Information on EC 2.3.1.40 - acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.3.1.40
-
RECOMMENDED NAME
GeneOntology No.
acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an acyl-[acyl-carrier protein] + O-(2-acyl-sn-glycero-3-phospho)ethanolamine = an [acyl-carrier protein] + O-(1,2-diacyl-sn-glycero-3-phospho)ethanolamine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycerophospholipid metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
acyl-[acyl-carrier protein]:O-(2-acyl-sn-glycero-3-phospho)ethanolamine O-acyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
37257-18-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
squash
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyl-(acyl-carrier protein) + O-(2-acyl-sn-glycero-3-phospho) ethanolamine
(acyl-carrier protein) + O-(1,2-diacyl-sn-glycero-3-phospho) ethanolamine
show the reaction diagram
-
specific for C1-position of glycerol-phosphate backbone, the acyl-component of the acyl-(acyl-carrier-protein) may be 3-hydroxydecanoate, 3-hydroxylaurate, 3-hydroxymyristate and 3-hydroxyoctanoate (tentatively identified)
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
activation, 0.2 M
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no stimulation by coenzyme A, ATP, GTP, UTP, glucosamine 1-phosphate, UDP-N-acetylglucosamine, Triton X-100, lipid A, alkaline treated lipid A or alkaline treated lipid A annealed with Escherichia coli phospholipids
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
pI at 4°C: 5.5 (isozyme AT3), 5.6 (isozyme AT2), 6.6 (isozyme AT1)
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10
-
about half-maximal activity at pH 5.0, about 60% of maximal activity at pH 10.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
greening
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
isozyme AT1, gel filtration
40000
-
isozyme AT2 and isozyme AT3, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 30000, isozyme AT1, 1 * 40000, isozymes AT2 and AT3, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
inactivation after 2-5 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
acyl-carrier-protein affinity chromatography
-
partial, solubilization by sonic disruption
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H36A
-
mutant lacks 2-acylglycerophosphoethanolamine acyltransferase activity, whereas it retains acyl-acyl carrier protein synthase activity
Show AA Sequence (188 entries)
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