Information on EC 2.3.2.7 - aspartyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.2.7
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RECOMMENDED NAME
GeneOntology No.
aspartyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-asparagine + hydroxylamine = NH3 + beta-L-aspartylhydroxamate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aminoacyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
L-asparagine:hydroxylamine gamma-aspartyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
37257-23-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
H37Ra, strain number 7417
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Manually annotated by BRENDA team
pv. phaseolicola 1448A
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
asparagine + hydroxylamine
NH3 + beta-aspartohydroxamic acid
show the reaction diagram
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strict specificity, L- and D-enantiomer equally effective, no substrate: L-glutamine
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?
L-asparagine + hydroxylamine
NH3 + beta-L-aspartohydroxamic acid
show the reaction diagram
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involved in initial step of asparagine metabolism
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-
?
L-asparagine + hydroxylamine
NH3 + beta-L-aspartylhydroxamate
show the reaction diagram
additional information
?
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no activity with glycylglycine, ethylamine, and methylamine
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-asparagine + hydroxylamine
NH3 + beta-L-aspartohydroxamic acid
show the reaction diagram
-
involved in initial step of asparagine metabolism
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-
?
L-asparagine + hydroxylamine
NH3 + beta-L-aspartylhydroxamate
show the reaction diagram
-
-
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no metal ion requirement
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
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slight inhibition at 5 mM
EDTA
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slight inhibition at 5 mM
L-aspartate
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20 mM, 30% inhibition
L-cysteine
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5 mM, 50% inhibition
PCMB
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reversible by GSH, 2-mercaptoethanol or 2,3-dimercaptopropanol, not L-cysteine
streptomycin
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5 mM, 50% inhibition
additional information
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no inhibition by NaN3, isonicotinic acid hydrazide, L-homoserine, L-glutamic acid, terminal amino acids of aspartic acid pathway, i.e. lysine, threonine, methionine and isoleucine, up to 20 mM
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Isonicotinic acid hydrazide
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activation
L-cystine
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20 mM, slight activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
19.3
hydroxylamine
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7.14
L-asparagine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 11
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more than 40% activity between pH 6.0 and 11.0
8.5 - 9.4
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about half-maximal activity at pH 8.5 and 9.4
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 60
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more than 40% activity between 30 and 60°C
40 - 60
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40°C: about 50% of maximal activity, 60°C: about 80% of maximal activity
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 11
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after 10 min incubation at pH 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, and 11.0, the enzyme shows 30, 20, 55, 100, 95, 80, and 60% residual activity, respectively
735832
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
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6 h, 23% loss of activity
30 - 70
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after 10 min incubation at 30, 50, 50, 60 and 70°C, the enzyme shows 100, 95, 90, 85, and 60% residual activity, respectively
37
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6 h, about 8% loss of activity
60
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10 min, about 45% loss of activity
65
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10 min, about 66% loss of activity
70
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t1/2: 1 min, about 55% loss of activity after 2 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis against 0.05 M Tris buffer, pH 7.0, 75% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-Cellufine column chromatography, butyl Toyopearl column chromatography, and Hitrap CM column chromatography
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