Information on EC 2.4.1.117 - dolichyl-phosphate beta-glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.117
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RECOMMENDED NAME
GeneOntology No.
dolichyl-phosphate beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + dolichyl phosphate = UDP + dolichyl beta-D-glucosyl phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
N-Glycan biosynthesis
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protein N-glycosylation (eukaryotic, high mannose)
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dolichyl-diphosphooligosaccharide biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:dolichyl-phosphate beta-D-glucosyltransferase
Solanesyl phosphate and ficaprenyl phosphate can act as acceptors, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
71061-42-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
calf
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
mung bean
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Manually annotated by BRENDA team
L. inbred A 636
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucose + dolichyl phosphate
UDP + dolichyl beta-D-glucosyl phosphate
show the reaction diagram
UDP-glucose + ficaprenyl phosphate
UDP + ficaprenyl beta-D-glucosyl phosphate
show the reaction diagram
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-
-
-
r
UDP-glucose + solanesyl phosphate
UDP + solanesyl beta-D-glucosyl phosphate
show the reaction diagram
-
-
-
-
r
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + dolichyl phosphate
UDP + dolichyl beta-D-glucosyl phosphate
show the reaction diagram
-
-
-
-
r
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
divalent cation required, efficiency of activiation in descending order: Mg2+, Mn2+, Ca2+, Co2+, Zn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Amphomycin
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IC50 0.04 mM
Ca2+
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slight inhibition
Mn2+
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slight inhibition
MnCl2
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stimulates at 1 mM, inhibits at higher concentration
tunicamycin
UDP-glucuronic acid
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-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Detergent
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required for maximum activity, optimum for Triton X-100: 0.015%
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Phospholipid
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required, phosphatidylethanolamine most effective
Reducing agent
-
required
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Triton X-100
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required for optimal activity, 0.01-0.015%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0012 - 0.0045
dolichyl phosphate
0.00015 - 0.104
UDP-glucose
additional information
additional information
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1 - 0.17
UDP
0.0076
UDP-glucuronic acid
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-
0.1 - 0.66
UMP
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04
Amphomycin
Candida albicans
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IC50 0.04 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3
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second optimum at pH 7.2
6.5
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7.2
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second optimum at pH 5.3
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6 - 8.8
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pH 5.6: 25% of maximum activity, pH 8.8: about 40% of maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36000
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x * 36000, SDS-PAGE
38000
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x * 38000, deduced from gene sequence
39000
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x * 39000, SDS-PAGE after photoaffinity labeling, catalytic subunit
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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2 h, 50% loss of activity
52
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10 min, complete loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
uridine or UDPglucuronic acid, 5 mM, protects solubilized enzyme against rapid inactivation
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, indefinitely stable below
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-70°C, up to 4 weeks
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4°C, stable for several days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
5 mM uridine or UDPglucuronic acid reconstitutes inactivated enzyme
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