Information on EC 2.3.1.197 - dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose 3-N-acetyltransferase

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The expected taxonomic range for this enzyme is: Aneurinibacillus thermoaerophilus

EC NUMBER
COMMENTARY hide
2.3.1.197
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RECOMMENDED NAME
GeneOntology No.
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose 3-N-acetyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose = CoA + dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactopyranose
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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dTDP-3-acetamido-alpha-D-fucose biosynthesis
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Polyketide sugar unit biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose 3-N-acetyltransferase
The product, dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose, is a component of the glycan chain of the crystalline bacterial cell surface layer protein (S-layer glycoprotein) of Aneurinibacillus thermoaerophilus.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
CoA + dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactopyranose
show the reaction diagram
the enzyme is involved in the biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose. The reverse reaction cannot be detected
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?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.061
acetyl-CoA
pH 7.4, 37°C
0.0667
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
pH 7.4, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.1
acetyl-CoA
Aneurinibacillus thermoaerophilus
Q6T1W7
pH 7.4, 37°C
2.3
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
Aneurinibacillus thermoaerophilus
Q6T1W7
pH 7.4, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
50.8
acetyl-CoA
Aneurinibacillus thermoaerophilus
Q6T1W7
pH 7.4, 37°C
29
3.4
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
Aneurinibacillus thermoaerophilus
Q6T1W7
pH 7.4, 37°C
10341
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
to increase the stability, the enzyme is concentrated by ultrafiltration before storage at 4°C or -20°C and supplemented with 50% glycerol
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, the concentrated enzyme preparations are stable at 4°C for several months with only a marginal decrease of enzyme activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli