Information on EC 2.3.1.227 - GDP-perosamine N-acetyltransferase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
2.3.1.227
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RECOMMENDED NAME
GeneOntology No.
GDP-perosamine N-acetyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + GDP-4-amino-4,6-dideoxy-alpha-D-mannose = CoA + GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:GDP-4-amino-4,6-dideoxy-alpha-D-mannose N-acetyltransferase
D-Perosamine is one of several dideoxy sugars found in the O-antigen component of the outer membrane lipopolysaccharides of Gram-negative bacteria.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose (i.e. GDP-N-acetyl-alpha-D-perosamine) is a component of the four sugar repeated subunit of the O-antigen of Escherichia coli O157:H7. The enzyme is involved in the biosynthesis of the precursor molecule GDP-N-acetyl-D-perosamine
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + GDP-4-amino-4,6-dideoxy-alpha-D-mannose
CoA + GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + GDP-4-amino-4,6-dideoxy-alpha-D-mannose
CoA + GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose
show the reaction diagram
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GDP-4-amino-4,6-dideoxy-alpha-D-mannose (i.e. GDP-alpha-D-perosamine) is a component of the four sugar repeated subunit of the O-antigen of Escherichia coli O157:H7. The enzyme is involved in the biosynthesis of the precursor molecule GDP-N-acetyl-D-perosamine
GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose i.e. GDP-N-acetyl-alpha-D-perosamine
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?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.8
acetyl-CoA
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pH 8.0, 37°C
0.32
GDP-4-amino-4,6-dideoxy-alpha-D-mannose
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pH 8.0, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
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His-tag PerB protein
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26260
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3 * 26260, His-tag PerB, SDS-PAGE
68000
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His-tag PerB, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
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3 * 26260, His-tag PerB, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65
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His-tag PerB, 20 min, 24% loss of activity
75
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His-tag PerB, 20 min, 90% loss of activity
80
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His-tag PerB, 10 min, complete inactivation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 30 days, His-tag PerB, 5% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-tagged fusion protein
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression as a His-tagged fusion protein in Escherichia coli BL21
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