Information on EC 2.3.1.36 - D-amino-acid N-acetyltransferase

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The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae

EC NUMBER
COMMENTARY hide
2.3.1.36
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RECOMMENDED NAME
GeneOntology No.
D-amino-acid N-acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + a D-amino acid = CoA + an N-acetyl-D-amino acid
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Phenylalanine metabolism
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:D-amino-acid N-acetyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
37257-15-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + a D-amino acid
CoA + an N-acetyl-D-amino acid
show the reaction diagram
acetyl-CoA + D-alanine
CoA + N-acetyl-D-alanine
show the reaction diagram
acetyl-CoA + D-alpha-aminobutyrate
CoA + N-acetyl-D-alpha-aminobutyrate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + D-arginine
CoA + N-acetyl-D-arginine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + D-asparaginate
CoA + N-acetyl-D-asparaginate
show the reaction diagram
-
-
-
-
ir
acetyl-CoA + D-asparagine
CoA + N-acetyl-D-asparagine
show the reaction diagram
acetyl-CoA + D-cysteine
CoA + N-acetyl-D-cysteine
show the reaction diagram
-
-
-
-
ir
acetyl-CoA + D-cystine
CoA + N-acetyl-D-cystine
show the reaction diagram
-
-
-
-
ir
acetyl-CoA + D-glutamine
CoA + N-acetyl-D-glutamine
show the reaction diagram
acetyl-CoA + D-histidine
CoA + N-acetyl-D-histidine
show the reaction diagram
-
-
-
-
ir
acetyl-CoA + D-isoleucine
CoA + N-acetyl-D-isoleucine
show the reaction diagram
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-
-
-
ir
acetyl-CoA + D-leucine
CoA + N-acetyl-D-leucine
show the reaction diagram
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-
-
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ir
acetyl-CoA + D-lysine
CoA + N-acetyl-D-lysine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + D-methionine
CoA + N-acetyl-D-methionine
show the reaction diagram
acetyl-CoA + D-norleucine
CoA + N-acetyl-D-norleucine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + D-norvaline
CoA + N-acetyl-D-norvaline
show the reaction diagram
acetyl-CoA + D-phenylalanine
CoA + N-acetyl-D-phenylalanine
show the reaction diagram
acetyl-CoA + D-proline
CoA + N-acetyl-D-proline
show the reaction diagram
-
-
-
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ir
acetyl-CoA + D-serine
CoA + N-acetyl-D-serine
show the reaction diagram
acetyl-CoA + D-threonine
CoA + N-acetyl-D-threonine
show the reaction diagram
acetyl-CoA + D-tryptophan
CoA + N-acetyl-D-tryptophan
show the reaction diagram
acetyl-CoA + D-tyrosine
CoA + N-acetyl-D-tyrosine
show the reaction diagram
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-
-
-
ir
acetyl-CoA + D-valine
CoA + N-acetyl-D-valine
show the reaction diagram
acetyl-CoA + norleucine
CoA + N-acetyl-D-norleucine
show the reaction diagram
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-
-
-
ir
propionyl-CoA + D-alanine
CoA + N-propionyl-D-alanine
show the reaction diagram
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-
-
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ir
propionyl-CoA + D-allo-isoleucine
CoA + N-propionyl-D-allo-isoleucine
show the reaction diagram
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-
-
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ir
propionyl-CoA + D-glutamine
CoA + N-propionyl-D-glutamine
show the reaction diagram
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-
-
-
ir
propionyl-CoA + D-leucine
CoA + N-propionyl-D-leucine
show the reaction diagram
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-
-
-
ir
propionyl-CoA + D-serine
CoA + N-propionyl-D-serine
show the reaction diagram
-
-
-
-
ir
propionyl-CoA + D-tryptophan
CoA + N2-propionyl-D-tryptophan
show the reaction diagram
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-
-
ir
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + a D-amino acid
CoA + an N-acetyl-D-amino acid
show the reaction diagram
acetyl-CoA + D-alanine
CoA + N-acetyl-D-alanine
show the reaction diagram
-
-
-
-
ir
acetyl-CoA + D-asparaginate
CoA + N-acetyl-D-asparaginate
show the reaction diagram
-
-
-
-
ir
acetyl-CoA + D-asparagine
CoA + N-acetyl-D-asparagine
show the reaction diagram
-
-
-
-
ir
acetyl-CoA + D-cysteine
CoA + N-acetyl-D-cysteine
show the reaction diagram
-
-
-
-
ir
acetyl-CoA + D-cystine
CoA + N-acetyl-D-cystine
show the reaction diagram
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-
-
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ir
acetyl-CoA + D-glutamine
CoA + N-acetyl-D-glutamine
show the reaction diagram
-
-
-
-
ir
acetyl-CoA + D-histidine
CoA + N-acetyl-D-histidine
show the reaction diagram
-
-
-
-
ir
acetyl-CoA + D-isoleucine
CoA + N-acetyl-D-isoleucine
show the reaction diagram
-
-
-
-
ir
acetyl-CoA + D-leucine
CoA + N-acetyl-D-leucine
show the reaction diagram
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-
-
-
ir
acetyl-CoA + D-methionine
CoA + N-acetyl-D-methionine
show the reaction diagram
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-
-
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ir
acetyl-CoA + D-norvaline
CoA + N-acetyl-D-norvaline
show the reaction diagram
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-
-
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ir
acetyl-CoA + D-phenylalanine
CoA + N-acetyl-D-phenylalanine
show the reaction diagram
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-
-
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ir
acetyl-CoA + D-proline
CoA + N-acetyl-D-proline
show the reaction diagram
-
-
-
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ir
acetyl-CoA + D-serine
CoA + N-acetyl-D-serine
show the reaction diagram
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-
-
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ir
acetyl-CoA + D-threonine
CoA + N-acetyl-D-threonine
show the reaction diagram
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-
-
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ir
acetyl-CoA + D-tryptophan
CoA + N-acetyl-D-tryptophan
show the reaction diagram
-
-
-
ir
acetyl-CoA + D-tyrosine
CoA + N-acetyl-D-tyrosine
show the reaction diagram
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-
-
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ir
acetyl-CoA + D-valine
CoA + N-acetyl-D-valine
show the reaction diagram
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-
-
-
ir
acetyl-CoA + norleucine
CoA + N-acetyl-D-norleucine
show the reaction diagram
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-
-
-
ir
propionyl-CoA + D-alanine
CoA + N-propionyl-D-alanine
show the reaction diagram
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-
-
-
ir
propionyl-CoA + D-allo-isoleucine
CoA + N-propionyl-D-allo-isoleucine
show the reaction diagram
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-
-
-
ir
propionyl-CoA + D-glutamine
CoA + N-propionyl-D-glutamine
show the reaction diagram
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-
-
-
ir
propionyl-CoA + D-leucine
CoA + N-propionyl-D-leucine
show the reaction diagram
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-
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ir
propionyl-CoA + D-serine
CoA + N-propionyl-D-serine
show the reaction diagram
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-
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ir
propionyl-CoA + D-tryptophan
CoA + N2-propionyl-D-tryptophan
show the reaction diagram
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-
-
ir
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
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strong inhibitor at 1 mM
CoA
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competitive against acetyl-CoA, noncompetitive against D-tryptophan
o-Iodosobenzoate
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strong inhibitor at 1 mM
p-chloromercuribenzoate
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strong inhibitor at 1 mM
ZnCl2
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strong inhibitor at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.227 - 0.7
acetyl-CoA
0.054
D-alanine
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30°C, recombinant enzyme
0.034
D-serine
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30°C, recombinant enzyme
4.5 - 5.5
D-tryptophan
0.263
propionyl-CoA
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additional information
additional information
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kinetics
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.97
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D-tryptophan
additional information
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substrate specificity
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3 - 7.5
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in phosphate buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 21000, recombinant intact Hpa3p, SDS-PAGE, x * 18000, recombinant N-terminally truncated enzyme, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, stable for several months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme from dry yeast, to homogeneity in several steps, recombinant enzyme from Escherichia coli in a procedure similar to the purification of the native enzyme to 95% purity
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to homogeneity by ammonium sulfate fractionation, DEAE ion exchange chromatography, chromatography on a butyl resin, gel filtration, and D-glutamate affinity chromatography, overview
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene HPA3, functional overexpression in Escherichia coli
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when the gene is overexpressed with a vector plasmid p426Gal1 in the wild type Saccharomyces cerevisiae as a host, the recombinant yeast, which shows more than 100 times higher D-amino acid-N-acetyltransferase activity than the wild type, is much more tolerant to D-amino acids than the wild type
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