Information on EC 2.3.1.195 - (Z)-3-hexen-1-ol acetyltransferase

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The expected taxonomic range for this enzyme is: Arabidopsis thaliana

EC NUMBER
COMMENTARY hide
2.3.1.195
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RECOMMENDED NAME
GeneOntology No.
(Z)-3-hexen-1-ol acetyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + (3Z)-hex-3-en-1-ol = CoA + (3Z)-hex-3-en-1-yl acetate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
traumatin and (Z)-3-hexen-1-yl acetate biosynthesis
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alpha-Linolenic acid metabolism
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:(3Z)-hex-3-en-ol acetyltransferase
The enzyme is resonsible for the production of (3Z)-hex-3-en-1-yl acetate, the major volatile compound released upon mechanical wounding of the leaves of Arabidopsis thaliana [1].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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RNAi transgenic plants exhibiting a severe reduction in At3g03480 transcript levels emitt no (Z)-3-hexen-1-yl acetate
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + (3Z)-hex-3-en-ol
(3Z)-hex-3-en-1-yl acetate + CoA
show the reaction diagram
acetyl-CoA + (3Z)-hex-3-en-ol
(Z)-3-hexen-1-yl acetate + coenzyme A
show the reaction diagram
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acetyl-CoA + 1-octanol
octanyl acetate + CoA
show the reaction diagram
the enzyme has the ability to accept several medium-chain-length aliphatic and benzyl-derived alcohols, but has highest catalytic efficiency with (Z)-3-hexen-1-ol. The catalytic efficiency with 1-octanol is 0.2% of the catalytic efficiency with (Z)-3-hexen-1-ol
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?
additional information
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + (3Z)-hex-3-en-ol
(3Z)-hex-3-en-1-yl acetate + CoA
show the reaction diagram
Q9SRQ2
the major volatile released upon mechanical wounding of the leaves is (Z)-3-hexen-1-yl acetate
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?
acetyl-CoA + (3Z)-hex-3-en-ol
(Z)-3-hexen-1-yl acetate + coenzyme A
show the reaction diagram
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
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5 mM, strong inhibition
Co2+
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5 mM, strong inhibition
Cu2+
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5 mM, strong inhibition
Mg2+
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5 mM, strong inhibition
Zn2+
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5 mM, strong inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.126 - 0.251
(3Z)-hex-3-en-ol
0.174
1-Octanol
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pH 7.7, 25C, recombinant His6-tagged enzyme
0.0105 - 0.0385
acetyl-CoA
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11.3
(3Z)-hex-3-en-ol
Arabidopsis thaliana
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pH 7.7, 25C, recombinant His6-tagged enzyme
0.125
1-Octanol
Arabidopsis thaliana
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pH 7.7, 25C, recombinant His6-tagged enzyme
23
acetyl-CoA
Arabidopsis thaliana
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pH 7.7, 25C, recombinant His6-tagged enzyme
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
45
(3Z)-hex-3-en-ol
Arabidopsis thaliana
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pH 7.7, 25C, recombinant His6-tagged enzyme
15025
0.072
1-Octanol
Arabidopsis thaliana
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pH 7.7, 25C, recombinant His6-tagged enzyme
1228
597
acetyl-CoA
Arabidopsis thaliana
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pH 7.7, 25C, recombinant His6-tagged enzyme
29
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3 - 9
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pH 6.3: 89% of maximal activity, pH 9.0: about 45% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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enzyme activity in the leaves following wounding increases over time, but this response lagges significantly behind (Z)-3-hexen-1-yl acetate release. A maximum specific activity is reached 12 h after the initial wounding event. CHAT enzyme activity declines by 35% over the course of the next 12 h
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49000
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native plant protein, gel filtration
50290
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calculated from sequence
51000
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His-tagged protein, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 50290, calculated from sequence
monomer
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1 * 50400, native plant protein, calculated from sequence
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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30 min, stable
37
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30 min, 13% loss of activity
50
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30 min, complete loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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expression in Escherichia coli, the coding region of At3g03480 is cloned into a vector that introduces an N-terminal 6His tag
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