Information on EC 2.3.1.B33 - chlorogenate caffeoyltransferase

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The expected taxonomic range for this enzyme is: Solanum lycopersicum

EC NUMBER
COMMENTARY hide
2.3.1.B33
-
RECOMMENDED NAME
GeneOntology No.
chlorogenate caffeoyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 chlorogenic acid = 1,5-dicaffeoylquinic acid + quinate
show the reaction diagram
-
-
-
-
2 chlorogenic acid = 3,5-dicaffeoylquinic acid + quinate
show the reaction diagram
-
-
-
-
2 chlorogenic acid = 4,5-dicaffeoylquinic acid + quinate
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
chlorogenate:chlorogenate caffeoyltransferase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bifunctional enzyme, displays hydroxycinnamoyl-Coenzyme A:quinate hydroxycinnamoyl transferase activity and chlorogenate:chlorogenate transferase activity
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 chlorogenic acid
1,5-dicaffeoylquinic acid + quinate
show the reaction diagram
reaction is irreversible
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
ir
2 chlorogenic acid
3,5-dicaffeoylquinic acid + quinate
show the reaction diagram
reaction is irreversible
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
ir
2-chlorogenic acid
4,5-dicaffeoylquinic acid + quinate
show the reaction diagram
reaction is irreversible
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
ir
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 chlorogenic acid
1,5-dicaffeoylquinic acid + quinate
show the reaction diagram
Q70G32
reaction is irreversible
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
ir
2 chlorogenic acid
3,5-dicaffeoylquinic acid + quinate
show the reaction diagram
Q70G32
reaction is irreversible
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
ir
2-chlorogenic acid
4,5-dicaffeoylquinic acid + quinate
show the reaction diagram
Q70G32
reaction is irreversible
products are 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid
-
ir
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
slight increase in activity
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
slight increase in activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
19
chlorogenic acid
pH 4.0, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22.3
pH 4.0, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
increase in CCT activity is observed during ripening, reaching a maximum in the peel of red fruit
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
enzyme catalyzes different reactions in different compartments
Manually annotated by BRENDA team
enzyme catalyzes different reactions in different compartments
Manually annotated by BRENDA team
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
homology modeling based on structure 4KEC of Sorghum bicolor hydroxycinnamoyl-coenzyme A transferase
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H276Y
58% of wild-type activity for production of dicaffeoylquinic acids, 92% of wild-type hydroxycinnamoyltranferase activity