Information on EC 2.3.1.60 - gentamicin 3-N-acetyltransferase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
2.3.1.60
-
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RECOMMENDED NAME
GeneOntology No.
gentamicin 3-N-acetyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + gentamicin C = CoA + N3-acetylgentamicin C
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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-
-
-
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:gentamicin-C N3-acetyltransferase
Also acetylates sisomicin.
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CAS REGISTRY NUMBER
COMMENTARY hide
58500-58-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene aacC1; diverse clinical isolates, gene aacC1
UniProt
Manually annotated by BRENDA team
strain C600 containing R-factor JR88
-
-
Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
aminoglycoside acetyltransferase gene aac(3)-Id confers resistance to gentamicin and sisomicin
additional information
presence of genes encoding the following aminoglycoside-modifying en­zymes: aph (3')-V1, aph (3')-Ia, aac (3)-Ia, aac (3) IIa, aac (6')-Ih, aac (6')-Ib and ant (2')-Ia responsible for resis­tance in 4 different groups of clinical isolates, overview
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetoacetyl-CoA + gentamicin
CoA + N3'-acetoacetyl-gentamicin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + fortimicin A
CoA + N2'-acetylfortimicin A
show the reaction diagram
-
-
-
-
?
acetyl-CoA + gentamicin
CoA + N3'-acetyl-gentamicin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + gentamicin A
CoA + N3'-acetylgentamicin A
show the reaction diagram
-
-
-
-
?
acetyl-CoA + gentamicin B
CoA + N3'-acetylgentamicin B
show the reaction diagram
-
-
-
-
?
acetyl-CoA + gentamicin B1
CoA + N3'-acetylgentamicin B1
show the reaction diagram
-
-
-
-
?
acetyl-CoA + gentamicin C1
CoA + N3'-acetylgentamicin C1
show the reaction diagram
-
-
-
-
?
acetyl-CoA + gentamicin C1a
CoA + N3'-acetylgentamicin C1a
show the reaction diagram
acetyl-CoA + gentamicin C2
CoA + N3'-acetylgentamicin C2
show the reaction diagram
-
-
-
-
?
acetyl-CoA + gentamicin XK62-2
CoA + N3'-acetylgentamicin XK62-2
show the reaction diagram
-
-
-
-
?
acetyl-CoA + kanamycin B
CoA + N3'-acetylkanamycin B
show the reaction diagram
-
-
-
-
?
acetyl-CoA + N-ethylsisomicin
CoA + N3'-acetylethylsisomicin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + neamine
CoA + N3'-acetylneamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + nebramine
CoA + N2'-acetylnebramine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sisomicin
CoA + N3'-acetyl-sisomicin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
show the reaction diagram
acetyl-CoA + tobramycin
CoA + N3'-acetyltobramycin
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
O05286
determination of imipenem, meropenem, and gentamicin resistant clinical isolates
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-
-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-deoxystreptamine
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weak
amikacin
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i.e. BB-K 8
garamine
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-
Garamine A
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-
-
gentamicin
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-
gentamicin C1a
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substrate inhibition
Kanamine
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-
kanamycin A
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kanamycin C
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lividomycin
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-
Lividomycin B
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-
neomycin
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best inhibitor
paromomycin
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-
Paromomycin I
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-
ribostamycin
-
-
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
N-methylmaleimide and DTT have no effect on activity
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0039 - 0.0048
acetyl-CoA
-
-
0.0003 - 0.0005
gentamicin C1a
-
-
additional information
additional information
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kinetic pattern
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.57
N3'-acetylgentamicin C1a
Escherichia coli
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-
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
2-deoxystreptamine
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-
0.024
amikacin
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-
0.0036
garamine
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-
0.16
gentamicin
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-
0.208
Kanamine
-
-
0.093
kanamycin A
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-
0.026
kanamycin C
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-
0.002
lividomycin
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-
0.0004
neomycin
-
-
0.0083
paromomycin
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-
0.07
ribostamycin
-
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.7 - 8
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-
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 8.5
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-
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
analysis of enzyme expression in several clinical isolates, overview
Manually annotated by BRENDA team
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17000
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4 * 17000, SDS-PAGE
63000
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equilibrium sedimentation centrifugation
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
irreversible inactivation
637298
4.5
-
no activity, restorable by neutralization
637298
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
lyophilization, stable to
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
20°C, lyophilized, up to 1 month
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frozen, redissolved ammonium sulfate precipitate, at least 1 month
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refrigerated, lyophilized, up to 1 month
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene aac(3)-Id, DNA and amino acid sequence determination and analysis, detection and identification of class 1 and class 2 integrons and their antimicrobial resistance gene cassettes, overview
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gene aacC1, genotyping
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LINKS TO OTHER DATABASES (specific for EC-Number 2.3.1.60)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)