Information on EC 2.4.1.146 - beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.146
-
RECOMMENDED NAME
GeneOntology No.
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->3)-[N-acetyl-D-glucosaminyl-(1->6)]-N-acetyl-D-galactosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-D-galactosaminyl-R
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mucin core 1 and core 2 O-glycosylation
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-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->3)-[N-acetyl-D-glucosaminyl-(1->6)]-N-acetyl-D-galactosaminyl-R 3-beta-N-acetyl-D-glucosaminyltransferase
cf. EC 2.4.1.102 (beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase), EC 2.4.1.147 (acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase) and EC 2.4.1.148 (acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
87927-99-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
no activity in Canis familiaris
submaxillary gland
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
O-glycan biosynthesis pathways and proposed O-glycan structures of selected glycosyltransferases, overview. Core 1 dominates the O-glycan repertoire. Core 1 can be further elongated by extended C1 beta3GnT3 enzyme to form extended core 1 structure, or be modified by C2 beta6GnT1 enzyme, EC 2.4.1.102, to form core 2 O-glycans. Regulation of O-glycan biosynthesis, overview. The extended C1 beta3GnT3 is competing withthe endogenous ST3Gal
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + antifreeze glycoprotein
UDP + N-acetyl-beta-D-glucosaminyl-1,3-antifreeze glycoprotein
show the reaction diagram
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-benzyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-benzyl
show the reaction diagram
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-o-nitrophenyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-o-nitrophenyl
show the reaction diagram
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-p-nitrophenyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-p-nitrophenyl
show the reaction diagram
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-p-nitrophenyl
UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-p-nitrophenyl
show the reaction diagram
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R
UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + methyl 9-[[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-2)-6-deoxy-alpha-D-mannopyranosyl-(1-3)-[6-deoxy-alpha-D-mannopyranosyl-(1-6)]-beta-D-mannopyranosyl]oxy]nonanoate
UDP + methyl 9-[[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-2)-6-deoxy-alpha-D-mannopyranosyl-(1-3)-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-2)-6-deoxy-alpha-D-mannopyranosyl-(1-6)]-beta-D-mannopyranosyl]oxy]nonanoate
show the reaction diagram
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synthetic hydrophobic tetrasaccharide substrate
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
activation
additional information
-
no activation by Ca2+, Mg2+, Zn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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83% inhibition at 10 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
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stimulation, 0.1% v/v
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2
beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-benzyl
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0.9
beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-o-nitrophenyl
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-
0.13
methyl 9-[[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1-2)-6-deoxy-alpha-D-mannopyranosyl-(1-3)-[6-deoxy-alpha-D-mannopyranosyl-(1-6)]-beta-D-mannopyranosyl]oxy]nonanoate
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1.6
UDP-N-acetyl-D-glucosamine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000007
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CaCo-2, colonic adenocarcinoma, cells differentiated to enterocytes
0.0000015
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undifferentiated CaCo-2, colonic adenocarcinoma, cells
0.0000033
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below, EBV immortilized B-cell line, from normal patients and such with the Wiskott-Aldrich syndrome
0.000006
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normal granulocytes
0.000032
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additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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EBV immortilized B-cell line, from normal patients and such with the Wiskott-Aldrich syndrome
Manually annotated by BRENDA team
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during progression to cancer
Manually annotated by BRENDA team
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low level, in normal, but not in leukemic cells
Manually annotated by BRENDA team
high endothelial venule of secondary lymphoid organs
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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and monomer, PAGE and SDS-PAGE
monomer
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and dimer, PAGE and SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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secreted protein is more heavily glycosylated and sialylated than its membrane-associated counterparts
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, microsomal pig stomach enzyme preparation, detergent-free 0.25 M sucrose suspension, several years
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning of a sequence fragment encoding the soluble catalytic domain, expression as His-tagged protein in CHO cells; isolation and cloning of full length DNA via EST and expression in CHO cells
gene B3GNT3, recombinant expression in CHO-K1 cells. Glycosylation-related genes in CHO-K1 cells are present, but strictly suppressed and regulated. CHO cells are transiently co-transfected with plasmids encoding extended C1 beta3GnT3, C2 beta6GnT1, or C3 beta3GnT6 with or without ST6Gal I or CHST4, respectively, and resulting O-glycan and core chain spectrum, overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
use of the recombinant enzyme for construction of the 6-sulfo sialyl Lewis x on extended core1 O-glycans