Information on EC 2.3.1.59 - gentamicin 2'-N-acetyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.59
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RECOMMENDED NAME
GeneOntology No.
gentamicin 2'-N-acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + gentamicin C1a = CoA + N2'-acetylgentamicin C1a
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:gentamicin-C1a N2'-acetyltransferase
The antibiotics gentamicin A, sisomicin, tobramycin, paromomycin, neomycin B, kanamycin B and kanamycin C can also act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
50864-40-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Morganella morganii
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Manually annotated by BRENDA team
no activity in Proteus myxofaciens
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Manually annotated by BRENDA team
no activity in Proteus vulgaris
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-
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Manually annotated by BRENDA team
no activity in Providencia heimbachae
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Manually annotated by BRENDA team
no activity in Providencia rustigianii
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain 164, clinical isolate
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Manually annotated by BRENDA team
strain 164, clinical isolate
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + butirosin
CoA + ?
show the reaction diagram
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acetylated at 30% the rate of gentamicin C1
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?
acetyl-CoA + dideoxykanamycin
CoA + N2'-diacetyldideoxykanamycin
show the reaction diagram
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acetylated at 92% the rate of gentamicin C1
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?
acetyl-CoA + gentamicin B
CoA + N2'-acetylgentamycin
show the reaction diagram
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7% of the activity with gentamicin C1
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?
acetyl-CoA + gentamicin C1
CoA + N2'-acetylgentamicin C1
show the reaction diagram
acetyl-CoA + gentamicin C1a
CoA + N2'-acetylgentamicin C1a
show the reaction diagram
acetyl-CoA + kanamycin B
CoA + N2-acetylkanamycin B
show the reaction diagram
acetyl-CoA + kanamycin C
CoA + N2'-acetylkanamycin C
show the reaction diagram
acetyl-CoA + neomycin B
CoA + N2'-acetylneomycin
show the reaction diagram
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acetylated at 10% the rate of gentamicin C1
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?
acetyl-CoA + paromomycin
CoA + N2'-acetylparomomycin
show the reaction diagram
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?
acetyl-CoA + ribostamycin
CoA + N2'-acetylribostamycin
show the reaction diagram
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acetylated at 15% the rate of gentamicin C1
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?
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
show the reaction diagram
acetyl-CoA + tobramycin
CoA + N2'-acetyltobramycin
show the reaction diagram
acetyl-CoA of soluble fragments of peptidoglycan + tobramycin
?
show the reaction diagram
N-acetyl-D-glucosamine + tobramycin
D-glucosamine + N2'-acetyltobramycin
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
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kanamycin C
6.6
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gentamicin C
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7 - 6.5
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about half-maximal activity at pH 5.7 and 6.5, kanamycin C
6.4 - 7.6
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about half-maximal activity at pH 6.4 and 7.6, gentamicin C
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity in crude enzyme preparation is stable to repeated freezing and thawing
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis