Information on EC 2.4.1.127 - monoterpenol beta-glucosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Mentha x piperita

EC NUMBER
COMMENTARY hide
2.4.1.127
-
RECOMMENDED NAME
GeneOntology No.
monoterpenol beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + (-)-menthol = UDP + (-)-menthyl O-beta-D-glucoside
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:(-)-menthol O-beta-D-glucosyltransferase
(+)-Neomenthol can also act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
78990-64-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cv. Black Mitcham
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDPglucose + D-neomenthol
UDP + D-neomenthyl-beta-D-glucoside
show the reaction diagram
-
-
-
?
UDPglucose + L-menthol
UDP + L-menthyl-beta-D-glucoside
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPglucose + L-menthol
UDP + L-menthyl-beta-D-glucoside
show the reaction diagram
-
enzyme is involved in metabolism of L-menthone
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
20 mM, about 30% stimulation
MnCl2
-
slight stimulation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
5 mM, strong
CdCl2
-
strong
Co2+
-
5 mM, strong
HgCl2
-
strong
Ni2+
-
5 mM, strong
p-hydroxymercuribenzoate
-
strong
Zn2+
-
5 mM, strong
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
without addition 73% decrease of activity, suggesting a thiol function necessary for activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.048
D-neomenthol
-
-
0.059
L-menthol
-
-
0.2 - 0.25
UDPglucose
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3
-
70 mM Tris-sodium maleate buffer
7.7
-
70 mM Na-phosphate buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8.5
-
about 50% of maximal activity at pH 6.0 and pH 8.5, 70 mM Tris-sodium maleate buffer
7.3 - 8.3
-
about 50% of maximal activity at pH 7.3 and pH 8.3, 70 mM Na-phosphate buffer
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46000
-
gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE