Information on EC 2.3.1.73 - diacylglycerol-sterol O-acyltransferase

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The expected taxonomic range for this enzyme is: Spinacia oleracea

top print hide
EC NUMBER
COMMENTARY hide
2.3.1.73
-
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RECOMMENDED NAME
GeneOntology No.
diacylglycerol-sterol O-acyltransferase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 1,2-diacyl-sn-glycerol + sterol = a 1-acyl-sn-glycerol + sterol ester
show the reaction diagram
-
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Steroid biosynthesis
-
-
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SYSTEMATIC NAME
IUBMB Comments
1,2-diacyl-sn-glycerol:sterol O-acyltransferase
Cholesterol, sitosterol, campesterol and diacylglycerol can act as acceptors. Transfers a number of long-chain fatty acyl groups.
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top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
79586-23-5
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-diacyl-sn-glycerol + sterol
monoacylglycerol + sterol ester
show the reaction diagram
campesterol + 1,2-diacyl-sn-glycerol
monoacylglycerol + campesterol-3-O-acyl ester
show the reaction diagram
-
-
-
?
cholesterol + 1,2-diacyl-sn-glycerol
monoacylglycerol + cholesterol-3-O-acyl ester
show the reaction diagram
-
-
-
?
diacylglycerol + 1,2-diacyl-sn-glycerol
monoacylglycerol + triacyl-sn-glycerol
show the reaction diagram
-
-
-
?
dipalmitoylglycerol + sterol
monopalmitoylglycerol + steryl palmitate
show the reaction diagram
-
-
-
?
sitosterol + 1,2-diacyl-sn-glycerol
monoacylglycerol + sitosterol-3-O-acyl ester
show the reaction diagram
-
-
-
?
additional information
?
-
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Digitonin
-
effective inhibitor
ethanol
-
at 4% inhibits
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bovine serum albumin
-
stimulates
-
phosphatidylcholine
-
indispensable for activity
additional information
-
it is possible that Triton X-100 inhibits diacylglycerol degradation and that bovine serum albumin prevents this enzymic inhibition by binding the nonmicellar Triton X-100
-
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0002
-
leaf homogenate
0.0014
-
20000 * g pellet from differential centrifugation of the leaf homogenate
0.0022
-
88000 * g pellet from differential centrifugation of the leaf homogenate
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
MES-NaOH buffer
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
top print hide
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
differential centrifugation of the leaf homogenate shows the 20000 * g pellet to have the highest amount of enzyme of the fractions tested but the highest specific activity is in the 88000 * g pellet. The active fractions contain endoplasmic reticulum, ribosomes and other membrane vesicles
-
Manually annotated by BRENDA team
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15�C, in a desiccator, several weeks
-
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LINKS TO OTHER DATABASES (specific for EC-Number 2.3.1.73)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)