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Information on EC 2.3.1.59 - gentamicin 2'-N-acetyltransferase Word Map on EC 2.3.1.59
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The enzyme appears in viruses and cellular organisms
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gentamicin 2'-N-acetyltransferase
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acetyl-CoA + gentamicin C1a = CoA + N2'-acetylgentamicin C1a
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Acyl group transfer
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acetyl-CoA:gentamicin-C1a N2'-acetyltransferase
The antibiotics gentamicin A, sisomicin, tobramycin, paromomycin, neomycin B, kanamycin B and kanamycin C can also act as acceptors.
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acetyltransferase, gentamicin 2'-
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gentamicin acetyltransferase II
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no activity in Morganella morganii
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brenda
no activity in Proteus myxofaciens
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brenda
no activity in Proteus vulgaris
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brenda
no activity in Providencia heimbachae
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brenda
no activity in Providencia rustigianii
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brenda
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brenda
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brenda
strain 164, clinical isolate
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brenda
strain 164, clinical isolate
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brenda
PR50
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brenda
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brenda
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brenda
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brenda
PR50
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brenda
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acetyl-CoA + butirosin
CoA + ?
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acetylated at 30% the rate of gentamicin C1
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?
acetyl-CoA + dideoxykanamycin
CoA + N2'-diacetyldideoxykanamycin
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acetylated at 92% the rate of gentamicin C1
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?
acetyl-CoA + gentamicin B
CoA + N2'-acetylgentamycin
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7% of the activity with gentamicin C1
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?
acetyl-CoA + gentamicin C1
CoA + N2'-acetylgentamicin C1
acetyl-CoA + gentamicin C1a
CoA + N2'-acetylgentamicin C1a
acetyl-CoA + kanamycin B
CoA + N2-acetylkanamycin B
acetyl-CoA + kanamycin C
CoA + N2'-acetylkanamycin C
acetyl-CoA + neomycin B
CoA + N2'-acetylneomycin
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acetylated at 10% the rate of gentamicin C1
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?
acetyl-CoA + paromomycin
CoA + N2'-acetylparomomycin
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?
acetyl-CoA + ribostamycin
CoA + N2'-acetylribostamycin
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acetylated at 15% the rate of gentamicin C1
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?
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
acetyl-CoA + tobramycin
CoA + N2'-acetyltobramycin
acetyl-CoA of soluble fragments of peptidoglycan + tobramycin
?
N-acetyl-D-glucosamine + tobramycin
D-glucosamine + N2'-acetyltobramycin
additional information
?
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acetyl-CoA + gentamicin C1
CoA + N2'-acetylgentamicin C1
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best substrate
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?
acetyl-CoA + gentamicin C1
CoA + N2'-acetylgentamicin C1
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best substrate
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?
acetyl-CoA + gentamicin C1
CoA + N2'-acetylgentamicin C1
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?
acetyl-CoA + gentamicin C1a
CoA + N2'-acetylgentamicin C1a
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acetylated at 86% the rate of gentamicin C1
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?
acetyl-CoA + gentamicin C1a
CoA + N2'-acetylgentamicin C1a
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acetylated at 86% the rate of gentamicin C1
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?
acetyl-CoA + gentamicin C1a
CoA + N2'-acetylgentamicin C1a
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?
acetyl-CoA + gentamicin C1a
CoA + N2'-acetylgentamicin C1a
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acetylates 2'-amino group of hexose moiety
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?
acetyl-CoA + kanamycin B
CoA + N2-acetylkanamycin B
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acetylated at 14% the rate of gentamicin C1
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?
acetyl-CoA + kanamycin B
CoA + N2-acetylkanamycin B
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?
acetyl-CoA + kanamycin C
CoA + N2'-acetylkanamycin C
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acetylated at 40% the rate of gentamicin C1
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?
acetyl-CoA + kanamycin C
CoA + N2'-acetylkanamycin C
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?
acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
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acetylated at 71% the rate of gentamicin C1
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acetyl-CoA + sisomicin
CoA + N3'-acetylsisomicin
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acetylated at 71% the rate of gentamicin C1
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acetyl-CoA + tobramycin
CoA + N2'-acetyltobramycin
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acetylated at 58% the rate of gentamicin C1
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?
acetyl-CoA + tobramycin
CoA + N2'-acetyltobramycin
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acetylated at 58% the rate of gentamicin C1
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?
acetyl-CoA + tobramycin
CoA + N2'-acetyltobramycin
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?
acetyl-CoA of soluble fragments of peptidoglycan + tobramycin
?
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acetylated at 58% the rate of gentamicin C1
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?
acetyl-CoA of soluble fragments of peptidoglycan + tobramycin
?
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?
N-acetyl-D-glucosamine + tobramycin
D-glucosamine + N2'-acetyltobramycin
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acetylated at 58% the rate of gentamicin C1
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?
N-acetyl-D-glucosamine + tobramycin
D-glucosamine + N2'-acetyltobramycin
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?
additional information
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no substrates are kanamycin A and 1-hydroxyaminobutyric acid kanamycin A-derivative BB-K 8
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additional information
?
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no substrates are kanamycin A and 1-hydroxyaminobutyric acid kanamycin A-derivative BB-K 8
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additional information
?
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the enzyme may contribute to the O-acetylation of peptidoglycan
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?
additional information
?
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enzyme may have a physiological role in secondary metabolism, its activity on aminoglycosides is simply fortouitous
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?
additional information
?
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the enzyme contributes to the O-acetylation of peptidoglycan. Mutants that either underexpress or overexpress the aac(2')-Ia gene are characterized phenotypically to possess either lower or higher levels of peptidoglycan O-acetylation, respectively, compared to the wild-type. Providencia stuartii PR100, with 42-44% peptidoglycan O-acetylation compared to 54% of the wild-type, appears as irregular rods. Strains PR50.LM3 and PR51, with increased levels of peptidoglycan O-acetylation, 63% and 65% respectively, appear as coccobacilli or chain formers
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?
additional information
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the enzyme may contribute to the O-acetylation of peptidoglycan
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?
additional information
?
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additional information
?
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modification of antibiotics
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?
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additional information
?
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additional information
?
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the enzyme may contribute to the O-acetylation of peptidoglycan
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?
additional information
?
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enzyme may have a physiological role in secondary metabolism, its activity on aminoglycosides is simply fortouitous
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?
additional information
?
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the enzyme contributes to the O-acetylation of peptidoglycan. Mutants that either underexpress or overexpress the aac(2')-Ia gene are characterized phenotypically to possess either lower or higher levels of peptidoglycan O-acetylation, respectively, compared to the wild-type. Providencia stuartii PR100, with 42-44% peptidoglycan O-acetylation compared to 54% of the wild-type, appears as irregular rods. Strains PR50.LM3 and PR51, with increased levels of peptidoglycan O-acetylation, 63% and 65% respectively, appear as coccobacilli or chain formers
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?
additional information
?
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the enzyme may contribute to the O-acetylation of peptidoglycan
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?
additional information
?
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modification of antibiotics
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?
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5.7 - 6.5
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about half-maximal activity at pH 5.7 and 6.5, kanamycin C
6.4 - 7.6
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about half-maximal activity at pH 6.4 and 7.6, gentamicin C
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brenda
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activity in crude enzyme preparation is stable to repeated freezing and thawing
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analysis
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rapid analysis of antibiotic concentration in serum
analysis
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rapid analysis of antibiotic concentration in serum
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AAC2_PROST
178
20087
Swiss-Prot
A0A1V2MP07_9ACTN
221
23648
TrEMBL
A0A1V2MMI1_9ACTN
183
19472
TrEMBL
A0A1V2MIF2_9ACTN
174
18537
TrEMBL
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Benveniste, R.; Davies, J.
Aminoglycoside antibiotic-inactivating enzymes in actinomycetes similar to those present in clinical isolates of antibiotic-resistant bacteria
Proc. Natl. Acad. Sci. USA
70
2276-2280
1973
Streptomyces kanamyceticus, Streptomyces spectabilis
brenda
Chevereau, M.; Daniels, P.J.L.; Davies, J.; LeGoffic, F.
Aminoglycoside resistance in bacteria medicated by gentamicin acetyltransferase II, an enzyme modifying the 2-amino group of aminoglycoside antibiotics
Biochemistry
13
598-603
1974
Providencia sp. 164, Providencia sp.
brenda
Payie, K.G.; Clarke, A.J.
Characterization of gentamicin 2'-N-acetyltransferase from Providencia stuartii: its use of peptidoglycan metabolites for acetylation of both aminoglycosides and peptidoglycan
J. Bacteriol.
179
4106-4114
1997
Providencia stuartii
brenda
Clarke, A.J.; Francis, D.; Keenleyside, W.J.
The prevalence of gentamicin 2'-N-acetyltransferase in the Proteeae and its role in the O-acetylation of peptidoglycan
FEMS Microbiol. Lett.
145
201-207
1996
no activity in Morganella morganii, no activity in Proteus myxofaciens, no activity in Proteus vulgaris, no activity in Providencia heimbachae, no activity in Providencia rustigianii, Proteus penneri, Providencia rettgeri, Providencia stuartii, Providencia stuartii PR50
brenda
Payie, K.G.; Strating, H.; Clarke, A.J.
The role of O-acetylation in the metabolism of peptidoglycan in Providencia stuartii
Microb. Drug Resist.
2
135-140
1996
Providencia stuartii
brenda
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