Information on EC 2.3.3.4 - decylhomocitrate synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.3.4
-
RECOMMENDED NAME
GeneOntology No.
decylhomocitrate synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dodecanoyl-CoA + H2O + 2-oxoglutarate = (3S,4S)-3-hydroxytetradecane-1,3,4-tricarboxylate + CoA
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
condensation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
dodecanoyl-CoA:2-oxoglutarate C-dodecanoyltransferase (thioester-hydrolysing, 1-carboxyundecyl-forming)
Decanoyl-CoA can act instead of dodecanoyl-CoA, but 2-oxoglutarate cannot be replaced by oxaloacetate or pyruvate.
CAS REGISTRY NUMBER
COMMENTARY hide
51845-40-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Penicillium spiculisporum
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
decanoyl-CoA + H2O + 2-oxoglutarate
(3S,4S)-3-hydroxydodecane-1,3,4-tricarboxylate + CoA
show the reaction diagram
Penicillium spiculisporum
-
-
-
-
?
dodecanoyl-CoA + H2O + 2-oxoglutarate
(3S,4S)-3-hydroxytetradecane-1,3,4-tricarboxylate + CoA
show the reaction diagram
Penicillium spiculisporum
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dodecanoyl-CoA + H2O + 2-oxoglutarate
(3S,4S)-3-hydroxytetradecane-1,3,4-tricarboxylate + CoA
show the reaction diagram
Penicillium spiculisporum
-
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoic acid)
Penicillium spiculisporum
-
enzyme inactivation, lauroyl-CoA or palmitoyl-CoA protects
oxaloacetate
Penicillium spiculisporum
-
competitive to 2-oxoglutarate
palmitoyl-CoA
Penicillium spiculisporum
-
partially competitive with lauroyl-CoA
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.75
2-oxoglutarate
Penicillium spiculisporum
-
-
0.001
Lauroyl-CoA
Penicillium spiculisporum
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4 - 0.8
oxaloacetate
Penicillium spiculisporum
-
competitive inhibitor
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.25
Penicillium spiculisporum
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.3
Penicillium spiculisporum
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3 - 9
Penicillium spiculisporum
-
75% of maximal activity at pH 7.5 and 9
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
Penicillium spiculisporum
-
? * 43000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
Penicillium spiculisporum
-