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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
femxwv, alanyltransferase,
more
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alanyl-transfer ribonucleate-uridine diphosphoacetylmuramoylpentapeptide transferase
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alanyltransferase, uridine diphosphoacetylmuramoylpentapeptide lysine N6-
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UDP-N-acetylmuramoylpentapeptide lysine N6-alanyltransferase
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uridine diphosphoacetylmuramoylpentapeptide lysine N6-alanyltransferase
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FemX
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
also acts on L-seryl-tRNA
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L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
transfer of L-Ala to epsilon-amino group of L-lysine of UDP-UDP-acetylmuramoylpentapeptide in an ordered sequential mechanism
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L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
catalysis via a ternary complex
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
aminoacyl group transfer
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aminoacyl group transfer
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-alanyl-tRNAAla:UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine N6-alanyltransferase
Also acts on L-seryl-tRNASer.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
glycyl-tRNAGly + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAGly + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(glycyl)-L-lysyl-D-alanyl-D-alanine
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poor substrate
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ir
L-alanyl-tRNA(Ala) + UDP-N-acetylmuramoyl-pentapeptide containing L-lysine
?
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r
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
L-alanyl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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?
L-cysteinyl-tRNACys + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNACys + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-cysteinyl)-L-lysyl-D-alanyl-D-alanine
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ir
L-seryl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-seryl)-L-lysyl-D-alanyl-D-alanine
additional information
?
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L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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poor or no acceptors are acetylmuramoyl- and phosphoacetylmuramoyl-L-Ala-L-Glu-L-Lys-D-Ala-D-Ala or UDP-acetylmuramoyl-L-Ala-L-Glu-L-Lys
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ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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no strict specificity for tRNA-carrier: tRNACys can replace tRNAAla, tRNA from other species can substitute to some extent for the homologous tRNA from Lactobacillus viridescens, higher specificity for transferred amino acid: amino acids with larger substituents are no substrates, UDP-acetylmuramyl-L-Ala-D-Glu-meso-diaminopimelic acid-D-Ala-D-Ala can replace UDP-acetylmuramyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala at 15% the transfer rate
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ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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involved in peptidoglucan metabolism, responsible for interpeptide bridges
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ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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production of precursors for branched chain peptidoglycans
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?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
production of precursors for branched chain peptidoglycans, involved in the synthesis of bacterial cell walls
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?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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production of precursors for branched chain peptidoglycans
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?
L-seryl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-seryl)-L-lysyl-D-alanyl-D-alanine
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?
L-seryl-tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNASer + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-seryl)-L-lysyl-D-alanyl-D-alanine
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about half as effective as L-alanyl-tRNA
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ir
additional information
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also utilizes UDP-N-acetylmuramoylpentapeptide containing L-ornithine as substrate
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additional information
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enzyme highly specific for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine, only traces of activity with UDP-N-acetylmuramoyl-tripeptide or UDP-N-acetylmuramoyl-tetrapeptide or derivatives of UDP-N-acetylmuramoyl-pentapeptide
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?
additional information
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catalyses the transfer of L-Ala from Ala-tRNAAla to the peptidoglycan precursor UDP-MurNAc-pentapeptide in order to introduce the first residue of an L-Ala-L-Ser-L-Ala side chain
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additional information
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FemXWv transferase adds the first residue of the L-Ala-L-Ser-L-Ala side chain onto the nucleotide precursor UDP-MurNAc-pentadepsipeptide
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?
additional information
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enzyme highly specific for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine, only traces of activity with UDP-N-acetylmuramoyl-tripeptide or UDP-N-acetylmuramoyl-tetrapeptide or derivatives of UDP-N-acetylmuramoyl-pentapeptide
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?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-alanyl-tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
additional information
?
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L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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involved in peptidoglucan metabolism, responsible for interpeptide bridges
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ir
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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production of precursors for branched chain peptidoglycans
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?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
production of precursors for branched chain peptidoglycans, involved in the synthesis of bacterial cell walls
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?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
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production of precursors for branched chain peptidoglycans
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?
additional information
?
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catalyses the transfer of L-Ala from Ala-tRNAAla to the peptidoglycan precursor UDP-MurNAc-pentapeptide in order to introduce the first residue of an L-Ala-L-Ser-L-Ala side chain
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additional information
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FemXWv transferase adds the first residue of the L-Ala-L-Ser-L-Ala side chain onto the nucleotide precursor UDP-MurNAc-pentadepsipeptide
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?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
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no activation by EDTA or metal ions
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aminoacyl-tRNA analogue C
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aminoacyl-tRNA analogue C'
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Ser-tRNA
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versus UDP-acetylmuramoylpentapeptide
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
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product inhibition
additional information
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no inhibition by EDTA or metal ions
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0017
L-alanyl-tRNA
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pH 7.5, 30°C
0.015 - 0.061
L-alanyl-tRNA(Ala)
0.0002 - 0.165
UDP-acetylmuramoylpentapeptide
0.079
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
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pH 7.5, 30°C
0.015
L-alanyl-tRNA(Ala)
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Ala-tRNAAla from E. coli, recombinant wild-type
0.054
L-alanyl-tRNA(Ala)
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Ala-tRNAAla from E. coli, recombinant mutant E320Q
0.061
L-alanyl-tRNA(Ala)
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Ala-tRNAAla from E. coli, recombinant mutant D109N
0.0002
UDP-acetylmuramoylpentapeptide
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0.042
UDP-acetylmuramoylpentapeptide
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recombinant wild-type
0.067
UDP-acetylmuramoylpentapeptide
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recombinant mutant E320Q
0.165
UDP-acetylmuramoylpentapeptide
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recombinant mutant D109N
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
26.7
L-alanyl-tRNA
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pH 7.5, 30°C
0.183 - 11
L-alanyl-tRNAAla
0.183
L-alanyl-tRNAAla
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recombinant mutant D109N
0.917
L-alanyl-tRNAAla
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recombinant mutant E320Q
11
L-alanyl-tRNAAla
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recombinant wild-type
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.248
Ser-tRNA
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versus UDP-acetylmuramoylpentapeptide
0.048
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
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product inhibition
additional information
additional information
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0024
aminoacyl-tRNA analogue C
Weissella viridescens
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0.0041
aminoacyl-tRNA analogue C'
Weissella viridescens
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0.0014
oxa-helixAla
Weissella viridescens
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0.067
oxa-helixGly
Weissella viridescens
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0.015
oxa-helixSer
Weissella viridescens
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
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relative activity of FemXWv for the transfer of L-Ala from helixAla to UDP-MurNAc-pentapeptide 100%
additional information
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relative activity of FemXWv for the transfer of L-Ala from helixGly to UDP-MurNAc-pentapeptide 2.9%
additional information
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relative activity of FemXWv for the transfer of L-Ala from helixSer to UDP-MurNAc-pentapeptide 31%
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6.8 - 7.2
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L-alanyl-tRNA
additional information
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broad maximum
7.5
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inhibition assay
7.5
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radioactive coupled FemX assay
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.2 - 8.6
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L-alanyl-tRNA, about 80% of maximal activity at pH 5.2 and pH 8.6
6.1 - 8.6
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L-seryl-tRNA, about half-maximal activity at pH 6.1 and about 90% of maximal activity at pH 8.6
additional information
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pK values of 5.5 and 9.3
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30
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assay at
30
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radioactive coupled FemX assay
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
formerly Lactobacillus viridescens
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brenda
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brenda
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SwissProt
brenda
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brenda
femX gene
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brenda
formerly Lactobacillus viridescens
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brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
FEMX_WEIVI
336
0
38284
Swiss-Prot
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A0A139NAM5_9STRE
147
0
17134
TrEMBL
-
A0A8B6IYH2_STRPY
407
0
47296
TrEMBL
-
A0A3S4PYH0_9STRE
404
0
46389
TrEMBL
-
E8KUV0_STRVE
404
0
46231
TrEMBL
-
A0A344UQD6_9ACTN
388
0
43684
TrEMBL
-
C5WGD1_STRDG
Streptococcus dysgalactiae subsp. equisimilis (strain GGS_124)
407
0
46634
TrEMBL
-
A0A4V0BXY1_STRCB
407
0
47196
TrEMBL
-
A0A380LL55_9FIRM
411
0
48524
TrEMBL
-
A0A2H5ZMU4_9BACT
342
0
38412
TrEMBL
-
M4YWQ5_STREQ
407
0
46634
TrEMBL
-
A0A7W8D1Q4_9FIRM
407
0
47712
TrEMBL
-
A0A2X2LHM9_STRAG
406
0
46642
TrEMBL
-
F0IN95_STRSA
288
0
33115
TrEMBL
-
A0A380KNF1_9STRE
405
0
46462
TrEMBL
-
C5WFH4_STRDG
Streptococcus dysgalactiae subsp. equisimilis (strain GGS_124)
410
0
46785
TrEMBL
-
F0IU39_STRSA
404
0
46269
TrEMBL
-
A0A8B6J008_STRPY
408
0
46036
TrEMBL
-
A0A380JFD5_STRDO
404
0
46609
TrEMBL
-
F5WVQ4_STRG1
Streptococcus gallolyticus (strain ATCC 43143 / F-1867)
404
1
46424
TrEMBL
-
A9QSG8_LACLK
Lactococcus lactis subsp. lactis (strain KF147)
399
0
46477
TrEMBL
-
A0A8H2RGW0_STRPY
407
0
47232
TrEMBL
-
A0A2S6X7T9_9ACTN
373
0
42478
TrEMBL
-
A0A380I6V2_STRAG
406
0
46826
TrEMBL
-
A0A139R5Z4_9STRE
94
0
11032
TrEMBL
-
Q1J6G4_STRPF
Streptococcus pyogenes serotype M4 (strain MGAS10750)
407
0
47279
TrEMBL
-
A0A399E7Y6_9DEIN
340
0
38407
TrEMBL
-
A0A239SRV1_9STRE
406
0
46510
TrEMBL
-
Q1JLL0_STRPC
Streptococcus pyogenes serotype M12 (strain MGAS9429)
407
0
47292
TrEMBL
-
A0A2X3WB34_9STRE
404
0
46347
TrEMBL
-
X5NYE7_ENTFL
403
0
46252
TrEMBL
-
Q1JMR0_STRPC
Streptococcus pyogenes serotype M12 (strain MGAS9429)
413
0
46559
TrEMBL
-
Q48TF5_STRPM
Streptococcus pyogenes serotype M28 (strain MGAS6180)
407
0
47341
TrEMBL
-
Q1JHV3_STRPD
Streptococcus pyogenes serotype M2 (strain MGAS10270)
413
0
46521
TrEMBL
-
A0A8H2N3C0_STRPY
408
0
45977
TrEMBL
-
A0A4U9XUC0_STRPO
419
0
48699
TrEMBL
-
D4JF17_9FIRM
404
0
47580
TrEMBL
-
A0A2I8AJR4_9STRE
408
0
46637
TrEMBL
-
A0A483BDZ9_OENOE
339
0
39331
TrEMBL
-
F0FE44_STRSA
404
0
46367
TrEMBL
-
Q02W53_LACLS
Lactococcus lactis subsp. cremoris (strain SK11)
396
0
46224
TrEMBL
-
A0A1Q8EFH9_STRAI
405
0
46461
TrEMBL
-
F0I8F0_STRSA
404
0
46209
TrEMBL
-
F3UYI1_STRSA
404
0
46214
TrEMBL
-
Q1JGP5_STRPD
Streptococcus pyogenes serotype M2 (strain MGAS10270)
407
0
47336
TrEMBL
-
A0A8A0RK29_9FIRM
362
0
42200
TrEMBL
-
A0A644ZCE1_9ZZZZ
383
0
42911
TrEMBL
other Location (Reliability: 5 )
A0A288Q7I0_9LACO
338
0
38134
TrEMBL
-
A0A380IQ37_STRAG
406
0
46721
TrEMBL
-
F3UBI0_STRSA
404
0
46331
TrEMBL
-
A0A4U9ZKG6_9STRE
423
0
48903
TrEMBL
-
A0A4V6L7P8_9STRE
413
0
47502
TrEMBL
-
F0I0M2_STRSA
404
0
46225
TrEMBL
-
F0FSH0_STRSA
404
0
46243
TrEMBL
-
A0A4V6LXT6_STRPO
419
0
48699
TrEMBL
-
A0A7W8FW43_9FIRM
410
0
48243
TrEMBL
-
A0A399EJQ9_9DEIN
340
0
38365
TrEMBL
-
J1GYZ7_9STRE
256
0
29917
TrEMBL
-
A0A4V0GYZ1_STRPO
413
0
48011
TrEMBL
-
F2C6R3_STRSA
404
0
46287
TrEMBL
-
F3URF8_STRSA
404
0
46173
TrEMBL
-
Q48UK2_STRPM
Streptococcus pyogenes serotype M28 (strain MGAS6180)
413
0
46692
TrEMBL
-
A0A399EJD3_9DEIN
340
0
37867
TrEMBL
-
A0A4U9XHI3_9STRE
417
0
48406
TrEMBL
-
F2CDZ2_STRSA
404
0
46267
TrEMBL
-
A0A841CAK1_9LACT
401
0
45677
TrEMBL
-
A0A2I8AI09_9STRE
406
0
46399
TrEMBL
-
A0A6I5ZPQ1_9THEO
362
0
41790
TrEMBL
-
F5X2D3_STRPX
Streptococcus pasteurianus (strain ATCC 43144 / JCM 5346 / CDC 1723-81)
404
0
46287
TrEMBL
-
G6ENS4_STRTR
404
0
46210
TrEMBL
-
E8JSL6_STRCR
404
0
46086
TrEMBL
-
E8KCU6_9STRE
428
0
49712
TrEMBL
-
J7T771_STRSL
404
0
46267
TrEMBL
-
A0A380LLJ5_9FIRM
400
0
46688
TrEMBL
-
F3SIH2_STRSA
404
0
46184
TrEMBL
-
A0A2P7ZZB0_9ACTN
397
0
43977
TrEMBL
-
A0A2X3VCH4_9STRE
351
0
40279
TrEMBL
-
A0A101RUC5_9ACTN
373
0
42981
TrEMBL
-
A0A081JG95_STRMC
404
1
46331
TrEMBL
-
A0A380K2B5_9STRE
404
1
46331
TrEMBL
-
F2BSP2_STRSA
404
0
46252
TrEMBL
-
A0A387HLK3_9ACTN
372
0
42717
TrEMBL
-
Q1J7N1_STRPF
Streptococcus pyogenes serotype M4 (strain MGAS10750)
413
0
46565
TrEMBL
-
Q5XC19_STRP6
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
407
0
47307
TrEMBL
-
A0A4U9XHZ0_9STRE
413
0
47515
TrEMBL
-
A0A139NJL6_9STRE
399
0
45198
TrEMBL
-
A0A645JHE0_9ZZZZ
163
0
19199
TrEMBL
other Location (Reliability: 1 )
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38000
crystal structure analysis
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monomer
1 * 38000, crystal structure analysis
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crystallized by vapor diffusion method
crystallized in the presence of UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine by vapor diffusion method
-
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D109N
-
site-directed mutagenesis, loss of 99% activity compared to the wild-type
E205Q
-
site-directed mutagenesis, unaltered activity compared to the wild-type
E215Q
-
site-directed mutagenesis, loss of 15% activity compared to the wild-type
E316Q
-
site-directed mutagenesis, loss of 60% activity compared to the wild-type
E320Q
-
site-directed mutagenesis, loss of 96% activity compared to the wild-type
F70L
-
35% loss of activity
K36M
-
activity complete abolished
K36R
-
160-fold reduction of activity, 5.4-fold increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
R211K
-
17000-fold reduction of activity, no effect on Km for Ala-tRNA, Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine higher than the highest sample concentration
Y103F
-
no effect on activity
Y215F
-
25-fold decrease of activity, increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
Y215L
-
insoluble protein
Y256F
-
2.6-fold reduction of activity
F70L
-
35% loss of activity
-
K36M
-
activity complete abolished
-
R211K
-
17000-fold reduction of activity, no effect on Km for Ala-tRNA, Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine higher than the highest sample concentration
-
Y103F
-
no effect on activity
-
Y215F
-
25-fold decrease of activity, increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
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recombinant His-tagged wild-type and mutants from E. coli
-
recombinant proteins using His-tag
-
-
-
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expressed as intein-tagged fusion protein
expression of wild-type and mutants as His-tagged proteins in Escherichia coli, in vitro transcription of tRNAs
-
wild type and mutant enzymes expressed as His-tag fusion proteins in Escherichia coli TOP10
-
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medicine
potential target for antimicrobial drugs
medicine
-
Fem transferases are considered as attractive targets for the development of novel antibiotics active against multi-resistant bacteria
medicine
-
Fem transferases are considered attractive targets for the development of novel antibiotics active against multiply resistant bacteria
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Plapp, R.; Strominger, J.L.
Biosynthesis of the peptidoglycan of bacterial cell walls
J. Biol. Chem.
245
3673-3682
1970
Weissella viridescens
-
brenda
Hegde, S.S.; Blanchard, J.S.
Kinetic and mechanistic characterization of recombinant Lactobacillus viridescens FemX (UDP-N-acetylmuramoyl pentapeptide-lysine N6-alanyltransferase)
J. Biol. Chem.
278
22861-22867
2003
Weissella viridescens
brenda
Maillard, A.P.; Biarrotte-Sorin, S.; Villet, R.; Mesnage, S.; Bouhss, A.; Sougakoff, W.; Mayer, C.; Arthur, M.
Structure-based site-directed mutagenesis of the UDP-MurNAc-pentapeptide-binding cavity of the FemX alanyl transferase from Weissella viridescens
J. Bacteriol.
187
3833-3838
2005
Weissella viridescens, Weissella viridescens CIP 102810 / ATCC 12706
brenda
Biarrotte-Sorin, S.; Maillard, A.P.; Delettre, J.; Sougakoff, W.; Arthur, M.; Mayer, C.
Crystal structures of Weissella viridescens FemX and its complex with UDP-MurNAc-pentapeptide: Insights into FemABX family substrates recognition
Structure
12
257-267
2004
Weissella viridescens (Q9EY50)
brenda
Chemama, M.; Fonvielle, M.; Arthur, M.; Valery, J.M.; Etheve-Quelquejeu, M.
Synthesis of stable aminoacyl-tRNA analogues containing triazole as a bioisoster of esters
Chemistry
15
1929-1938
2009
Weissella viridescens
brenda
Fonvielle, M.; Chemama, M.; Villet, R.; Lecerf, M.; Bouhss, A.; Valery, J.M.; Etheve-Quelquejeu, M.; Arthur, M.
Aminoacyl-tRNA recognition by the FemXWv transferase for bacterial cell wall synthesis
Nucleic Acids Res.
37
1589-1601
2009
Weissella viridescens
brenda
Mellal, D.; Fonvielle, M.; Santarem, M.; Chemama, M.; Schneider, Y.; Iannazzo, L.; Braud, E.; Arthur, M.; Etheve-Quelquejeu, M.
Synthesis and biological evaluation of non-isomerizable analogues of Ala-tRNAAla
Org. Biomol. Chem.
11
6161-6169
2013
Weissella viridescens
brenda
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