Information on EC 2.4.1.103 - alizarin 2-beta-glucosyltransferase

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The expected taxonomic range for this enzyme is: Streptomyces aureofaciens

EC NUMBER
COMMENTARY hide
2.4.1.103
-
RECOMMENDED NAME
GeneOntology No.
alizarin 2-beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + 1,2-dihydroxy-9,10-anthraquinone = UDP + 1-hydroxy-2-(beta-D-glucosyloxy)-9,10-anthraquinone
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:1,2-dihydroxy-9,10-anthraquinone 2-O-beta-D-glucosyl transferase
Acts on other hydroxy- and dihydroxy-derivatives of 9,10-anthraquinone.
CAS REGISTRY NUMBER
COMMENTARY hide
74506-41-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain Bg, 84-25, NMG-2 and UV61
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 1,4-dihydroxy-9,10-anthraquinone
UDP + 1-hydroxy-4-(beta-D-glucosyloxy)-9,10-anthraquinone
show the reaction diagram
-
7% of the activity with 1,2-dihydroxy-9,10-anthraquinone
-
-
?
UDP-glucose + 1,5-dihydroxy-9,10-anthraquinone
UDP + 1-hydroxy-5-(beta-D-glucosyloxy)-9,10-anthraquinone
show the reaction diagram
-
2% of the activity with 1,2-dihydroxy-9,10-anthraquinone
-
-
?
UDP-glucose + alizarin
UDP + 1-hydroxy-2-(beta-D-glucosyloxy)-9,10-anthraquinone
show the reaction diagram
UDPglucose + 1,3-dihydroxy-9,10-anthraquinone
UDP + 1-hydroxy-3-(beta-D-glucosyloxy)-9,10-anthraquinone
show the reaction diagram
-
48% of the activity with 1,2-dihydroxy-9,10-anthraquinone
-
-
?
UDPglucose + 1,8-dihydroxy-9,10-anthraquinone
UDP + 1-hydroxy-8-(beta-D-glucosyloxy)-9,10-anthraquinone
show the reaction diagram
-
9% of the activity with 1,2-dihydroxy-9,10-anthraquinone
-
-
?
UDPglucose + 1-hydroxy-3-methoxy9,10-anthraquinone
UDP + 3-methoxy-1-(beta-D-glucosyloxy)-9,10-anthraquinone
show the reaction diagram
-
2% of the activity with 1,2-dihydroxy-9,10-anthraquinone
-
-
?
UDPglucose + 1-hydroxy-9,10-anthraquinone
UDP + 1-(beta-D-glucosyloxy)-9,10-anthraquinone
show the reaction diagram
-
3% of the activity with 1,2-dihydroxy-9,10-anthraquinone
-
-
?
UDPglucose + 2,6-dihydroxy-9,10-anthraquinone
UDP + 1-hydroxy-6-(beta-D-glucosyloxy)-9,10-anthraquinone
show the reaction diagram
-
51% of the activity with 1,2-dihydroxy-9,10-anthraquinone
-
-
?
UDPglucose + 2-hydroxy-9,10-anthraquinone
UDP + 2-(beta-D-glucosyloxy)-9,10-anthraquinone
show the reaction diagram
-
32% of the activity with 1,2-dihydroxy-9,10-anthraquinone
-
-
?
UDPglucose + 3-hydroxy-1-methoxy-9,10-anthraquinone
UDP + 1-methoxy-3-(beta-D-glucosyloxy)-9,10-anthraquinone
show the reaction diagram
-
52% of the activity with 1,2-dihydroxy-9,10-anthraquinone
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.11
alizarin
-
pH 7.4, 30°C
0.0108
UDPglucose
-
pH 7.4, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1
-
glucosylation of 1,2-dihydroxy-9,10-anthraquinone
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 7.6
-
about half-maximal activity at pH 6.7 and 7.6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 2 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE