Information on EC 2.3.1.214 - pelargonidin 3-O-(6-caffeoylglucoside) 5-O-(6-O-malonylglucoside) 4'''-malonyltransferase

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The expected taxonomic range for this enzyme is: Salvia splendens

EC NUMBER
COMMENTARY hide
2.3.1.214
-
RECOMMENDED NAME
GeneOntology No.
pelargonidin 3-O-(6-caffeoylglucoside) 5-O-(6-O-malonylglucoside) 4'''-malonyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
malonyl-CoA + 4'''-demalonylsalvianin = CoA + salvianin
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Anthocyanin biosynthesis
-
-
salvianin biosynthesis
-
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SYSTEMATIC NAME
IUBMB Comments
malonyl-CoA:4'''-demalonylsalvianin 4'''-O-malonyltransferase
Isolated from the plant Salvia splendens (scarlet sage).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzyme belongs to the BAHD family of acyltrasferases, and phylogenetic analysis shows, that it is more related to the acetyl-CoA:benzylalcohol acetyltransferases than to the anthocyanin acyltransferases. Ss5MaT2 contains the BAHD conserved motifs 1 HXXXD and 3 DFGWG, but lacks motif 2 YFGNC
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
malonyl-CoA + 4'''-demalonylsalvianin
CoA + salvianin
show the reaction diagram
-
-
-
?
malonyl-CoA + monodemalonylsalvianin
CoA + salvianin
show the reaction diagram
Ss5MaT2 activity with monodemalonylsalvianin
-
-
?
additional information
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0513
4'''-demalonylsalvianin
pH 8.5, temperature not specified in the publication
0.0069
malonyl-CoA
pH 8.5, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.55
4'''-demalonylsalvianin
Salvia splendens
Q6TXD2
pH 8.5, temperature not specified in the publication
0.55
malonyl-CoA
Salvia splendens
Q6TXD2
pH 8.5, temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10.7
4'''-demalonylsalvianin
Salvia splendens
Q6TXD2
pH 8.5, temperature not specified in the publication
13700
79.7
malonyl-CoA
Salvia splendens
Q6TXD2
pH 8.5, temperature not specified in the publication
76
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.144
purified native enzyme, pH 8.5, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
recently opened, fully pigmented flowers
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43643
1 * 46000, SDS-PAGE, 1 * 43643, sequence calculation
45000
gel filtration
46000
1 * 46000, SDS-PAGE, 1 * 43643, sequence calculation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 46000, SDS-PAGE, 1 * 43643, sequence calculation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native Ss5MaT2 510fold by ammonium sulfate fractionation, a first anion exchange chromatographic step, hydrophobic interaction and ceramic hydroxyapatite chromatography, gel filtration, and a second, different anion exchange chromatograpic step to over 95% purity. Recombinant N-terminally His-tagged SsAT412 from Escherichia coli strain JM109 to homogeneity
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ss5MaT2 or SsAT412, DNA and amino acid sequence determination and analysis, phylogenetic analysis, soluble N-terminally His-tagged SsAT412 expression in Escherichia coli strain JM109