Information on EC 2.3.2.25 - N-terminal E2 ubiquitin-conjugating enzyme

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The expected taxonomic range for this enzyme is: Homo sapiens

EC NUMBER
COMMENTARY hide
2.3.2.25
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RECOMMENDED NAME
GeneOntology No.
N-terminal E2 ubiquitin-conjugating enzyme
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid = [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein]
show the reaction diagram
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [N-terminal E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[N-terminal ubiquitin-conjugating enzyme]-L-cysteine
show the reaction diagram
(1a)
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S-ubiquitinyl-[N-terminal E2 ubiquitin-conjugating E2 enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid = [N-terminal E2 ubiquitin-conjugating enzyme]-L-cysteine + N-ubiquitinyl-[acceptor protein]-N-terminal amino acid
show the reaction diagram
(1b)
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SYSTEMATIC NAME
IUBMB Comments
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine:acceptor protein ubiquitin ligase (peptide bond-forming)
The enzyme ubiquitinylates the N-terminus of the acceptor protein. It is not reactive towards free lysine.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
Ube2W is an E2 ubiquitin-conjugating enzyme with specific protein N-terminal monoubiquitylation activity. Ube2W conjugates ubiquitin to its own N-terminus, but also to that of the small ubiquitin-like modifier SUMO in a manner dependent on the SUMO-targeted ubiquitin ligase RNF4, i.e.RING finger protein 4
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [SUMO-2]-N-terminal-amino acid
[E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[SUMO-2]
show the reaction diagram
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N-terminal mono-ubiquitylation of SUMO-2 primes it for poly-ubiquitylation by the Ubc13-UEV1 (ubiquitin-conjugating enzyme E2 variant 1) heterodimer
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?
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [Ube2W]-N-terminal-amino acid
[E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[Ube2W]
show the reaction diagram
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isoform Ube2W ubiquitylates its own N-terminus
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?
S-ubiquitinyl-[N-terminal E2 ubiquitin-conjugating E2 enzyme]-L-cysteine + [CARP2 protein]-N-terminal-amino acid
[N-terminal E2 ubiquitin-conjugating enzyme]-L-cysteine + N-ubiquitinyl-[ CARP2 protein]-N-terminal amino acid
show the reaction diagram
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-
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?
S-ubiquitinyl-[N-terminal E2 ubiquitin-conjugating E2 enzyme]-L-cysteine + [cIAP2 protein]-N-terminal-amino acid
[N-terminal E2 ubiquitin-conjugating enzyme]-L-cysteine + N-ubiquitinyl-[ cIAP2 protein]-N-terminal amino acid
show the reaction diagram
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-
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?
S-ubiquitinyl-[N-terminal E2 ubiquitin-conjugating E2 enzyme]-L-cysteine + [MDM2 protein]-N-terminal-amino acid
[N-terminal E2 ubiquitin-conjugating enzyme]-L-cysteine + N-ubiquitinyl-[ MDM2 protein]-N-terminal amino acid
show the reaction diagram
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-
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?
[ubiquitin-carrier protein E2]-S-ubiquitinyl-L-cysteine + [ataxin-3]-NH2
[ubiquitin-carrier protein E2]-L-cysteine + N-terminal-ubiquitinyl-[ataxin-3]
show the reaction diagram
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?
[ubiquitin-carrier protein E2]-S-ubiquitinyl-L-cysteine + [protein-tau]-NH2
[ubiquitin-carrier protein E2]-L-cysteine + N-terminal-ubiquitinyl-[protein-tau]
show the reaction diagram
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?
[ubiquitin-carrier protein Ubc16]-S-ubiquitinyl-L-cysteine + [CHIP]-NH2
[ubiquitin-carrier protein Ubc16]-L-cysteine + N-terminal-ubiquitinyl-[CHIP]
show the reaction diagram
CHIP is a U-box E3 ubiquitin ligase known to interact productively with many E2 enzymes
isoform Ubc16 N-terminally mono-ubiquitinates the ubiquitin E3 ligase CHIP
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?
[ubiquitin-carrier protein Ubc16]-S-ubiquitinyl-L-cysteine + [SUMO-2]-NH2
[ubiquitin-carrier protein Ubc16]-L-cysteine + N-terminal-ubiquitinyl-[SUMO-2]
show the reaction diagram
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isoform Ubc16 shows specific protein N-terminal monoubiquitylation activity. Ubc16 conjugates ubiquitin not only to its own N-terminus, but also to that of the small ubiquitin-like modifier SUMO in a manner dependent on the SUMO-targeted ubiquitin ligase RNF4, i.e. RING finger protein 4. N-terminal mono-ubiquitylation of SUMO-2 primes it for poly-ubiquitylation by the Ubc13-UEV1 ubiquitin-conjugating enzyme E2 variant 1 heterodimer
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?
additional information
?
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Ube2w is not reactive toward free lysine and contains novel residues in its active site that are important for activity
to confirm N-terminal ubiquitination lysine-less and N-terminally blocked substrates are generated. The lysine-less substrate is ubiquinated, but not the N-terminally blocked one
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22400
1 * 22400, calculated from amino acid sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 22400, calculated from amino acid sequence
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE