Information on EC 2.3.1.33 - histidine N-acetyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.33
-
RECOMMENDED NAME
GeneOntology No.
histidine N-acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + L-histidine = CoA + N-acetyl-L-histidine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:L-histidine N-acetyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9027-59-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
killifish
-
-
Manually annotated by BRENDA team
gene Nat16
UniProt
Manually annotated by BRENDA team
gene hisat, two HISAT splice variant-1 and -2
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + 1-methyl-L-histidine
CoA + 1-methyl-N-acetyl-L-histidine
show the reaction diagram
-
23% activity compared to that with L-histidine
-
?
acetyl-CoA + 3-methyl-L-histidine
CoA + 3-methyl-N-acetyl-L-histidine
show the reaction diagram
-
71% activity compared to that with L-histidine
-
?
acetyl-CoA + L-histidine
CoA + N-acetyl-L-histidine
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + L-histidine
CoA + N-acetyl-L-histidine
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetyl-CoA
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
stimulates
additional information
-
no divalent metal ions required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
slightly inhibitory
coenzyme A
-
-
Cu2+
-
complete inhibition
dithiothreitol
-
-
Hg2+
-
strong inhibition
hydroxylamine
-
slightly inhibitory
N-ethylmaleimide
-
-
N-ethylmorpholine-HCl buffer
-
slightly inhibitory
-
Ni2+
-
strong inhibition
o-phenanthroline
-
slightly inhibitory
p-chloromercuribenzoate
-
strong inhibition
Tris-HCl buffer
-
slightly inhibitory
additional information
-
no inhibition by EDTA, Cd2+, Mg2+, Mn2+, Sr2+, Ca2+, Pb2+
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetyl-CoA
-
15fold stimulation
Acetylcholine
-
2.5fold stimulation
ATP
-
2.6fold stimulation
glycine
-
1.9fold stimulation
Sodium acetate
-
3.1fold stimulation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.027
acetylcoenzyme A
-
-
0.45
L-histidine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.3
-
partially purified enzyme
1692
purified native enzyme, pH 7.8, 37C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
-
broad optimum, assay at pH 7.8
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.7
-
pH 7.0: 63% of activity maximum, pH 7.7: activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37 - 50
-
37C: optimum, 50C: less than 10% activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
the amount of enzyme activity is related to starvation and feeding of the fish
Manually annotated by BRENDA team
additional information
the HISAT gene is expressed as two splice variants containing an identical ORF but differing lengths of 5'-UTR. Both variants are expressed exclusively in the fish brain and lens
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 38500, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
the purified enzyme HISAT is an unstable enzyme that is rapidly and irreversibly inactivated during preincubation at 37 C in the absence of acetyl-CoA
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
HISAT is an unstable enzyme that is rapidly and irreversibly inactivated during preincubation at 37 C in the absence of acetyl-CoA
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme 4453fold from brain by ammonium sulfate fractionation, dye-ligand affinity chromatography, gel filtration, and anion exchange chromatography followed by ultrafiltration
partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene hisat, DNA and amino acid sequence determination and analysis
gene hisat, DNA and amino acid sequence determination and analysis, the HISAT gene is expressed as two splice variants containing an identical ORF but differing lengths of 5'-UTR. Both variants are expressed exclusively in the fish brain and lens, recombinant expression in a cell-free translation system, and recombinant expression of the two HISAT splice variant-1 and -2 in Oreochromis niloticus
gene Nat16, DNA and amino acid sequence determination and analysis, recombinant expression in a cell-free translation system