Information on EC 2.4.1.104 - o-dihydroxycoumarin 7-O-glucosyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.4.1.104
-
RECOMMENDED NAME
GeneOntology No.
o-dihydroxycoumarin 7-O-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + 7,8-dihydroxycoumarin = UDP + daphnin
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
cichoriin interconversion
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-
daphnin interconversion
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-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:7,8-dihydroxycoumarin 7-O-beta-D-glucosyltransferase
Converts the aglycone daphetin into daphnin and, more slowly, esculetin into cichoriin, umbelliferone into skimmin, hydrangetin into hydrangin and scopoletin into scopolin.
CAS REGISTRY NUMBER
COMMENTARY hide
74114-37-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
show the reaction diagram
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-
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-glucose + 4-coumaric acid
UDP + 4-O-glucosylcoumaric acid
show the reaction diagram
-
-
-
-
?
UDP-glucose + 4-methylumbelliferone
UDP + 7-O-glucosyl-4-methylumbelliferone
show the reaction diagram
-
-
-
-
?
UDP-glucose + 7,8-dihydroxycoumarin
UDP + daphnin
show the reaction diagram
UDP-glucose + baicalein
UDP + 7-O-glucosylbaicalein
show the reaction diagram
-
-
-
-
?
UDP-glucose + caffeic acid
UDP + ?
show the reaction diagram
UDP-glucose + esculetin
UDP + cichoriin
show the reaction diagram
UDP-glucose + ferulic acid
UDP + 4-O-glucosylferulic acid
show the reaction diagram
-
low activity
-
-
?
UDP-glucose + formononetin
UDP + 7-O-glucosylformononetin
show the reaction diagram
-
-
-
-
?
UDP-glucose + hydrangetin
UDP + hydrangin
show the reaction diagram
UDP-glucose + protocatechuic acid
UDP + ?
show the reaction diagram
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12% of the activity with 7,8-dihydroxycoumarin
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-
?
UDP-glucose + scopoletin
UDP + scopolin
show the reaction diagram
UDP-glucose + syringic acid
UDP + 4-O-glucosyl syringic acid
show the reaction diagram
-
10% of the activity with 7,8-dihydroxycoumarin
-
-
?
UDP-glucose + umbelliferone
UDP + skimmin
show the reaction diagram
UDP-glucose + vanillic acid
UDP + 4-O-glucosylvanillic acid
show the reaction diagram
-
14% of the activity with 7,8-dihydroxycoumarin
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-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 7,8-dihydroxycoumarin
UDP + daphnin
show the reaction diagram
-
i.e. daphnetin, involved in the glycosylation of daphnetin, esculetin and possibly scopoletin to their corresponding 7-O-glucosides daphnin, chicoriin and scopolin
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?
UDP-glucose + esculetin
UDP + cichoriin
show the reaction diagram
-
i.e. daphnetin, involved in the glycosylation of daphnetin, esculetin and possibly scopoletin to their corresponding 7-O-glucosides daphnin, chicoriin and scopolin
-
?
UDP-glucose + scopoletin
UDP + scopolin
show the reaction diagram
-
i.e. daphnetin, involved in the glycosylation of daphnetin, esculetin and possibly scopoletin to their corresponding 7-O-glucosides daphnin, chicoriin and scopolin
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
1 mM, activation to 114% of control
Cu2+
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1 mM, activation to 112% of control
Mn2+
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1 mM, activation to 133% of control
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
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1 mM, 87% inhibition
Cu2+
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1 mM, 55% inhibition
Zn2+
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1 mM, 96% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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10 mM, activation to 141% of control
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.095
daphnetin
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0.025 - 0.11
esculetin
0.15 - 1.43
scopoletin
0.043 - 0.05
UDPglucose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47000 - 50000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 49000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE