Information on EC 2.3.1.B37 - phenylglycine N-acetyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Chryseobacterium

EC NUMBER
COMMENTARY hide
2.3.1.B37
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
phenylglycine N-acetyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + L-phenylglycine = (2S)-2-acetylamino-2-phenylacetic acid
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:L-phenylgylcine N-acetyltransferase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + (+/-)-1-phenylethylamine
CoA + ?
show the reaction diagram
acetyl-CoA + DL-2-chlorophenylglycine
CoA + ?
show the reaction diagram
acetyl-CoA + DL-2-phenylglycine
CoA + ?
show the reaction diagram
acetyl-CoA + DL-4-chlorophenylglycine
CoA + ?
show the reaction diagram
acetyl-CoA + L-2-chlorophenylglycine
CoA + N-acetyl-L-2-chlorophenylglycine
show the reaction diagram
52.9% activity compared to L-2-phenylglycine
-
-
?
acetyl-CoA + L-2-phenylglycine
CoA + (2S)-2-acetylamino-2-phenylacetic acid
show the reaction diagram
100% activity
-
-
?
acetyl-CoA + L-2-phenylglycine methyl ester
CoA + N-acetyl-L-2-phenylglycine methyl ester
show the reaction diagram
75.5% activity compared to L-2-phenylglycine
-
-
?
acetyl-CoA + L-4-hydroxyphenylglycine
CoA + N-acetyl-L-4-hydroxyphenylglycine
show the reaction diagram
16.7% activity compared to L-2-phenylglycine
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-chloromercuribenzoate
35.2% residual activity after 1 h at 1 mM
AgNO3-
22.4% residual activity after 1 h at 1 mM
Cu2+
79.5% residual activity after 1 h at 1 mM
diethyldicarbonate
49.3% residual activity after 1 h at 1 mM
Diethylpyrocarbonate
35.2% residual activity after 1 h at 1 mM
Hg2+
19.8% residual activity after 1 h at 1 mM
Mg2+
94.2% residual activity after 1 h at 1 mM
N-ethylmaleimide
34.5% residual activity after 1 h at 1 mM
o-phenanthroline
93.1% residual activity after 1 h at 1 mM
additional information
Mn2+, EDTA, tiron, alpha,alpha'-dipyridyl, phenylglyoxal, phenylmethylsulfonyl fluoride, iodoacetic acid and iodoacetamide do not markedly inhibit the enzyme
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.41
L-2-chlorophenylglycine
at pH 7.5 and 30°C
-
0.145
L-2-phenylglycine
at pH 7.5 and 30°C
-
0.77
L-2-phenylglycine methyl ester
at pH 7.5 and 30°C
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.31
L-2-chlorophenylglycine
Chryseobacterium sp.
W0SLW8
at pH 7.5 and 30°C
-
13.6
L-2-phenylglycine
Chryseobacterium sp.
W0SLW8
at pH 7.5 and 30°C
-
19.2
L-2-phenylglycine methyl ester
Chryseobacterium sp.
W0SLW8
at pH 7.5 and 30°C
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.55
cell extract, at pH 7.5 and 30°C
10.5
purified enzyme, at pH 7.5 and 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000
x * 18000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 50 mM sodium potassium phosphate buffer (pH 7.5) containing 0.3 M NaCl, more than 1 month, no loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, and Cosmogel His-accept column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells