Information on EC 2.3.1.B37 - phenylglycine N-acetyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Chryseobacterium

EC NUMBER
COMMENTARY hide
2.3.1.B37
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
phenylglycine N-acetyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + L-phenylglycine = (2S)-2-acetylamino-2-phenylacetic acid
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:L-phenylgylcine N-acetyltransferase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + (+/-)-1-phenylethylamine
CoA + ?
show the reaction diagram
acetyl-CoA + DL-2-chlorophenylglycine
CoA + ?
show the reaction diagram
acetyl-CoA + DL-2-phenylglycine
CoA + ?
show the reaction diagram
acetyl-CoA + DL-4-chlorophenylglycine
CoA + ?
show the reaction diagram
acetyl-CoA + L-2-chlorophenylglycine
CoA + N-acetyl-L-2-chlorophenylglycine
show the reaction diagram
52.9% activity compared to L-2-phenylglycine
-
-
?
acetyl-CoA + L-2-phenylglycine
CoA + (2S)-2-acetylamino-2-phenylacetic acid
show the reaction diagram
100% activity
-
-
?
acetyl-CoA + L-2-phenylglycine methyl ester
CoA + N-acetyl-L-2-phenylglycine methyl ester
show the reaction diagram
75.5% activity compared to L-2-phenylglycine
-
-
?
acetyl-CoA + L-4-hydroxyphenylglycine
CoA + N-acetyl-L-4-hydroxyphenylglycine
show the reaction diagram
16.7% activity compared to L-2-phenylglycine
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-chloromercuribenzoate
35.2% residual activity after 1 h at 1 mM
AgNO3-
22.4% residual activity after 1 h at 1 mM
Cu2+
79.5% residual activity after 1 h at 1 mM
diethyldicarbonate
49.3% residual activity after 1 h at 1 mM
Diethylpyrocarbonate
35.2% residual activity after 1 h at 1 mM
Hg2+
19.8% residual activity after 1 h at 1 mM
Mg2+
94.2% residual activity after 1 h at 1 mM
N-ethylmaleimide
34.5% residual activity after 1 h at 1 mM
o-phenanthroline
93.1% residual activity after 1 h at 1 mM
additional information
Mn2+, EDTA, tiron, alpha,alpha'-dipyridyl, phenylglyoxal, phenylmethylsulfonyl fluoride, iodoacetic acid and iodoacetamide do not markedly inhibit the enzyme
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.41
L-2-chlorophenylglycine
at pH 7.5 and 30C
-
0.145
L-2-phenylglycine
at pH 7.5 and 30C
-
0.77
L-2-phenylglycine methyl ester
at pH 7.5 and 30C
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.31
L-2-chlorophenylglycine
Chryseobacterium sp.
W0SLW8
at pH 7.5 and 30C
-
13.6
L-2-phenylglycine
Chryseobacterium sp.
W0SLW8
at pH 7.5 and 30C
-
19.2
L-2-phenylglycine methyl ester
Chryseobacterium sp.
W0SLW8
at pH 7.5 and 30C
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.55
cell extract, at pH 7.5 and 30C
10.5
purified enzyme, at pH 7.5 and 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, 50 mM sodium potassium phosphate buffer (pH 7.5) containing 0.3 M NaCl, more than 1 month, no loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, and Cosmogel His-accept column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells