Information on EC 2.3.1.198 - glycerol-3-phosphate 2-O-acyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.198
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RECOMMENDED NAME
GeneOntology No.
glycerol-3-phosphate 2-O-acyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an acyl-CoA + sn-glycerol 3-phosphate = CoA + a 2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Glycerophospholipid metabolism
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SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:sn-glycerol-3-phosphate 2-O-acyltransferase
A membrane-associated enzyme required for suberin or cutin synthesis in plants. Active with a wide range of acyl-CoA substrates (C16:0-C24:0). The enzyme from some sources has much higher activity with omega-oxidized acyl-CoAs. Some enzymes are bifunctional and have an additional phosphatase activity producing sn-2-monoacylglycerols.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
H9XTI6, i.e. isoform Gpat4-A1, H9XTI4 i.e. isoform Gpat4-C1, H9XTI5, i.e. isoform Gpat4-C2
H9XTI6 and H9XTI4 and H9XTI5
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
sn-glycerol 3-phosphate + (9Z)-17-carboxyheptadec-9-enoyl-CoA
CoA + 1-((9Z)-17-carboxyheptadec-9-enoyl)-sn-glycerol 3-phosphate + 2-((9Z)-17-carboxyheptadec-9-enoyl)-sn-glycerol 3-phosphate
show the reaction diagram
(9Z)-17-carboxyheptadec-9-enoyl-CoA = C18:1 DCA-CoA
((9Z)-17-carboxyheptadec-9-enoyl)-sn-glycerol 3-phosphate = C18:1 DCA-LPA, ratio of sn-2:sn-1-labeled products is 5:1 or greater
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sn-glycerol 3-phosphate + 15-carboxypentadecanoyl-CoA
CoA + 1-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate + 2-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate
show the reaction diagram
sn-glycerol 3-phosphate + 15-carboxypentadecanoyl-CoA
CoA + 2-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate
show the reaction diagram
H9XTI6 and H9XTI4 and H9XTI5
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reaction mainly occurs in sn-2 position. Intrinsic phosphatase activity of the enzymes finally leads to formation of 2-(15-carboxypentadecanoyl)-sn-glycerol
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?
sn-glycerol 3-phosphate + palmitoyl-CoA
CoA + 2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
H9XTI6 and H9XTI4 and H9XTI5
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phosphatase activity of the enzymes does not act on non-substituted acyl residues
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?
additional information
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H9XTI6 and H9XTI4 and H9XTI5
all three Gpat4 isoforms exhibit sn-2 acyltransferase and phosphatase activities with dicarboxylic acid-CoA as acyl donor. When non-substituted acyl-CoA is used as acyl donor, the rate of acylation is considerably lower and phosphatase activity is not manifested
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
activity assay at; activity assay at; activity assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
activity assay at room temperature; activity assay at room temperature; activity assay at room temperature
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
modeling data indicate this localisation; modeling data indicate this localisation; modeling data indicate this localisation
Manually annotated by BRENDA team
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned in yeast microsomes and wheat germ cells