Information on EC 2.3.1.249 - spermidine dicoumaroyl transferase

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The expected taxonomic range for this enzyme is: Arabidopsis thaliana

EC NUMBER
COMMENTARY hide
2.3.1.249
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RECOMMENDED NAME
GeneOntology No.
spermidine dicoumaroyl transferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 4-coumaroyl-CoA + spermidine = 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
show the reaction diagram
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-
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SYSTEMATIC NAME
IUBMB Comments
4-coumaroyl-CoA:spermidine N-(hydroxycinnamoyl)transferase
The enzyme from the plant Arabidopsis thaliana has no activity with sinapoyl-CoA (cf. EC 2.3.1.248, spermidine disinapoyl transferase).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene At2g25150
UniProt
Manually annotated by BRENDA team
gene At2g25150
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 4-coumaroyl-CoA + spermidine
2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 4-coumaroyl-CoA + spermidine
2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0106
4-Coumaroyl-CoA
pH 7.3, 30C, recombinant His6-tagged enzyme
0.0527
spermidine
pH 7.3, 30C, recombinant His6-tagged enzyme
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.4
4-Coumaroyl-CoA
Arabidopsis thaliana
Q8GYW8
pH 7.3, 30C, recombinant His6-tagged enzyme
3.1
spermidine
Arabidopsis thaliana
Q8GYW8
pH 7.3, 30C, recombinant His6-tagged enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His6-tagged enzyme from Escherichia coli strain BL21 Rosetta (DE3) pLysS by nickel affinity chromatography and desalting gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene At2g25150, quantitative real-time RT-PCR expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 Rosetta (DE3) pLysS