Information on EC 2.4.1.114 - 2-coumarate O-beta-glucosyltransferase

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The expected taxonomic range for this enzyme is: Melilotus albus

EC NUMBER
COMMENTARY hide
2.4.1.114
-
RECOMMENDED NAME
GeneOntology No.
2-coumarate O-beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + trans-2-hydroxycinnamate = UDP + trans-beta-D-glucosyl-2-hydroxycinnamate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
coumarin biosynthesis (via 2-coumarate)
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-
Phenylpropanoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:trans-2-hydroxycinnamate O-beta-D-glucosyltransferase
Coumarinate (cis-2-hydroxycinnamate) does not act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
73665-97-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 2-hydroxycinnamate
UDP + (2E)-3-[2-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 2-hydroxycinnamate
UDP + (2E)-3-[2-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
-
1 mM, 63% inhibition
Sodium citrate
-
4 mM, 37% inhibition
Zn2+
-
1 mM, 77% inhibition
additional information
-
not inhibitory: 4 mM EDTA or 0.8 mM 2-coumaric acid glucoside
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
stimulation
cysteine
-
stimulation
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6 - 8.3
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-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.8
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25% of maximal activity at pH 6, 10% of maximal activity at pH 9
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
not associated with chloroplasts
-
Manually annotated by BRENDA team
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
1-2 d, complete loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis overnight inactivates
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freezing results in a complete loss of activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, complete loss of activity within 1-2 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE