Information on EC 2.4.1.10 - levansucrase

Word Map on EC 2.4.1.10
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.4.1.10
-
RECOMMENDED NAME
GeneOntology No.
levansucrase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
fructan biosynthesis
-
-
Metabolic pathways
-
-
Starch and sucrose metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
sucrose:[6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside 6-beta-D-fructosyltransferase
Some other sugars can act as D-fructosyl acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
9030-17-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain WVU45; strain WVU45, gene ftf
-
-
Manually annotated by BRENDA team
strain WVU45; strain WVU45, gene ftf
-
-
Manually annotated by BRENDA team
Aerobacter levanicum
-
-
-
Manually annotated by BRENDA team
DSM319; strain DSM319, gene sacB, expression in Escherichia coli, 74% identity at the amino acid sequence level with SacB from Bacillus subtilis
-
-
Manually annotated by BRENDA team
strain TH4-2
-
-
Manually annotated by BRENDA team
strain TH4-2
-
-
Manually annotated by BRENDA team
BS5C4 constitutive strain
-
-
Manually annotated by BRENDA team
NCIMB 11871
Uniprot
Manually annotated by BRENDA team
strain NRC 33a
-
-
Manually annotated by BRENDA team
strain NRC33a
-
-
Manually annotated by BRENDA team
strain QB112
-
-
Manually annotated by BRENDA team
strain QB127
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
ATCC12980
-
-
Manually annotated by BRENDA team
strain SRT4, recombinant enzyme expressed in Escherichia coli
UniProt
Manually annotated by BRENDA team
strain 21
-
-
Manually annotated by BRENDA team
strain TMW 1.392
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
isolated from fresh green peas
-
-
Manually annotated by BRENDA team
isoform 6G-FFT2
UniProt
Manually annotated by BRENDA team
KCCM35411
-
-
Manually annotated by BRENDA team
NRRL B-1678
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain S-4380, gene lscA, expression in Escherichia coli DH5alpha
-
-
Manually annotated by BRENDA team
gene lscB; two functional lsc alleles
UniProt
Manually annotated by BRENDA team
gene lscB; two functional lsc alleles
UniProt
Manually annotated by BRENDA team
strain ATCC15552, lscR gene
-
-
Manually annotated by BRENDA team
strain ATCC33071, gene lsrA
SwissProt
Manually annotated by BRENDA team
strain JCM-1683
-
-
Manually annotated by BRENDA team
KCTC2858
-
-
Manually annotated by BRENDA team
ATCC18894
-
-
Manually annotated by BRENDA team
ATCC 13419
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
; var. Homeros
-
-
Manually annotated by BRENDA team
strain ZM1
Q60114
GenBank
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-kestotriose + sucrose
1,6-kestrotetraose + ?
show the reaction diagram
-
1-kestotriose is the preferred substrate
-
-
?
2 sucrose
6-kestose + D-glucose
show the reaction diagram
2 sucrose
D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside
show the reaction diagram
6G-kestotriose + sucrose
6-kestotriose + ?
show the reaction diagram
-
-
-
-
?
alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside
?
show the reaction diagram
alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside
?
show the reaction diagram
alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside
?
show the reaction diagram
D-raffinose
D-fructose + melibiose
show the reaction diagram
-
-
-
?
inulin + H2O
?
show the reaction diagram
raffinose + (2,6-beta-D-fructosyl)n
galactose + (2,6-beta-D-fructosyl)n+1
show the reaction diagram
stachyose + (2,6-beta-D-fructosyl)n
?
show the reaction diagram
-
-
-
-
?
sucrose
levan + kestose
show the reaction diagram
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
show the reaction diagram
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
show the reaction diagram
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
show the reaction diagram
sucrose + ?
blastose + ?
show the reaction diagram
sucrose + ?
polyfructan + ?
show the reaction diagram
sucrose + acarbose
D-glucose + 1'-beta-D-fructofuranosyl alpha-acarbose
show the reaction diagram
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1
show the reaction diagram
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n+1
show the reaction diagram
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside
show the reaction diagram
sucrose + cellobiose
?
show the reaction diagram
-
-
-
-
?
sucrose + cellobiose
beta-D-glucopyranosyl-(1,4)-alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside + D-glucose
show the reaction diagram
-
-
-
?
sucrose + cellobiose
D-glucose + beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
show the reaction diagram
sucrose + D-arabinose
?
show the reaction diagram
-
-
-
-
?
sucrose + D-fucose
beta-D-fructofuranosyl-alpha-D-fucopyranoside + D-glucose
show the reaction diagram
-
-
-
?
sucrose + D-galactose
?
show the reaction diagram
-
-
-
-
?
sucrose + D-galactose
beta-D-fructofuranosyl-alpha-D-galactopyranoside + D-glucose
show the reaction diagram
-
-
-
?
sucrose + D-glucose
glucose + sucrose
show the reaction diagram
sucrose + D-maltose
?
show the reaction diagram
sucrose + D-sorbitol
?
show the reaction diagram
sucrose + D-xylose
?
show the reaction diagram
sucrose + D-xylose
beta-D-fructofuranosyl-alpha-D-xylopyranoside + D-glucose
show the reaction diagram
-
-
-
?
sucrose + D-xylose
beta-D-fructofuranosyl-beta-D-xylopyranoside + D-glucose
show the reaction diagram
-
-
-
?
sucrose + D-xylose
D-glucose + alpha-D-xylopyranosyl-(1->2)-beta-D-fructofuranoside
show the reaction diagram
sucrose + D-xylose
D-glucose + beta-D-fructofuranosyl-(2,1)-alpha-D-xylopyranoside
show the reaction diagram
-
-
NMR product analysis
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
sucrose + hydroquinone
D-glucose + 4-hydroxyphenyl-beta-D-fructofuranoside
show the reaction diagram
sucrose + inositol
?
show the reaction diagram
-
low activity
-
-
?
sucrose + inulin
?
show the reaction diagram
-
-
-
-
?
sucrose + isomaltose
isomaltosyl-fructose + D-glucose
show the reaction diagram
-
-
-
?
sucrose + L-arabinose
?
show the reaction diagram
-
-
-
-
?
sucrose + L-galactose
beta-D-fructofuranosyl-beta-L-galactopyranoside + D-glucose
show the reaction diagram
-
-
-
?
sucrose + L-glucose
beta-D-fructofuranosyl-beta-L-glucopyranoside + D-glucose
show the reaction diagram
-
-
-
?
sucrose + L-xylose
beta-D-fructofuranosyl-beta-L-xylopyranoside + D-glucose
show the reaction diagram
-
-
-
?
sucrose + lactose
?
show the reaction diagram
sucrose + lactose
beta-D-galactopyranosyl-(1,4)-alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside + D-glucose
show the reaction diagram
-
-
-
?
sucrose + lactose
D-glucose + beta-D-fructofuranosyl-beta-D-galactopyranosyl-(1-4)-beta-D-glucopyranoside
show the reaction diagram
-
-
-
-
?
sucrose + lactose
D-glucose + beta-D-galactopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
show the reaction diagram
sucrose + lactose
D-glucose + beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
show the reaction diagram
sucrose + lactose
D-glucose + lactosylfructoside
show the reaction diagram
sucrose + lactose
D-glucose + O-beta-D-galactopyranosyl-(1,4)-O-beta-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
show the reaction diagram
sucrose + lactose
lactosucrose
show the reaction diagram
sucrose + levan
?
show the reaction diagram
mutant enzymes H243L and S164A synthesize either high or low levan molecular weight
-
-
?
sucrose + maltose
?
show the reaction diagram
-
-
-
-
?
sucrose + maltose
D-glucose + alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
show the reaction diagram
sucrose + maltose
erlose + D-glucose
show the reaction diagram
-
-
-
?
sucrose + maltotriose
?
show the reaction diagram
-
-
-
-
?
sucrose + melibiose
?
show the reaction diagram
-
-
-
-
?
sucrose + melibiose
D-glucose + raffinose
show the reaction diagram
-
-
-
?
sucrose + melibiose
raffinose + D-glucose
show the reaction diagram
-
-
-
?
sucrose + methanol
glucose + methyl-beta-D-fructoside
show the reaction diagram
sucrose + raffinose
melibiose + ?
show the reaction diagram
-
-
-
-
?
sucrose + sucrose
?
show the reaction diagram
sucrose + sucrose
D-glucose + (2,6-beta-D-fructosyl)3
show the reaction diagram
Q60114
dimer form
degree of polymerisation 3
-
?
sucrose + sucrose
D-glucose + 1-kestose
show the reaction diagram
sucrose + sucrose
D-glucose + 6-kestose
show the reaction diagram
sucrose + sucrose
D-glucose + levan
show the reaction diagram
-
is assayed in a standard reaction containing 1 ml 10% sucrose and 1 ml enzyme (0.01-0.05mg/ml) incubated at 15C
-
-
?
sucrose + sucrose
D-glucose + neokestose
show the reaction diagram
-
-
identified by one-dimensional and correlation spectroscopy (i.e. COSY, TOCSY, HMBC, DEPT and HSQC)
-
?
sucrose + sucrose
D-glucose + nystose
show the reaction diagram
-
-
identified by HPAEC
-
?
sucrose + sucrose
kestose + D-glucose
show the reaction diagram
-
high concentration of sucrose
-
-
?
sucrose + triisopropylbenzenesulfonyllevan
?
show the reaction diagram
-
-
-
-
?
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside
D-glucose +[6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
show the reaction diagram
Q60114
pH lower than 7.0, fibril form
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 sucrose
D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside
show the reaction diagram
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
show the reaction diagram
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
show the reaction diagram
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
show the reaction diagram
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1
show the reaction diagram
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside
show the reaction diagram
sucrose + lactose
D-glucose + O-beta-D-galactopyranosyl-(1,4)-O-beta-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
show the reaction diagram
-
levansucrase transfers the fructosyl moiety of sucrose to lactose to form lactosucrose
i.e. lactosucrose or 4G-beta-D-galactosylsucrose
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
4fold increase in activity at 5 mM
Fe3+
-
dependent on for tertiary structure, can partially be replaced by Sr2+, not Mg2+, Ba2+, and Mn2+
Mg2+
-
activates both transfructosylation and hydrolytic activities
Mn2+
-
The transferase activity of levansucrase in the reaction mixture supplemented with Mn2+ is 100% higher than the enzyme activity in medium without metal ions, the hydrolytic activity of the levansucrase is lowered by 80%
MnCl2
-
22% activation of the immobilized enzyme at 0.1 mM, 30% activation at 0.1 mM of the free enzyme
Rb+
-
activates slightly, recombinant enzyme
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
slight inhibition at 50 mM
6-phosphogluconate
-
reaction intermediate
AgCl
-
immobilized enzyme: 38% inhibition at 0.1 mM, 67% inhibition at 1 mM, complete inhibition at 10 mM. Free enzyme: 49% inhibition at 0.1 mM, 71% inhibition at 1 mM, 95% inhibition at 10 mM
AgNO3
1 mM, 86% inhibition
CuSO4
-
immobilized enzyme: 25% inhibition at 0.1 mM, 37% inhibition at 1 mM, 65% inhibition at 10 mM. Free enzyme: 30% inhibition at 0.1 mM, 42% inhibition at 1 mM, 79% inhibition at 10 mM
D-glucose
dithiothreitol
ethanol
-
complete inhibition at 1.6 M
Fe3+
-
20 ng/ml medium, 30% inhibition of the free enzyme, 22% inhibition of the immobilized enzyme
FeCl3
-
immobilized enzyme: 8% inhibition at 0.1 mM, 21% inhibition at 1 mM, 35% inhibition at 10 mM. Free enzyme: 13% inhibition at 0.1 mM, 29% inhibition at 1 mM, 40% inhibition at 10 mM
FeSO4
1 mM, 17% inhibition
HgCl2
-
immobilized enzyme: 54% inhibition at 0.1 mM, 61% inhibition at 1 mM, 79% inhibition at 10 mM. Free enzyme: 61% inhibition at 0.1 mM, 75% inhibition at 1 mM, 91% inhibition at 10 mM
High ionic strength
-
Iodine
-
slight inhibition
mannose
Mersalyl acid
-
-
MgSO4
-
11% inhibition of the immobilized enzyme at 10 mM, 21% inhibition of the free enzyme at 10 mM
N-bromosuccinimide
-
-
phenylmethanesulfonyl fluoride
-
-
proteinase K
-
raffinose
SDS
-
immobilized enzyme: 11% inhibition at 0.1 mM, 21% inhibition at 1 mM, 48% inhibition at 10 mM. Free enzyme: 14% inhibition at 0.1 mM, 27% inhibition at 1 mM, 52% inhibition at 10 mM
Sucrose
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
aspartic acid
-
5 mM, activates partially purified enzyme
elugent
-
slight activation at 1%
-
ethanol
-
ethanol increases the fructooligosaccharide biosynthesis activity of levansucrase, fructooligosaccharide production at different temperatures in the presence of ethanol, inverse relationship between temperature and ratio synthetic activity/total activity of levansucrase, overview
fructose
-
slight activation
fructose 1,6-biphosphate
-
5 mM, activates partially purified enzyme
glucoseamine-HCl
-
5 mM, activates partially purified enzyme
levan
Sodium dodecyl sulfate
-
slight activation at 5% w/v
Tween 20
-
slight activation at 1%
additional information
levan has no effect on Y429N, a mutant that has lost the fructan-synthesizing activity
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
47.81
D-glucose
-
pH 6.0, 30C, recombinant enzyme
2.3 - 480.4
Sucrose
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0039 - 3743
Sucrose
additional information
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
39.9 - 80.8
raffinose
2000 - 2800
Sucrose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.1217
-
sucrose, in culture supernatants
0.13
-
glucose, in culture supernatants
2.562
-
glucose, in wild-type strain
3.747
-
sucrose, in wild-type strain
10.8
-
native enzyme from strain type 1, culture supernatant
16.82
-
partially purified free enzyme
37.32
-
purified recombinant His-tagged enzyme from Escherichia coli, release of glucose
50
-
partially purified enzyme from cell wall
90
-
partially purified extracellular enzyme
135.4
-
purified enzyme, pH 6.5, 40C
171
-
levansucrase-ELP[V-40], measured spectrophotometrically
173
-
levansucrase-ELP[V-20], measured spectrophotometrically
195.6
-
purified enzyme
203
-
purified enzyme
230
purified recombinant enzyme
415
-
purified enzyme
461.6
-
purified recombinant wild-type enzyme, pH 6.0, 37C
470
Aerobacter levanicum
-
purified enzyme
593
-
partially purified native enzyme
669.6
-
recombinant His6-tagged enzyme from Bacillus megaterium, culture supernatant
731
-
purified enzyme
791
purified recombinant His-tagged enzyme, pH 6.5, 50C, transfructosylation with lactose as acceptor substrate
2561
-
purified periplasmic enzyme
2603
-
purified extracellular enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 4.6
-
45C, optimal enzymatic activity is observed in this range, the influence of the buffering substance is not signifcant
5.2 - 6
-
free and immobilized enzymes
5.5 - 7
-
hydrolysis rate increases beyond pH 5.0
5.5 - 6
-
recombinant enzyme
5.6
-
immobilized enzyme
5.75
-
assay at
5.8 - 6.6
sucrose hydrolysis
6
Aerobacter levanicum
-
-
6 - 7
-
; wild-type, 50 mM Sorensens phosphate buffer at 37C, containing 500 mM sucrose using an enzyme concentration of 7.36 mg/l
8
-
levan formation, recombinant enzyme
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 7.5
-
50% of the maximal activity are detected at pH 3 and pH 5.5, residual activity is measured at pH 7.5
3 - 10
-
almost no activity under pH 3.0 and above pH 10.0
3.5 - 8.3
pH 3.5: about 55% of maximal activity, pH 8.3: about 60% of maximal activity, levan synthesis
4 - 9
-
pH activity profile, overview
4 - 8.5
-
native and recombinant enzymes, 50% of maximal activity at pH 4.0 and pH 8.5
4 - 8
-
activity range, free and immobilized enzymes
4 - 6.2
pH 4.0: about 50% of the optimal activity, pH 6.2: about 50% of maximal activity. A pH above 7 leads to a complete inhibition of the levansucrase
4 - 6
-
activity range, no activity below pH 3.5 and above pH 6.5, profile, overview
4.4 - 6.8
-
activity range of free and immobilized enzymes, with higher activity of the immobilized enzyme, profiles, overview
4.8 - 6.8
-
about half-maximal activity at pH 4.8 and pH 6.8
5 - 8.4
Q60114
recombinant enzyme
5.5 - 8
-
activity range, profile overview
5.6 - 7.6
-
the enzyme activity significantly decreases at pH values below 5.6 and above 7.6; wild-type, 50 mM Sorensens phosphate buffer at 37C, containing 500 mM sucrose using an enzyme concentration of 7.36 mg/l
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
levan formation
4 - 40
-
assay at, levan formation reaches the highest value at 4C and 100 g/l sucrose. The increase of temperature to 40C and fructose concentration of 600 g/l causes a significant decrease of the levan concentration and a higher production of short-chain fructooligosaccharides, an increase of the temperature also causes an enhancement of hydrolytic activity
8 - 30
assays at 8C and 30C
22
-
assay at
27
-
assay at
35 - 40
45 - 50
-
sodium acetate pH 4.1 containing 5 mM CaCl2
55
-
in 0.38 M phosphate buffer
55 - 60
-
sucrose + D-xylose
additional information
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 30
-
inactive above
15 - 50
-
activity range, free and immobilized enzymes
20 - 60
-
native and recombinant enzymes lose 80% activities at temperatures below 20C and are nearly inactive at above 60C
25 - 50
30 - 40
-
30C: maximal activity, 40C: 21% of maximal activity
30 - 65
-
activity range, profile overview
35 - 45
-
fructooligosaccharide production at different temperatures in the presence of ethanol, overview
40
-
50% activity is reached at 40C
50
-
above 50C a sharp loss of activity is observed
60
-
at 60C practically no activity is detectable
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.7
calculated from sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
enzyme production is performed by free and differently immobilized cells, maximum yield with 18.47 U/ml is achieved after 48 h in batch shaken culture of the free cells, but immobilized culture technique allows the achievement of relatively high enzyme activities of 5.5-10.75 U/ml with successful repeated utilization of the immobilized cells
Manually annotated by BRENDA team
-
; developing from anthesis until maturity. 1,6-Kestotetraose formation is prominent during the first 13 days after anthesis, and little or no 6-SFT activity is observed later on during maturation
Manually annotated by BRENDA team
the relative expression of the fructan biosynthetic genes 1-SST and 6-SFT, encoding sucrose:sucrose 1-fructosyltransferase and sucrose:fructan 6-fructosyltransferase, respectively, and 6G-FFT/1-FFT isoform 6G-FFT 2 correlates positively with the accumulation of fructans in both blades and pseudostems. Isoform 6G-FFT 2 is highly expressed in cultivar E, that accumulatss the highest levels of fructans in this study
Manually annotated by BRENDA team
-
expression of the levan-forming enzyme, levansucrase, occurrs mainly during early exponential growth of both planktonic and sessile cells
Manually annotated by BRENDA team
the relative expression of the fructan biosynthetic genes 1-SST and 6-SFT, encoding sucrose:sucrose 1-fructosyltransferase and sucrose:fructan 6-fructosyltransferase, respectively, and 6G-FFT/1-FFT isoform 6G-FFT 2 correlates positively with the accumulation of fructans in both blades and pseudostems. Isoform 6G-FFT 2 is highly expressed in cultivar E, that accumulatss the highest levels of fructans in this study
Manually annotated by BRENDA team
-
expression of the levan-forming enzyme, levansucrase, occurrs mainly during early exponential growth of both planktonic and sessile cells
Manually annotated by BRENDA team