Information on EC 2.3.1.60 - gentamicin 3-N-acetyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.60
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RECOMMENDED NAME
GeneOntology No.
gentamicin 3-N-acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + gentamicin C = CoA + N3-acetylgentamicin C
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:gentamicin-C N3-acetyltransferase
Also acetylates sisomicin.
CAS REGISTRY NUMBER
COMMENTARY hide
58500-58-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene aacC1; diverse clinical isolates, gene aacC1
UniProt
Manually annotated by BRENDA team
strain C600 containing R-factor JR88
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
aminoglycoside acetyltransferase gene aac(3)-Id confers resistance to gentamicin and sisomicin
additional information
presence of genes encoding the following aminoglycoside-modifying en­zymes: aph (3')-V1, aph (3')-Ia, aac (3)-Ia, aac (3) IIa, aac (6')-Ih, aac (6')-Ib and ant (2')-Ia responsible for resis­tance in 4 different groups of clinical isolates, overview
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetoacetyl-CoA + gentamicin
CoA + N3'-acetoacetyl-gentamicin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + fortimicin A
CoA + N2'-acetylfortimicin A
show the reaction diagram
-
-
-
-
?
acetyl-CoA + gentamicin
CoA + N3'-acetyl-gentamicin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + gentamicin A
CoA + N3'-acetylgentamicin A
show the reaction diagram
-
-
-
-
?
acetyl-CoA + gentamicin B
CoA + N3'-acetylgentamicin B
show the reaction diagram
-
-
-
-
?
acetyl-CoA + gentamicin B1
CoA + N3'-acetylgentamicin B1
show the reaction diagram
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-
-
-
?
acetyl-CoA + gentamicin C1
CoA + N3'-acetylgentamicin C1
show the reaction diagram
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-
-
-
?
acetyl-CoA + gentamicin C1a
CoA + N3'-acetylgentamicin C1a
show the reaction diagram
acetyl-CoA + gentamicin C2
CoA + N3'-acetylgentamicin C2
show the reaction diagram
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-
-
-
?
acetyl-CoA + gentamicin XK62-2
CoA + N3'-acetylgentamicin XK62-2
show the reaction diagram
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-
-
-
?
acetyl-CoA + kanamycin B
CoA + N3'-acetylkanamycin B
show the reaction diagram
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-
-
-
?
acetyl-CoA + N-ethylsisomicin
CoA + N3'-acetylethylsisomicin
show the reaction diagram
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-
-
-
?
acetyl-CoA + neamine
CoA + N3'-acetylneamine
show the reaction diagram
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-
-
-
?
acetyl-CoA + nebramine
CoA + N2'-acetylnebramine
show the reaction diagram
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-
-
-
?
acetyl-CoA + sisomicin
CoA + N3'-acetyl-sisomicin
show the reaction diagram
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-
-
-
?
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
show the reaction diagram
acetyl-CoA + tobramycin
CoA + N3'-acetyltobramycin
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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O05286
determination of imipenem, meropenem, and gentamicin resistant clinical isolates
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-deoxystreptamine
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weak
amikacin
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i.e. BB-K 8
garamine
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Garamine A
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gentamicin
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gentamicin C1a
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substrate inhibition
Kanamine
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kanamycin A
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kanamycin C
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lividomycin
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Lividomycin B
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neomycin
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best inhibitor
paromomycin
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Paromomycin I
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ribostamycin
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-
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
N-methylmaleimide and DTT have no effect on activity
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0039 - 0.0048
acetyl-CoA
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0.0003 - 0.0005
gentamicin C1a
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-
additional information
additional information
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kinetic pattern
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.57
N3'-acetylgentamicin C1a
Escherichia coli
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
2-deoxystreptamine
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0.024
amikacin
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0.0036
garamine
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0.16
gentamicin
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0.208
Kanamine
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0.093
kanamycin A
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0.026
kanamycin C
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0.002
lividomycin
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0.0004
neomycin
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0.0083
paromomycin
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0.07
ribostamycin
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.7 - 8
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 8.5
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
analysis of enzyme expression in several clinical isolates, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17000
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4 * 17000, SDS-PAGE
63000
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equilibrium sedimentation centrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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irreversible inactivation
637298
4.5
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no activity, restorable by neutralization
637298
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
lyophilization, stable to
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
20°C, lyophilized, up to 1 month
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frozen, redissolved ammonium sulfate precipitate, at least 1 month
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refrigerated, lyophilized, up to 1 month
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene aac(3)-Id, DNA and amino acid sequence determination and analysis, detection and identification of class 1 and class 2 integrons and their antimicrobial resistance gene cassettes, overview
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gene aacC1, genotyping