Information on EC 2.3.1.3 - glucosamine N-acetyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.3
-
RECOMMENDED NAME
GeneOntology No.
glucosamine N-acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + D-glucosamine = CoA + N-acetyl-D-glucosamine
show the reaction diagram
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:D-glucosamine N-acetyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9032-94-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
Pigeon
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-
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Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + chitobiose
CoA + N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
show the reaction diagram
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77.5% activity compared to D-glucosamine
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-
?
acetyl-CoA + chitopentaose
?
show the reaction diagram
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4.7% activity compared to D-glucosamine
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-
?
acetyl-CoA + chitotetraose
?
show the reaction diagram
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9.3% activity compared to D-glucosamine
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-
?
acetyl-CoA + chitotriose
?
show the reaction diagram
-
12% activity compared to D-glucosamine
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-
?
acetyl-CoA + D-glucosamine
CoA + N-acetyl-D-glucosamine
show the reaction diagram
acetyl-CoA + galactosamine
CoA + N-acetyl-D-galactosamine
show the reaction diagram
Pigeon
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + D-glucosamine
CoA + N-acetyl-D-glucosamine
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
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-
Cu2+
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-
EDTA
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1 mM EDTA inhibits enzyme by 20%
Fe2+
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-
Fe3+
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-
Zn2+
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-
additional information
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not: Mg2+, Mn2+, Ca2+, mono-, di and tri-phosphates of uridine, cytosine and tymidine
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.24 - 0.26
D-glucosamine
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Pigeon
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-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
230000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 1.3-1.6 M (NH4)2SO4, 0.1 M Bis-Tris pH (5.5–6.5) at 20°C
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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CHAPS and digitonin solubilize 50% of enzyme activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography and Superdex 200 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21-Gold (DE3) cells
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H842A
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inactive