Information on EC 2.3.1.B32 - [acyl-carrier-protein] S-methylmalonyltransferase

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The expected taxonomic range for this enzyme is: Saccharopolyspora erythraea

EC NUMBER
COMMENTARY hide
2.3.1.B32
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
[acyl-carrier-protein] S-methylmalonyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
methylmalonyl-CoA + an [acyl-carrier protein] = CoA + a methylmalonyl-[acyl-carrier protein]
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
methylmalonyl-CoA:[acyl-carrier protein] S-methylmalonyltransferase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Q03132, i.e. modules 3 and 4, of 6-deoxyerythronolide B synthase, EC 2.3.1.94
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ethylmalonyl-CoA + an [acyl-carrier protein]
CoA + a methylmalonyl-[acyl-carrier protein]
show the reaction diagram
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-
-
?
malonyl-CoA + an [acyl-carrier protein]
CoA + a methylmalonyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
?
methylmalonyl-CoA + an [acyl-carrier protein]
CoA + a methylmalonyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
?
additional information
?
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the cis-acyltransferase domain from DEBS module 3 transfers a methylmalonyl extender unit from the corresponding carboxyacyl-CoA precursor onto the phosphopantetheine arm of the acyl carrier protein within the same module. The presence of the acyl carrier protein has little effect on the specificity of the cis-acyltransferase domain of 6-deoxyerythronolide B synthase for carboxyacyl-CoA substrates, but has a marked influence on the corresponding specificity parameters for the trans-acyltransferases from the disorazole and kirromycin synthases. Comparative analysis of the substrate specificity
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
methylmalonyl-CoA
pH 7.6, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.033
methylmalonyl-CoA
pH 7.6, temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
ethylmalonyl-CoA
below 0.8 per sec and mM, pH 7.6, temperature not specified in the publication
0.8
malonyl-CoA
pH 7.6, temperature not specified in the publication
16.7
methylmalonyl-CoA
pH 7.6, temperature not specified in the publication