Information on EC 2.3.1.56 - aromatic-hydroxylamine O-acetyltransferase

Word Map on EC 2.3.1.56
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.3.1.56
-
RECOMMENDED NAME
GeneOntology No.
aromatic-hydroxylamine O-acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-hydroxy-4-acetylaminobiphenyl + N-hydroxy-4-aminobiphenyl = N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminobiphenyl
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
N-hydroxy-4-acetylaminobiphenyl:N-hydroxy-4-aminobiphenyl O-acetyltransferase
Transfers the N-acetyl group of some aromatic acethydroxamates to the O-position of some aromatic hydroxylamines.
CAS REGISTRY NUMBER
COMMENTARY hide
52660-15-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
mongrel dogs
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity in Cavia porcellus
-
-
-
Manually annotated by BRENDA team
no activity in Mus musculus
-
-
-
Manually annotated by BRENDA team
Sprague-Dawley CD strain
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7-acetyl-N-hydroxy-2-acetylaminofluorene + 4-aminoazobenzene
7-acetyl-N-hydroxy-2-aminofluorene + N-acetoxy-4-aminoazobenzene
show the reaction diagram
-
-
-
-
?
7-bromo-N-hydroxy-2-acetylaminofluorene + 4-aminoazobenzene
7-bromo-N-hydroxy-2-aminofluorene + N-acetoxy-4-aminoazobenzene
show the reaction diagram
-
-
-
-
?
7-ethoxy-N-hydroxy-2-acetylaminofluorene + 4-aminoazobenzene
7-ethoxy-N-hydroxy-2-aminofluorene + N-acetoxy-4-aminoazobenzene
show the reaction diagram
-
-
-
-
?
7-ethyl-N-hydroxy-2-acetylaminofluorene + 4-aminoazobenzene
7-ethyl-N-hydroxy-2-aminofluorene + N-acetoxy-4-aminoazobenzene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminoveratrole
CoA + N-acetyl-4-aminoveratrole
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-anisidine
CoA + N-acetyl-4-anisidine
show the reaction diagram
acetyl-CoA + 4-iodoaniline
CoA + N-acetyl-4-iodoaniline
show the reaction diagram
N-hydroxy-2-acetylaminofluorene + 4-aminoazobenzene
N-hydroxy-2-aminofluorene + N-acetoxy-4-aminoazobenzene
show the reaction diagram
-
-
-
-
?
N-hydroxy-2-acetylaminofluorene + N-hydroxy-4-aminobiphenyl
N-hydroxy-2-aminofluorene + N-acetoxy-4-aminobiphenyl
show the reaction diagram
N-hydroxy-2-propylaminofluorene + ?
?
show the reaction diagram
-
i.e. N-hydroxy-N2-fluorenylpropionamide
-
-
?
N-hydroxy-4-acetylaminobiphenyl + 4-aminoazobenzene
N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminoazobenzene
show the reaction diagram
N-hydroxy-4-acetylaminobiphenyl + N-hydroxy-4-aminobiphenyl
N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminobiphenyl
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-hydroxy-4-acetylaminobiphenyl + N-hydroxy-4-aminobiphenyl
N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminobiphenyl
show the reaction diagram
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-(fluoren-2-yl)-2-propen-1-one
-
irreversible and selective inactivation
7-Substituted N-hydroxy-2-acetamidofluorenes
-
residues: H, halo, CN, acetoxy, methoxy, ethoxy, butoxy, inhibition kinetics
iodoacetamide
-
-
N-Acetamido-N-hydroxy-7-methoxyfluorene
-
inhibition kinetics
N-ethylmaleimide
-
-
N-Hydroxy-2-acetylaminofluorene
N-Hydroxy-4-acetylaminobiphenyl
puromycin
-
weak
trans-N-Hydroxy-4-acetylaminostilbene
additional information
-
no inhibition by paraoxon
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
reduced pyridine nucleotides
-
mammary gland
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00064 - 0.138
4-aminoazobenzene
0.0063
N-Hydroxy-2-acetylaminofluorene
-
-
0.005 - 0.356
N-Hydroxy-4-acetylaminobiphenyl
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0075
-
7-ethyl-N-hydroxy-2-acetylaminofluorene + 4-aminoazobenzene
0.025
recombinant enzyme, N-acetylation of 4-aminoazobenzene
0.036
recombinant enzyme, N-acetylation of 4-aminoazobenzene
0.084
recombinant enzyme, N-acetylation of 4-aminoazobenzene
0.097
recombinant enzyme, N-acetylation of 4-aminoazobenzene
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
nucleic acid adduct formation assay
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6 - 9.6
-
about half-maximal activity at pH 5.6 and pH 9.6, nucleic acid adduct formation assay
6.8 - 8.2
-
about 80% of maximal activity at pH 6.8 and pH 8.2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
nucleic acid adduct formation assay
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 72
-
about half-maximal activity at 25C and 72C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
of liver and urinary bladder
Manually annotated by BRENDA team
-
alveolar cells
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29000
x * 29000, isoform NAT2, SDS-PAGE
32000
-
gel filtration
38500
-
x * 38500, SDS-PAGE
60000
-
3 * 60000, SDS-PAGE
180000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 32000, SDS-PAGE
trimer
-
3 * 60000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
mannose-rich glycoprotein
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting and hanging drop methods, 20C, 10 mg/ml protein solution: 10 mM Tris-HCl, pH 7.9, 0.1 M NaCl, 3 mM dithiothreitol, reservoir solution: 0.8 M Na,K tartrate, 0.1 M MES, pH 7.1, X-ray diffraction
-
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
argon-saturated diphosphate buffer stabilizes
-
freezing and thawing inactivates
-
lyophilization inactivates
-
purification inactivates
-
unstable in low salt solutions
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable in the presence of oxygen due to labile SH-groups, dithiothreitol cannot fully stabilize
-
487243
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, up to 3 months in saturating ammonium sulfate sealed under argon
-
0-4C, about 30% loss of activity after 6 h
-
0-4C, inactivation after 18 h
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially
recombinant His-tagged protein from E. coli, removal of His-tag
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression as His-tagged protein in Escherichia coli
-
expression of isoform NAT1 in Escherichia coli; expression of isoform NAT2 in Escherichia coli
expression of isoforms 1 and 2 in chinese hamster lung fibroblast derived cell line V79MZ and in TA1538-derived Salmonella typhimurium strains
-
expression of isoforms NAT1 in Escherichia coli; expression of isoforms NAT2 in Escherichia coli
expression of wild-type and mutants in Escherichia coli strains DH5alpha and JM105
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G286E
-
small reduction in activity
I114T
-
strong reduction of the acetylation activity
K268R
-
slightly increased activity
R197Q
-
moderate reduction of acetylation activity
R64Q
-
moderate reduction of acetylation activity
additional information
-
nucleic acid substitutions C282T and C481T have no effect on activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine