?
UDP-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-trehalose 6-phosphate
show the reaction diagram
UDP-glucose + lactose
UDP + ?
show the reaction diagram
-
10% of the activity with D-glucose 6-phosphate
-
-
?
UDP-glucose + sucrose
UDP + ?
show the reaction diagram
-
19% of the activity with D-glucose 6-phosphate
-
-
?
UDPglucose + glucose 6-phosphate
trehalose 6-phosphate + UDP
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-1,1-trehalose 6-phosphate
show the reaction diagram
-
step in trehalose biosynthesis
-
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-trehalose 6-phosphate
show the reaction diagram
UDP-glucose + D-glucose 6-phosphate
UDP + alpha,alpha-trehalose 6-phosphate
show the reaction diagram
UDPglucose + glucose 6-phosphate
trehalose 6-phosphate + UDP
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
activation
CaCl2
activates 1.44fold at 10 mM; activates 1.44fold at 10 mM
Cd2+
-
activation
Cu2+
-
activates 33% at 5 mM
Fe2+
-
activation
KCl
-
upon an increase in the KCl concentration from 0 to 100 mM, the OtsA activity decreases by more than 40%, whereas it is not significantly affected when UDP or GDP-glucose is used as the substrate
KHCO3
-
100-300 mM, activation
MgCl2
activates 2fold at 10 mM; activates 2fold at 10 mM
MnCl2
-
stimulation
Na+
-
activates at 20 mM
NaHCO3
-
100-300 mM, activation
Ni2+
-
activation
ZnCl2
-
stimulation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
-
1 mM
2,6-diamino-4-(2,4-dichlorophenyl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(2-methylphenyl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(3,4-dichlorophenyl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(3-bromophenyl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(3-chlorophenyl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(3-fluorophenyl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(3-iodophenyl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(4,5-dihydrofuran-3-yl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(4,5-dihydrothiophen-3-yl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(4-bromo-5-ethylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(4-bromo-5-methylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(4-bromothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(4-chlorophenyl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(4-methylphenyl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(4-nitrophenyl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(5-bromothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(5-chlorothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(5-ethylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(5-nitrothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(cyclohex-2-en-1-yl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-(cyclohex-3-en-1-yl)-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-cyclohexyl-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-phenyl-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-[3-(trifluoromethyl)phenyl]-4H-thiopyran-3,5-dicarbonitrile
2,6-diamino-4-[4-(trifluoromethyl)phenyl]-4H-thiopyran-3,5-dicarbonitrile
2-mercaptoethanol
10% (v/v), 9% inhibition; 91% inhibition
3-amino-4-formyl-2-[2-oxo-2-(tricyclo[3.3.1.13,7]dec-1-yl)ethyl]cyclopent-3-ene-1,1,2-tricarbonitrile
6-amino-4-(3,4-dichlorophenyl)-2-thioxo-1,2,3,4-tetrahydropyridine-3,5-dicarbonitrile
8-chloro-11-(piperazin-1-yl)-6,11-dihydro-5H-benzo[5,6]cyclohepta[1,2-b]pyridine
8-hydroxy-2,4-dimethyl-7H-chromen-7-one
Ag+
-
45% inhibition at 5 mM
AMP
-
10 mM 23% inhibition
ATP
-
slight
Ba2+
10 mM, 38% inhibition; 38% inhibition at 10 mM
cathomycin
-
competitive inhibition, inhibits reaction with either UDPglucose or GDPglucose as glucosyl donor. Preincubation with heparin prevents inhibition. 0.05 mg/ml-0.2 mg/ml: 50% inhibition
Cd2+
-
slightly inhibitory
cellobiose
-
-
Cemusol NPT-12
-
weak inhibition
-
circulin
-
noncompetitive inhibition, inhibits reaction with either UDPglucose or GDPglucose as glucosyl donor. Preincubation with heparin prevents inhibition. 0.05 mg/ml-0.2 mg/ml: 50% inhibition
Citric acid
-
2.5 mM, 82% residual activity
D-fructose
-
-
deoxycholate
-
-
dithiothreitol
10 mM, 27% inhibition
Diumycin
-
competitive inhibition, inhibits reaction with either UDPglucose or GDPglucose as glucosyl donor. Preincubation with heparin prevents inhibition. 0.05 mg/ml: 50% inhibition
-
ethanol
10% (v/v), 28% inhibition; 72% inhibition
Fe2+
-
slightly inhibitory
Fe3+
32% inhibition at 10 mM; 32% inhibition at 10 mM
GDPglucose
-
noncompetitive to UDPglucose
glucose 6-phosphate
-
high concentrations
guanidine hydrochloride
10 mM, 63% inhibition; 37% inhibition
Isopropanol
10% (v/v), 14% inhibition; 86% inhibition
methanol
10% (v/v), 30% inhibition; 70% inhibition
methyl 2-chloro-5-(5-[(Z)-[3-(2,4-dimethylphenyl)-4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene]methyl]furan-2-yl)benzoate
Mg2+
-
inhibition in presence of 1 mM UDP or 1 mM UTP
Moenomycin
-
competitive inhibition, inhibits reaction with either UDPglucose or GDPglucose as glucosyl donor. Preincubation with heparin prevents inhibition. Preincubation with heparin prevents inhibition. 0.05 mg/ml: 50% inhibition
n-butanol
10% (v/v), 48% inhibition; 52% inhibition
N-ethylmaleimide
Na+
10 mM, 8% inhibition; 8% inhibition at 10 mM
Ni2+
10 mM, 21% inhibition; 21% inhibition at 10 mM
O,O-diphenyl [(2E)-2-[2-(2-chlorophenyl)hydrazinylidene]-2-cyanoethanethioyl]phosphoramidothioate
phosphate
Poly-D-lysine
-
-
Poly-DL-lysine
-
-
Poly-DL-ornithine
-
-
Poly-L-ornithine
-
-
Polyribonucleotide inhibitor from Mycobacterium tuberculosis
-
-
-
proline
Sodium azide
-
2.5 mM, 83% residual activity
sorbitol
-
-
trehalose
Triton X-100
-
weak inhibition
UDP
-
10 mM, 65% inhibition
UDPglucose
-
competitive to GDPglucose
UDPglucuronate
-
-
UMP
-
10 mM, 43% inhibition
Urea
10 mM, 15% inhibition; 85% inhibition
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Chondroitin
-
stimulation, particularly when a pyrimidine glucose nucleotide is used, rather than a purine glucose nucleotide
chondroitin sulfate
D-fructose 6-phosphate
-
-
dermatan sulfate
-
activation
gamma-Carragenan
-
activation
-
glycosyltransferase
the Thermoproteus tenax trehalose-6-phosphate synthase/phosphatase (TPSP) exhibits high phosphatase activity, but requires activation by the co-expressed glycosyltransferase for bifunctional synthase/phosphatase activity. The glycosyltransferase mediated activation of trehalose-6-phosphate synthase activity relies on the fusion of both, trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase domain, in the TPSP enzyme. Activation is mediated by complex-formation in vivo
-
heparan sulfate
-
activation
heparin
iodoacetamide
iodoacetic acid
Polyanions
-
activation
-
Polynucleotides
-
activation
TPS-activator protein
-
activation
-
trehalose
-
activates up to 25fold
Triton X-100
-
activation
Tween 80
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.6
ADP-glucose
-
37°C, pH 5.5
0.27 - 7.1
D-glucose 6-phosphate
0.4 - 2.17
GDP-glucose
1 - 16
GDPglucose
1 - 8.3
glucose 6-phosphate
3.7 - 7
glucose-6-phosphate
2 - 2.38
TDP-glucose
0.2 - 1
UDP-alpha-D-glucose
0.3 - 9.6
UDP-glucose
0.04 - 18
UDPglucose
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.2 - 6.7
glucose 6-phosphate
3.6 - 7.3
UDPglucose
additional information
additional information
Escherichia coli
-
bifunctional fusion enzyme of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
2
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2 - 5
phosphate
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
2,6-diamino-4-(2,4-dichlorophenyl)-4H-thiopyran-3,5-dicarbonitrile
0.1
2,6-diamino-4-(2-methylphenyl)-4H-thiopyran-3,5-dicarbonitrile
0.0002 - 0.0005
2,6-diamino-4-(3,4-dichlorophenyl)-4H-thiopyran-3,5-dicarbonitrile
0.0012 - 0.0017
2,6-diamino-4-(3-bromophenyl)-4H-thiopyran-3,5-dicarbonitrile
0.0012 - 0.0013
2,6-diamino-4-(3-chlorophenyl)-4H-thiopyran-3,5-dicarbonitrile
0.005 - 0.0061
2,6-diamino-4-(3-fluorophenyl)-4H-thiopyran-3,5-dicarbonitrile
0.0026 - 0.0049
2,6-diamino-4-(3-iodophenyl)-4H-thiopyran-3,5-dicarbonitrile
0.0918 - 0.1
2,6-diamino-4-(4,5-dihydrofuran-3-yl)-4H-thiopyran-3,5-dicarbonitrile
0.0631 - 0.1
2,6-diamino-4-(4,5-dihydrothiophen-3-yl)-4H-thiopyran-3,5-dicarbonitrile
0.1
2,6-diamino-4-(4-bromo-5-ethylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile
0.0008 - 0.1
2,6-diamino-4-(4-bromo-5-methylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile
0.004 - 0.011
2,6-diamino-4-(4-bromothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile
0.0045 - 0.0149
2,6-diamino-4-(4-chlorophenyl)-4H-thiopyran-3,5-dicarbonitrile
0.0129 - 0.0758
2,6-diamino-4-(4-methylphenyl)-4H-thiopyran-3,5-dicarbonitrile
0.0287 - 0.0419
2,6-diamino-4-(4-nitrophenyl)-4H-thiopyran-3,5-dicarbonitrile
0.0045 - 0.023
2,6-diamino-4-(5-bromothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile
0.0059 - 0.0173
2,6-diamino-4-(5-chlorothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile
0.0665 - 0.1
2,6-diamino-4-(5-ethylthiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile
0.0054 - 0.0061
2,6-diamino-4-(5-nitrothiophen-2-yl)-4H-thiopyran-3,5-dicarbonitrile
0.0026 - 0.0029
2,6-diamino-4-(cyclohex-2-en-1-yl)-4H-thiopyran-3,5-dicarbonitrile
0.0031 - 0.0046
2,6-diamino-4-(cyclohex-3-en-1-yl)-4H-thiopyran-3,5-dicarbonitrile
0.0014 - 0.0019
2,6-diamino-4-cyclohexyl-4H-thiopyran-3,5-dicarbonitrile
0.1 - 789
2,6-diamino-4-phenyl-4H-thiopyran-3,5-dicarbonitrile
0.00371 - 0.00505
2,6-diamino-4-[3-(trifluoromethyl)phenyl]-4H-thiopyran-3,5-dicarbonitrile
0.1
2,6-diamino-4-[4-(trifluoromethyl)phenyl]-4H-thiopyran-3,5-dicarbonitrile
0.0014 - 0.0046
3-amino-4-formyl-2-[2-oxo-2-(tricyclo[3.3.1.13,7]dec-1-yl)ethyl]cyclopent-3-ene-1,1,2-tricarbonitrile
0.1
6-amino-4-(3,4-dichlorophenyl)-2-thioxo-1,2,3,4-tetrahydropyridine-3,5-dicarbonitrile
0.0062 - 0.0066
8-chloro-11-(piperazin-1-yl)-6,11-dihydro-5H-benzo[5,6]cyclohepta[1,2-b]pyridine
0.0086 - 0.0314
8-hydroxy-2,4-dimethyl-7H-chromen-7-one
0.0094 - 0.0147
methyl 2-chloro-5-(5-[(Z)-[3-(2,4-dimethylphenyl)-4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene]methyl]furan-2-yl)benzoate
0.0028 - 0.0055
O,O-diphenyl [(2E)-2-[2-(2-chlorophenyl)hydrazinylidene]-2-cyanoethanethioyl]phosphoramidothioate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.01
-
preparation of trehalose-6-phosphate synthase from Saccharomyces cerevisiae, preparation step soluble supernatant
0.011
-
TPS specific activity in glucose, yeast mutant strain tps2delta + pSAL4
0.013
-
TPS specific activity in galactose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1
0.02
-
TPS specific activity in glucose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1
0.055
-
TPS specific activity in galactose, yeast wild type + pSAL4
0.063
-
TPS specific activity in galactose, yeast mutant strain tps2delta + pSAL4::TPS2
0.067
-
TPS specific activity in glucose, yeast mutant strain tps2delta + pSAL4::TPS2
0.073
-
TPS specific activity in glucose, yeast mutant strain tps2delta + pSAL4::TPS1-TPS2
0.078
-
TPS specific activity in galactose, yeast mutant strain tps2delta + pSAL4::TPS1-TPS2
0.079
-
TPS specific activity in glucose, yeast wild type + pSAL4
0.081
-
TPS specific activity in galactose, yeast mutant strain tps1delta + pSAL4::TPS1-TPS2
0.086
-
TPS specific activity in glucose, yeast mutant strain tps1delta + pSAL4::TPS1-TPS2
0.088
-
TPS specific activity in galactose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1-TPS2
0.093
-
TPS specific activity in glucose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1-TPS2
0.096
-
crude enzyme, pH 8.5, 37°C
0.127
-
UDPglucose
0.144
-
TPS specific activity in glucose, yeast mutant strain tps1delta + pSAL4::TPS1
0.163
-
TPS specific activity in galactose, yeast mutant strain tps1delta + pSAL4::TPS1
0.49
-
preparation of trehalose-6-phosphate synthase from Saccharomyces cerevisiae, preparation step ammonimum sulfate fractionation
0.74
-
preparation of trehalose-6-phosphate synthase from Saccharomyces cerevisiae, preparation step HPGPLC on TSK G2000SW
1.48
-
pH 8.5, 37°C
2.03
-
purified native enzyme, pH 8.5, 37°C
3.5
pH 7.0, 80°C
4.8
-
activity with UDPglucose
7.05
-
purified enzyme
7.33
purified recombinant enzyme, pH 6.0, 60°C, substrate UDP-glucose
20.6
purified recombinant enzyme, substrates GDP-glucose and D-glucose 6-phosphate
24.2
-
activity with GDPglucose
95
purified recombinant enzyme, substrates ADP-glucose and D-glucose 6-phosphate
106.7
purified recombinant enzyme, substrates TDP-glucose and D-glucose 6-phosphate
149.6
purified recombinant enzyme, substrates UDP-glucose and D-glucose 6-phosphate
150.5
purified native enzyme, pH 4.0, 37°C; purified native enzyme, pH 4.0, 37°C
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 7
activity range; activity range
4 - 9
activity range, profile overview
4.5 - 10.5
-
TPS activity is maximally at pH 8.5 with 2.11 U/mg, followed by 2.01 U/mg at pH 9.0, 1.93 U/mg at pH 9.5, and 1.21 U/mg at pH 10.5, but only 0.31 U/mg at pH 4.5
5 - 6
-
pH 5.0: about 85% of maximal activity, pH 6.0: about 50% of maximal activity
5 - 7
pH 5.0: about 50% of maximal activity, pH 7.0: about 50% of maximal activity
5.5 - 8
-
pH 5.5: about 75% of maximal activity, pH 8.0: about 70% of maximal activity
6 - 8
drastic decrease in enzyme activity is observed at pH values below pH 6.0 and above pH 8.0
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 20
catalytic activity of OtsA at 4°C is 1.2fold greater than that at 20°C
16 - 37
tested activity range
20 - 50
-
20% of maximal activity at 20°C and 50°C, inactive at 65°C
20 - 60
activity range, at 40°C, the enzyme demonstrates 87.04% of its maximum activity. A rapid decrease in the enzyme activity occurs at 50°C with 35.27% of maximum activity, completely loss of activity above 60°C; activity range, at 40°C, the enzyme demonstrates 87.04% of its maximum activity. A rapid decrease in the enzyme activity occurs at 50°C with 35.27% of maximum activity, completely loss of activity above 60°C
20 - 50
drastic decrease in enzyme activity is observed at temperatures below 20°C and above 50°C
40 - 100
about half-maximal activity at 40°C, 80% of maximal activity at 100°C, inactive at 30°C
50 - 80
50°C: about 50% of maximal activity, 80°C: about 75% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.55
-
115 kDa enzyme polypeptide, isoelectric focusing
4.69
-
67 kDa enzyme polypeptide, isoelectric focusing
4.83
-
50 kDa enzyme polypeptide, isoelectric focusing
5.4
isoelectric focusing; isoelectric focusing
5.83
calculated from sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
in both males and females, the darker muscles exhibit greater amounts of trehalose, trehalose 6-phosphate synthase, and trehalase activities than the light colored; in both males and females, the darker muscles exhibit greater amounts of trehalose, trehalose 6-phosphate synthase, and trehalase activities than the light colored; in both males and females, the darker muscles exhibit greater amounts of trehalose, trehalose 6-phosphate synthase, and trehalase activities than the light colored; in both males and females, the darker muscles exhibit greater amounts of trehalose, trehalose 6-phosphate synthase, and trehalase activities than the light colored
Manually annotated by BRENDA team
correlation between TPS activity in hemocytes and hemolymph trehalose levels is found during the molt cycle
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
the enzyme is markedly present in the vacuole and the cell wall, and to a lesser extent in the cytosol of transgenic Nicotiana tabacum
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Burkholderia cepacia (strain R1808)
Burkholderia multivorans (strain ATCC 17616 / 249)
Burkholderia vietnamiensis (strain G4 / LMG 22486)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487)
Escherichia coli (strain K12)