Information on EC 2.3.1.248 - spermidine disinapoyl transferase

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The expected taxonomic range for this enzyme is: Arabidopsis thaliana

EC NUMBER
COMMENTARY hide
2.3.1.248
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RECOMMENDED NAME
GeneOntology No.
spermidine disinapoyl transferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 sinapoyl-CoA + spermidine = 2 CoA + N1,N8-bis(sinapoyl)-spermidine
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
sinapoyl-CoA:spermidine N-(hydroxycinnamoyl)transferase
The enzyme from the plant Arabidopsis thaliana has no activity with 4-coumaroyl-CoA (cf. EC 2.3.1.249, spermidine dicoumaroyl transferase).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene At2g23510
UniProt
Manually annotated by BRENDA team
gene At2g23510
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 CoA + N1,N8-bis(sinapoyl)-spermidine
2 sinapoyl-CoA + spermidine
show the reaction diagram
2 sinapoyl-CoA + putrescine
?
show the reaction diagram
2 sinapoyl-CoA + spermidine
2 CoA + N1,N8-bis(sinapoyl)-spermidine
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 sinapoyl-CoA + spermidine
2 CoA + N1,N8-bis(sinapoyl)-spermidine
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0836
CoA
pH 7.3, 30°C, recombinant His6-tagged enzyme
0.0346
N1,N8-bis(sinapoyl)-spermidine
pH 7.3, 30°C, recombinant His6-tagged enzyme
0.2369
putrescine
pH 7.3, 30°C, recombinant His6-tagged enzyme
0.0083
Sinapoyl-CoA
pH 7.3, 30°C, recombinant His6-tagged enzyme
0.0374
spermidine
pH 7.3, 30°C, recombinant His6-tagged enzyme
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
39.6
CoA
Arabidopsis thaliana
O80467
pH 7.3, 30°C, recombinant His6-tagged enzyme
37.8
N1,N8-bis(sinapoyl)-spermidine
Arabidopsis thaliana
O80467
pH 7.3, 30°C, recombinant His6-tagged enzyme
0.3
putrescine
Arabidopsis thaliana
O80467
pH 7.3, 30°C, recombinant His6-tagged enzyme
5.1
Sinapoyl-CoA
Arabidopsis thaliana
O80467
pH 7.3, 30°C, recombinant His6-tagged enzyme
5.6
spermidine
Arabidopsis thaliana
O80467
pH 7.3, 30°C, recombinant His6-tagged enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
recombinant enzyme, reverse reaction
9
recombinant enzyme, forward reaction
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His6-tagged enzyme from Escherichia coli strain BL21 Rosetta (DE3) pLysS by nickel affinity chromatography and desalting gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene At2g23510, quantitative real-time RT-PCR expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 Rosetta (DE3) pLysS
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information