Information on EC 2.3.1.B6 - UDP-D-GlcNAc3NA 3-N-acetyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.B6
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
UDP-D-GlcNAc3NA 3-N-acetyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid + acetyl-CoA = UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronic acid + CoA
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
acatyl-CoA:UDP-2-acetamido-3-amino-2m3-dideoxy-D-glucuronic acid:acetyl-CoA 3-N-acetyltransferase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid + acetyl-CoA
UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronic acid + CoA
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid + acetyl-CoA
UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronic acid + CoA
show the reaction diagram
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?
additional information
?
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the enzyme is involved in the biosynthesis of a rare di-N-acetylated sugar in the lipopolysaccharide 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.107
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid
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30C, pH 7.0
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
48.3
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid
Pseudomonas aeruginosa PAO1
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30C, pH 7.0
25193
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, stable for at least 3 weeks
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22C, enzyme precipitates
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
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recombinant His6-tagged WbpD from Escherichia coli strain BL21(DE3)
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene wbpD, expression and subcloning of His6-tagged WbpD in Escherichia coli strain BL21(DE3)
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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construction of a gene wbpD knockout strain, functional complementation by expression of WbpB from Bordetella pertussis, overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis