Information on EC 2.4.1.138 - mannotetraose 2-alpha-N-acetylglucosaminyltransferase

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The expected taxonomic range for this enzyme is: Kluyveromyces lactis

EC NUMBER
COMMENTARY hide
2.4.1.138
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RECOMMENDED NAME
GeneOntology No.
mannotetraose 2-alpha-N-acetylglucosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-D-glucosamine + (1->3)-alpha-D-mannosyl-(1->2)-alpha-D-mannosyl-(1->2)-alpha-D-mannosyl-D-mannose = UDP + (1->3)-alpha-D-mannosyl-(1->2)-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-(1->2)-alpha-D-mannosyl-D-mannose
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->2)-alpha-D-mannosyl-(1->2)-D-mannose alpha-N-acetyl-D-glucosaminyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
81032-47-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain KL8
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
requirement, 10 mM, inhibits at higher concentrations, Mg2+ or Ca2+ cannot replace Mn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
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strong, 1 mM, even in the presence of Mn2+, wild-type
Cu2+
-
wild-type
imidazole
-
wild-type
Mn2+
-
above 10 mM, wild-type
phosphate
-
wild-type, complete inhibition at 40 mM
Tris
-
wild-type
UDP-hexylamine
-
wild-type
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11 - 13
mannotetraose acceptor
-
0.0364 - 0.0447
UDP-N-acetylglucosamine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
activity of wild-type and mutant strains with different acceptors
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
pI: 4.9, in the presence of Triton X-100 and 2 M urea
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
integral membrane protein
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
300000
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gel filtration in the presence of Triton X-100, native MW may be lower
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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possesses no N-acetylglucosamine residues, enzyme does not bind to wheat germ agglutinin
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
2 h, inactivation
45
-
t1/2: 4 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Triton X-100 stabilizes solubilized enzyme
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
organic solvent extraction, unstable to
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, in imidazole-glycerol buffer, 2% Triton X-100, t1/2: 80 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information