Information on EC 2.3.1.200 - lipoyl amidotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.200
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RECOMMENDED NAME
GeneOntology No.
lipoyl amidotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[glycine cleavage system H]-N6-lipoyl-L-lysine + a [lipoyl-carrier protein] = glycine cleavage system H + a [lipoyl-carrier protein]-N6-lipoyl-L-lysine
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
lipoate salvage II
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SYSTEMATIC NAME
IUBMB Comments
[glycine cleavage system H]-N6-lipoyl-L-lysine:[lipoyl-carrier protein]-N6-L-lysine lipoyltransferase
In the bacterium Listeria monocytogenes the enzyme takes part in a pathway for scavenging of lipoic acid. The enzyme is bound to 2-oxo-acid dehydrogenases such as the pyruvate dehydrogenase complex, where it transfers the lipoyl moiety from lipoyl-[glycine cleavage system H] to the E2 subunits of the complexes.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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the enzyme is essential for lipoic acid synthesis, but has no detectable octanoyltransferase or ligase activity either in vitro or in vivo. The enzyme is required for PdhC modification
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glycine cleavage system H protein + octanoyl-branched chain dehydrogenase E2 subunit-lipoyl domain
octanoyl-glycine cleavage system H protein + branched chain dehydrogenase E2 subunit-lipoyl domain
show the reaction diagram
lipoyl-glycine cleavage system H protein + branched chain dehydrogenase E2 subunit-lipoyl domain
glycine cleavage system H protein + lipoyl-branched chain dehydrogenase E2 subunit-lipoyl domain
show the reaction diagram
octanoyl-glycine cleavage system H protein + pyruvate dehydrogenase subunit E2
glycine cleavage system H protein + pyruvate dehydrogenase subunit E2-octanoyl
show the reaction diagram
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r
additional information
?
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA resin column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain QC143
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expressed in Escherichia coli strain QC244
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C150A
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the mutation results in loss of enzymatic activity and the inability to form an acyl-enzyme intermediate
C150S
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the mutation results in loss of enzymatic activity and the inability to form an acyl-enzyme intermediate