Information on EC 2.4.1.111 - coniferyl-alcohol glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.111
-
RECOMMENDED NAME
GeneOntology No.
coniferyl-alcohol glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + coniferyl alcohol = UDP + coniferin
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
coniferin metabolism
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monolignol glucosides biosynthesis
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Phenylpropanoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:coniferyl-alcohol 4'-beta-D-glucosyltransferase
Sinapyl alcohol can also act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
61116-23-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
weak activity
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-
Manually annotated by BRENDA team
Ehrh Bark
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
v. Kanrich
-
-
Manually annotated by BRENDA team
weak activity
-
-
Manually annotated by BRENDA team
weak activity
-
-
Manually annotated by BRENDA team
weak activity
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
show the reaction diagram
-
glucosylated at 22% of the activity with coniferyl alcohol
-
-
?
UDP-glucose + caffeic acid
UDP + ?
show the reaction diagram
-
glucosylated at 18% of the activity with coniferyl alcohol
-
-
?
UDP-glucose + coniferyl alcohol
UDP + coniferin
show the reaction diagram
UDP-glucose + coniferyl aldehyde
UDP + coniferaldehyde-4-O-beta-D-glucopyranoside
show the reaction diagram
UDP-glucose + ferulic acid
UDP + ferulic acid-4-O-beta-D-glucopyranoside
show the reaction diagram
UDP-glucose + isorhamnetin
UDP + isorhamnetin 7-O-beta-D-glucopyranoside
show the reaction diagram
UDP-glucose + scopoletin
UDP + scopoletin 7-O-beta-D-glucopyranoside
show the reaction diagram
UDP-glucose + scopoletin
UDP + scopoletin-7-O-beta-D-glucopyranoside
show the reaction diagram
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glucosylated at 10% of the activity with coniferyl alcohol
-
-
?
UDP-glucose + sinapaldehyde
UDP + sinapaldehyde-4-O-beta-D-glucopyranoside
show the reaction diagram
UDP-glucose + sinapic acid
UDP + ?
show the reaction diagram
UDP-glucose + sinapyl alcohol
UDP + syringin
show the reaction diagram
UDP-glucose + vanillic acid
UDP + vanillic acid-4-O-beta-D-glucopyranoside
show the reaction diagram
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glucosylated at 14% of the activity with coniferyl alcohol
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-
?
UDP-glucose + vanillin
UDP + vanillin-4-O-beta-D-glucopyranoside
show the reaction diagram
UDPglucose + coniferyl alcohol
?
show the reaction diagram
additional information
?
-
-
TDPglucose can replace UDPglucose with 17% efficiency
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-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPglucose + coniferyl alcohol
?
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
coniferin
coniferyl alcohol
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above 0.08 mM, Paul's scarlet rose
UDP
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non-competitive, product inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0033 - 0.25
coniferyl alcohol
0.065 - 0.3174
coniferyl aldehyde
0.2417
sinapaldehyde
-
-
0.0056 - 0.58
sinapyl alcohol
0.02 - 0.22
UDPglucose
0.64
Vanillin
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.036
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Paul's scarlet rose
2.436
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 8.2
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pH 5.5: about 50% of maximal activity, pH 8.2: about 70% of maximal activity, phosphate buffer
6.5 - 8.2
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about half-maximal activity at pH 6.5 and 83% of maximal activity at pH 8.2, Tris buffer
6.5 - 8.4
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about half-maximal activity at pH 6.5 and 8.4, Tris buffer
7 - 9.2
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about half-maximal activity at pH 7 and 9.2, phosphate buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 45
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35°C: 96% of maximal activity, 45°C: 83% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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predominantly located in epidermal and subepidermal layers, vascular bundles
Manually annotated by BRENDA team
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segment
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
parietal cytoplasmic layer
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
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gel filtration
50000
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1 * 50000, SDS-PAGE
52000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 50000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
addition of high molarity buffer, 200 mM Tris/HCl, or 2-mercaptoethanol, optimal concentration 42 mM, increases stability considerably
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in dilute solutions addition of 10-20% glycerol or ethylene glycol increases stability
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 200 mM Tris/HCl, pH 7.5, 15% glycerol, stable for several months
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-20°C, addition of 28 mM 2-mercaptoethanol and 10% ethyleneglycol are required for stability, about 20% loss of activity within several weeks
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0-2°C, several weeks
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glycerol, 15%, stabilizes during storage
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE