Information on EC 2.3.1.72 - indoleacetylglucose-inositol O-acyltransferase

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The expected taxonomic range for this enzyme is: Zea mays

EC NUMBER
COMMENTARY hide
2.3.1.72
-
RECOMMENDED NAME
GeneOntology No.
indoleacetylglucose-inositol O-acyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-O-(indol-3-yl)acetyl-beta-D-glucose + myo-inositol = D-glucose + O-(indol-3-yl)acetyl-myo-inositol
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
indole-3-acetyl-ester conjugate biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
1-O-(indol-3-yl)acetyl-beta-D-glucose:myo-inositol (indol-3-yl)acetyltransferase
The position of acylation is indeterminate because of the ease of acyl transfer between hydroxy groups.
CAS REGISTRY NUMBER
COMMENTARY hide
74082-57-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
indole-3-acetyl-beta-1-D-glucoside + myo-inositol
D-glucose + indole-3-acetyl-myo-inositol
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
indole-3-acetyl-beta-1-D-glucoside + myo-inositol
D-glucose + indole-3-acetyl-myo-inositol
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
74% inhibition at 5 mM
Dithionite
-
inhibition at 40 mM
dithiothreitol
-
98% inhibition at 5 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
1-O-indol-3-yl-acetyl-beta-D-glucose
-
-
4.1
myo-inositol
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
13.3
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9
-
-
additional information
-
pI: 6.1
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
immature
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
59000
-
gel filtration
100000
-
MW below 100000, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
enzyme exists as both a monomeric and homo- or heterodimeric form
monomer
-
1 * 654000, enzyme exists as both a monomeric and homo- or heterodimeric form, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
polypeptides of 56400 Da and 53500 Da have the same primary structure and the 3000 Da difference in molecular mass is probably caused by different glycosylation levels
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
lyophilization, stable to
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, crude liquid endosperm, several years, no loss of activity
-
4°C, more than 2 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
-