We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Isolated from the plants Silene dioica (red campion) , Iris ensata (Japanese iris) and Iris hollandica (Dutch iris) . Also acts on the 3-O-rutinosides of pelargonidin, delphinidin and malvidin, but not the corresponding glucosides or 6-acylglucosides. The enzyme does not catalyse the glucosylation of the 5-hydroxy group of cyanidin 3-glucoside.
The enzyme appears in viruses and cellular organisms
Synonyms
cyanidin-3-rhamnosylglucoside 5-O-glucosyltransferase, EC 2.4.1.235, glucosyltransferase, uridine diphosphoglucose-cyanidin 3-rhamnosylglucoside 5-O-, UDP-glucose:anthocyanidin 3-rhamnosylglucoside 5-O-glucosyltransferase, UDP-glucose:cyanidin-3-rhamnosyl-(1-6)-glucoside-5-O-glucosyltransferase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cyanidin-3-rhamnosylglucoside 5-O-glucosyltransferase
-
-
-
-
EC 2.4.1.235
-
-
formerly
-
glucosyltransferase, uridine diphosphoglucose-cyanidin 3-rhamnosylglucoside 5-O-
-
-
-
-
UDP-glucose:anthocyanidin 3-rhamnosylglucoside 5-O-glucosyltransferase
-
-
UDP-glucose:cyanidin-3-rhamnosyl-(1-6)-glucoside-5-O-glucosyltransferase
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose + cyanidin-3-O-rutinoside = UDP + cyanidin 3-O-rutinoside 5-O-beta-D-glucoside
UDP-alpha-D-glucose + cyanidin-3-O-rutinoside = UDP + cyanidin 3-O-rutinoside 5-O-beta-D-glucoside
-
-
-
-
UDP-alpha-D-glucose + cyanidin-3-O-rutinoside = UDP + cyanidin 3-O-rutinoside 5-O-beta-D-glucoside
also acts on pelargonidin-3-rutinoside, the enzyme does not catalyse the glucosylation of the 5-hydroxy group of cyanidin-3-glucoside
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hexosyl group transfer
-
-
-
-
transfer of glycosyl group
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose:cyanidin-3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside 5-O-beta-D-glucosyltransferase
Isolated from the plants Silene dioica (red campion) [1], Iris ensata (Japanese iris) [2] and Iris hollandica (Dutch iris) [3]. Also acts on the 3-O-rutinosides of pelargonidin, delphinidin and malvidin, but not the corresponding glucosides or 6-acylglucosides. The enzyme does not catalyse the glucosylation of the 5-hydroxy group of cyanidin 3-glucoside.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
anthocyanidin + UDP-D-glucose
anthocyanidin 5-O-glucoside + UDP
-
-
-
-
?
anthocyanidin 5-O-glucoside + UDP-D-glucose
anthocyanidin-3,5-O-diglucoside + UDP
-
-
-
-
?
UDP-alpha-D-glucose + anthocyanidin 3-O-beta-D-rutinoside
UDP + anthocyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside
-
at pH 7.4, substrate specificity is pH-dependent
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-rutinoside
UDP + cyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside
UDP-alpha-D-glucose + pelargonidin 3-O-(6-O-alpha-L-rhamnosyl)-beta-D-glucoside
UDP + pelargonidin 3-O-(6-O-alpha-L-rhamnosyl)-beta-D-glucosyl-5-O-beta-D-glucoside
-
-
-
?
UDP-glucose + anthocyanidin-3-O-glucoside
UDP + anthocyanidin-3,5-O-diglucoside
-
at pH 6.5, but not at pH 7.4, substrate specificity is pH-dependent
-
-
?
UDP-glucose + cyanidin-3-O-D-rhamnosyl-1,6-D-glucoside
UDP + cyanidin-3-O-[D-rhamnosyl-1,6-D-glucoside]-5-O-D-glucoside
-
-
-
-
r
UDP-glucose + cyanidin-3-O-glucoside
UDP + cyanidin-3,5-diglucoside
UDP-glucose + pelargonidin-3-rutinoside
UDP + pelargonidin-3-rutinoside-5-O-beta-D-glucoside
-
also uses pelargonidin-3-rhamnosylglucoside, glucosylates the 5-hydroxyl group
-
-
?
UDPglucose + cyanidin 3-O-D-glucoside
UDP + ?
-
-
depending upon pH, the enzyme catalyzes the formation of either anthocyanidin 3-rhamnosylglucoside or 3,5-diglucoside. Maximal formation of anthocyanidin 3-rhamnosylglucoside-5-gluside at pH 7.4, maximal formation of anthocyanidin 3,5-diglucoside at pH 6.5. Formation of cyanidin 3-rhamnosylglucoside-5-glucoside proceeds 5-10fold faster than the cyanidin 3,5-diglucoside formation
?
UDPglucose + cyanidin 3-O-D-rhamnosylglucoside
UDP + ?
-
-
depending upon pH, the enzyme catalyzes the formation of either anthocyanidin 3-rhamnosylglucoside or 3,5-diglucoside. Maximal formation of anthocyanidin 3-rhamnosylglucoside-5-gluside at pH 7.4, maximal formation of anthocyanidin 3,5-diglucoside at pH 6.5
?
UDPglucose + cyanidin-3-O-D-rhamnosyl-1,6-D-glucoside
UDP + cyanidin-3-O-[D-rhamnosyl-1,6-D-glucoside]-5-O-D-glucoside
additional information
?
-
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-rutinoside
UDP + cyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside
-
biosynthetic pathway of cyanidin-3-rhamnosylglucoside-5-glucoside, enzyme activity is controlled by a single dominant gene M
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-rutinoside
UDP + cyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside
-
catalyzes the transfer of the glucosyl moiety of UDP-glucose to the 5-hydroxyl group of cyanidin-3-rhamnosyl-(1?6)-glucoside
-
-
?
UDP-glucose + cyanidin-3-O-glucoside
UDP + cyanidin-3,5-diglucoside
-
-
-
-
?
UDP-glucose + cyanidin-3-O-glucoside
UDP + cyanidin-3,5-diglucoside
-
at a much lower rate than cyanidin-3-O-rutinoside, at pH 6.5, but not at pH 7.4, enzyme can use different substrates for 5-O-glycosylation depending on the pH
-
-
?
UDPglucose + cyanidin-3-O-D-rhamnosyl-1,6-D-glucoside
UDP + cyanidin-3-O-[D-rhamnosyl-1,6-D-glucoside]-5-O-D-glucoside
-
no activity with ADPglucose
-
?
UDPglucose + cyanidin-3-O-D-rhamnosyl-1,6-D-glucoside
UDP + cyanidin-3-O-[D-rhamnosyl-1,6-D-glucoside]-5-O-D-glucoside
-
involved in anthocyan biosynthesis. Activity is controlled by a single dominant gene
-
-
?
additional information
?
-
-
no substrate: anthocyanidin 3-O-glucoside
-
-
?
additional information
?
-
-
not: ADP-glucose, enzyme can use different substrates for 5-O-glycosylation depending on the pH
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-rutinoside
UDP + cyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside
-
biosynthetic pathway of cyanidin-3-rhamnosylglucoside-5-glucoside, enzyme activity is controlled by a single dominant gene M
-
-
?
UDPglucose + cyanidin-3-O-D-rhamnosyl-1,6-D-glucoside
UDP + cyanidin-3-O-[D-rhamnosyl-1,6-D-glucoside]-5-O-D-glucoside
-
involved in anthocyan biosynthesis. Activity is controlled by a single dominant gene
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
enzyme is stimulated by divalent metal ions
CaCl2
-
1 mM, 1.6fold stimulation of glucosyl transfer to anthocyanidin-3-O-rutinoside at pH 7.4, but not of glucosyl transfer to anthocyanidin-3-O-glucoside at pH 6.5
CaCl2
-
1 mM, 180% stimulation, highest stimulation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
HgCl2
-
0.1 mM, 95% inhibition, irreversible inhibition
N-ethylmaleimide
-
3 mM, 25% inhibition
NEM
-
3 mM, 25% inhibition, 2-mercpatoethanol or cysteine does not protect
p-chloromercuribenzoate
-
3 mM, 25% inhibition
PCMB
-
3 mM, 25% inhibition, 2-mercpatoethanol or cysteine does not protect
EDTA
-
1 mM, 57% inhibition
EDTA
-
1 mM, pH 7.5, inhibits formation of cyanidin 3-rhamnosylglucoside-5-glucoside but has no effect on the formation of cyanidin 3,5-diglucoside at pH 6.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Polyvinylpyrrolidone
-
soluble, required for maximal activity in crude extracts
Ca2+
-
1 mM, highest stimulation of divalent cations
Ca2+
-
1 mM CaCl2 stimulates 5-O-glucosylation of cyanidin 3-rhamnosyl-glucoside 1.6fold, pH 7.4
additional information
-
glucosylation of cyanidin 3-glucoside at pH 6.5 is not stimulated by divalent metal ions
-
additional information
-
influence of detergents and reducing agents on enzyme activity in crude extracts
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2 - 3.4
anthocyanidin-3-O-glucoside
-
pH 6.5
3.6
anthocyanidin-3-O-rutinoside
-
pH 7.4
23.4
cyanidin-3-glucoside
-
-
3.6
cyanidin-3-O-D-rhamnosyl-1,6-D-glucoside
-
-
3.6
cyanidin-3-O-rutinoside
-
pH 7.4, 30°C
3.6
cyanidin-3-rhamnosylglucoside
-
-
0.5
UDP-glucose
-
pH 7.4, 30°C
0.6
UDP-glucose
-
pH 6.5 and pH 7.4
0.5
UDPglucose
-
-
0.5
UDPglucose
-
reaction with cyanidin 3-rhamnosylglucoside at pH 7.4
0.59
UDPglucose
-
reaction with + cyanidin 3-rhamnosylglucoside at pH 6.5
0.67
UDPglucose
-
reaction with cyanidin-3-glucoside at pH 6.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00953
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6.5
-
activity with cyanidin-3-glucoside
6.5
-
glucosyl transfer to anthocyanidin-3-O-glucoside, substrate specificity is pH-dependent
6.5
-
glucosyl transfer to cyanidin-3-O-glucoside, substrate specificity is pH-dependent
7.4
-
-
7.4
-
activity with cyanidin-3-rhamnosylglucoside
7.4
-
glucosyl transfer to anthocyanidin 3-O-rutinoside, substrate specificity is pH-dependent
7.4
-
glucosyl transfer to cyanidin-3-O-rutinoside and pelargonidin-3-rutinoside, substrate specificity is pH-dependent
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6 - 7.3
-
pH 6.0: about 80% of maximal activity, pH 7.3: about 50% of half-maximal activity, reaction with cyanidin 3-glucoside
7 - 8.5
-
about half-maximal activity at pH 7 and 8.5, reaction with cyanidin-3-rhamnosylglucoside
6.9 - 8.8
-
about 50% of maximal activity at pH 6.9 and at pH 8.8
6.9 - 8.8
-
half-maximum velocities at pH 6.9 and 8.8, cyanidin-3-O-rutinoside as substrate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
enzyme has activity of both EC 2.4.1.115 and EC 2.4.1.116
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
not in green parts of the plant
brenda
-
-
brenda
-
not in green parts of the plant
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
IH5GT_IRIHO
463
0
50129
Swiss-Prot
Mitochondrion (Reliability: 4 )
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7.6
-
30 min, stable below
488479
8
-
30 min, about 10% loss of activity
488479
8.5
-
30 min, about 40% loss of activity
488479
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
repeated freeze-thawing inactivates
-
repeated freezing and thawing leads to a rapid decline in activity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-20°C, about 60-80% loss of activity within 3 months
-
-20°C, concentrated enzyme preparation, 3 months, 60-80% loss of activity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Kamsteeg, J.; van Brederode, J.; van Nigtevecht, G.
Identification, properties, and genetic control of UDP-glucose: cyanidin-3-rhamnosyl-(1 -6)-glucoside-5-O-glucosyltransferase isolated from petals of the red campion (Silene dioica)
Biochem. Genet.
16
1059-1071
1978
Silene dioica
brenda
Kamsteeg, J.; van Brederode, J.; van Nigtevecht, G.
The pH-dependent substrate specificity of UDP-glucose: anthocyandin 3-rhamnosylglucoside, 5-O-glucosyltransferase in petals of Silene dioica: the formation of anthocyanidin 3,5-diglucosides
Z. Pflanzenphysiol.
96
87-93
1980
Silene dioica
-
brenda
Ogata, J.; Kanno, Y.; Itoh, Y.; Tsugawa, H.; Suzuki, M.
Plant biochemistry: Anthocyanin biosynthesis in roses
Nature
435
757-758
2005
Rosa hybrid cultivar
brenda
Select items on the left to see more content.
html completed