Information on EC 2.4.1.121 - indole-3-acetate beta-glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.121
-
RECOMMENDED NAME
GeneOntology No.
indole-3-acetate beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + (indol-3-yl)acetate = UDP + 1-O-(indol-3-yl)acetyl-beta-D-glucose
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
indole-3-acetate inactivation IX
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superpathway of indole-3-acetate conjugate biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:(indol-3-yl)acetate beta-D-glucosyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
74082-56-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
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the enzyme belongs to the UDP-sugar dependent transferases
metabolism
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the enzyme catalyzes the first step in the biosynthesis of indol-3-yl ester conjugates in monocotyledonous plants
physiological function
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1-O-(indol-3-yl)acetyl-beta-D-glucose, synthesized by the enzyme, is the substrate for (indol-3-yl)acetate labeling of glycoproteins from pea seeds indicating a possible role of this enzyme in the covalent modification of a class of proteins by a plant hormone. Auxin is a key plant hormone that regulates many physiological processes in plants including organ morphogenesis (i.e. apical dominance, root development) and environmental responses (biotic and abiotic stress). The major auxin is indole-3-acetic acid (IAA), but other indole-derived carboxylic acids, i.e. indole-3-butyric acid, indole-3-propionic acid, 4-chloro-indole-3-acetic acid, and indole-3-pyruvic acid have also auxin activity. Auxin action is highly dependent on its concentration, which must be precisely controlled
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucose + (indol-3-yl)acetate
UDP + 1-O-(indol-3-yl)acetyl-beta-D-glucose
show the reaction diagram
UDP-glucose + (indol-3-yl)propionate
UDP + 1-O-(indol-3-yl)propionyl-beta-D-glucose
show the reaction diagram
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22% of the activity with (indol-3-yl)acetate
-
-
r
UDP-glucose + 1-naphthalene acetate
UDP + 1-naphthalene acetyl-beta-D-glucose
show the reaction diagram
-
24% of the activity with (indol-3-yl)acetate
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-
r
UDP-glucose + gibberellin GA3
UDP + ?
show the reaction diagram
-
1.9% of the activity with (indol-3-yl)acetate
-
-
r
UDP-glucose + indole-3-acetate
1-O-indole-3-acetyl-beta-D-glucose + UDP
show the reaction diagram
UDPglucose + 2,5-dimethoxycinnamic acid
UDP + 2,5-dimethoxycinnamoyl-beta-1-D-glucose
show the reaction diagram
-
4.3% of the activity with indole-3-acetate
-
-
?
UDPglucose + 3,4-dimethoxycinnamic acid
UDP + 3,4-dimethoxycinnamoyl-beta-1-D-glucose
show the reaction diagram
-
6.1% of the activity with indole-3-acetate
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-
?
UDPglucose + 3-hydroxy-4-methoxy cinnamic acid
UDP + 3-hydroxy-4-methoxycinnamoyl-beta-1-D-glucose
show the reaction diagram
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9.4% of the activity with indole-3-acetate
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-
?
UDPglucose + ferulic acid
UDP + feruloyl-beta-1-D-glucose
show the reaction diagram
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7.8% of the activity with indole-3-acetate
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-
?
UDPglucose + indole butyric acid
UDP + indole butanoyl-beta-1-D-glucose
show the reaction diagram
-
-
-
-
?
UDPglucose + indole propionic acid
UDP + indole propanoyl-beta-1-D-glucose
show the reaction diagram
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-
-
-
?
UDPglucose + indole-3-acetate
?
show the reaction diagram
UDPglucose + indole-3-acetate
UDP + indole-3-acetyl-beta-1-D-glucose
show the reaction diagram
UDPglucose + m-hydroxycinnamic acid
UDP + m-hydroxycinnamoyl-beta-1-D-glucose
show the reaction diagram
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4.3% of the activity with indole-3-acetate
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-
?
UDPglucose + naphthalene-1-acetic acid
UDP + naphthyl-1-acetyl-beta-1-D-glucose
show the reaction diagram
UDPglucose + o-hydroxycinnamic acid
UDP + o-hydroxycinnamoyl-beta-1-D-glucose
show the reaction diagram
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7.6% of the activity with indole-3-acetate
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-
?
UDPglucose + p-coumaric acid
UDP + p-coumaroyl-beta-1-D-glucose
show the reaction diagram
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10.2% of the activity with indole-3-acetate
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-
?
UDPglucose + phenylacetic acid
UDP + phenylacetyl-beta-1-D-glucose
show the reaction diagram
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-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + (indol-3-yl)acetate
UDP + 1-O-(indol-3-yl)acetyl-beta-D-glucose
show the reaction diagram
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-
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r
UDPglucose + indole-3-acetate
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
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stimulates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4-dichlorophenoxy acetic acid
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2-naphthalene acetic acid
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competitive inhibitor
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abscisic acid
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weak
diphosphate
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gibberellic acid
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weak
indole-3-acetate glucan
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indole-3-acetate-myo-inositol
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indole-3-butyric acid
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competitive inhibitor
Indole-3-propionic acid
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competitive inhibitor
kinetin
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weak
naphthylphthalamic acid
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phosphate
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100 mM phosphate buffer, 50% inhibition
phosphatidyl ethanolamine
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zeatin
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Calmodulin
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stimulates
D-gluconic acid lactone
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the inhibitor of beta-glucosidase activity, potentiates the enzyme activity at the optimal concentration of 0.3 mM
thiol compounds
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stimulate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.52
(indol-3-yl)acetate
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pH 7.6, 30C
1.5
(indol-3-yl)propionate
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pH 7.6, 30C
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4
1-naphthalene acetate
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pH 7.6, 30C
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0.18
Cinnamic acid
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0.15
indole butyric acid
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0.14
indole propionic acid
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0.24 - 1.08
indole-3-acetate
2.67
UDP-glucose
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pH 7.6, 30C
0.25 - 2.5
UDPglucose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.27
(indol-3-yl)acetate
Pisum sativum
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pH 7.6, 30C
0.72
(indol-3-yl)propionate
Pisum sativum
-
pH 7.6, 30C
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0.53
1-naphthalene acetate
Pisum sativum
-
pH 7.6, 30C
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0.49
UDP-glucose
Pisum sativum
-
pH 7.6, 30C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.36
(indol-3-yl)acetate
Pisum sativum
-
pH 7.6, 30C
6264
0.46
(indol-3-yl)propionate
Pisum sativum
-
pH 7.6, 30C
210483
0.13
1-naphthalene acetate
Pisum sativum
-
pH 7.6, 30C
210484
0.16
UDP-glucose
Pisum sativum
-
pH 7.6, 30C
64
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.038
2-naphthalene acetic acid
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pH 7.6, 30C
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0.1
indole-3-butyric acid
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pH 7.6, 30C
0.15
Indole-3-propionic acid
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pH 7.6, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.06
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purified enzyme, substrate gibberellin GA3, pH 7.6, 30C
0.656
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purified enzyme, substrate (indol-3-yl)propionate, pH 7.6, 30C
0.715
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purified enzyme, substrate 1-naphthalene acetate, pH 7.6, 30C
0.798
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2.976
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purified enzyme, substrate (indol-3-yl)acetate, pH 7.6, 30C
4.029
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additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1
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7.3 - 7.6
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7 - 7.6
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pH 7.3-7.6: maximal activity, no activity below pH 6.7
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43700
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x * 43700, SDS-PAGE
45700
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x * 45700, SDS-PAGE
46500
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gel filtration
51000
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1 * 51000, SDS-PAGE
52000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 51000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
loses activity during column chromatographic procedures
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retention of 79% of the initial activity after 16 h dialysis
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stable to lyophilization
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, stable for 15 d, 15% loss of activity after 35 d
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme from immature seeds by PEG fractionation, anion exchange chromatography, and preparative PAGE
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partial
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recombinant enzyme
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