Information on EC 2.3.1.B16 - taxoid C-13 O-phenylpropanoyltransferase

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The expected taxonomic range for this enzyme is: Taxus cuspidata

EC NUMBER
COMMENTARY hide
2.3.1.B16
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
taxoid C-13 O-phenylpropanoyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
baccatin III + 3-amino-3-phenylpropanoyl-CoA = 3'-N-debenzoyltaxol + coenzyme A
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
baccatin III:(RS)-beta-phenylalanoyl-CoA beta-phenylalanoyltransferase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
baccatin III + 3-amino-3-phenylpropanoyl-CoA
3'-N-debenzoyltaxol + coenzyme A
show the reaction diagram
baccatin III + 3-phenylisoserinoyl-CoA
? + coenzyme A
show the reaction diagram
selective for 3-amino-3-phenylpropanoyl-CoA esters with a preference for beta-phenylalanoyl-CoA over 3-phenylisoserinoyl-CoA. Activity with 3-phenylisoserinoyl-CoA is 40% of the activity with beta-phenylalanoyl-CoA. alpha-Phenylalanoyl-CoA and N-benzoyl phenylisoserinoyl-CoA are not productive acyl donors
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
baccatin III + 3-amino-3-phenylpropanoyl-CoA
3'-N-debenzoyltaxol + coenzyme A
show the reaction diagram
Q8H2B5
the enzyme is involved in taxol biosynthesis
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0049
3-amino-3-phenylpropanoyl-CoA
pH and temperature not specified in the publication
0.0024
baccatin III
pH and temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8
recombinant enzyme
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
x * 50000, SDS-PAGE
50546
x * 50546, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 50000, SDS-PAGE; x * 50546, calculated from sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli BL21 (DE3)