Information on EC 2.3.2.B1 - lipid II:alanine alanyl transferase

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The expected taxonomic range for this enzyme is: Streptococcus pneumoniae

EC NUMBER
COMMENTARY hide
2.3.2.B1
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
lipid II:alanine alanyl transferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA = UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine:alanine N6-alanyltransferase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
penicillin-resistant strain
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Manually annotated by BRENDA team
penicillin-resistant strain
UniProt
Manually annotated by BRENDA team
penicillin-susceptible strain
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-
Manually annotated by BRENDA team
penicillin-susceptible strain
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ditrans,ocatacis-undecaprenyl diphosphoryl (GlcNAc-beta-(1->4))MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-Ala-tRNa
ditrans,ocatacis-undecaprenyl diphosphoryl (GlcNAc-beta-(1->4))MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA
show the reaction diagram
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-
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-
?
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA
? + tRNA
show the reaction diagram
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine + alanyl-tRNA
? + tRNA
show the reaction diagram
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA
show the reaction diagram
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-seryl-tRNA
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + tRNA
show the reaction diagram
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2'-(1-amino ethyl phosphonyl) adenosine
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1 mM, 55% inhibition
3'-(1-amino ethyl phosphonyl) 2'-deoxyadenosine
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1 mM, 95% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.037
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
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pH 7.6, 37C
0.031
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine
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pH 7.6, 37C
0.00021
L-alanyl-tRNA
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pH 7.6, 37C
0.237
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine
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pH 7.6, 37C
0.035
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
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pH 7.6, 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.017
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
Streptococcus pneumoniae
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pH 7.6, 37C
0.0157
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine
Streptococcus pneumoniae
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pH 7.6, 37C
0.021
L-alanyl-tRNA
Streptococcus pneumoniae
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pH 7.6, 37C
0.0031
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine
Streptococcus pneumoniae
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pH 7.6, 37C
0.033
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
Streptococcus pneumoniae
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pH 7.6, 37C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.205
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
Streptococcus pneumoniae
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pH 7.6, 37C
41326
0.46
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine
Streptococcus pneumoniae
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pH 7.6, 37C
41327
0.1
L-alanyl-tRNA
Streptococcus pneumoniae
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pH 7.6, 37C
4913
0.013
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine
Streptococcus pneumoniae
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pH 7.6, 37C
7911
0.948
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
Streptococcus pneumoniae
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pH 7.6, 37C
19387
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.78
2'-(1-amino ethyl phosphonyl) adenosine
Streptococcus pneumoniae
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pH 6.8, 37C
0.1
3'-(1-amino ethyl phosphonyl) 2'-deoxyadenosine
Streptococcus pneumoniae
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pH 6.8, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.172
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substrate L-alanyl-tRNA from penicillin-susceptible strain Pn16, pH 7.6, 37C
0.175
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substrate L-alanyl-tRNA from penicillin-resistant strain 159, pH 7.6, 37C
0.247
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substrate L-seryl-tRNA from penicillin-resistant strain 159, pH 7.6, 37C
0.28
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substrate L-seryl-tRNA from penicillin-resistant strain 159, pH 7.6, 37C
0.31
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substrate L-seryl-tRNA from penicillin-susceptible strain Pn16, pH 7.6, 37C
0.37
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substrate L-seryl-tRNA from penicillin-susceptible strain Pn16, pH 7.6, 37C
1.92
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substrate L-alanyl-tRNA from penicillin-resistant strain 159, pH 7.6, 37C
2.09
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substrate L-alanyl-tRNA from penicillin-susceptible strain Pn16, pH 7.6, 37C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-tagged recombinant protein
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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