Information on EC 2.3.1.189 - mycothiol synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.189
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RECOMMENDED NAME
GeneOntology No.
mycothiol synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
desacetylmycothiol + acetyl-CoA = CoA + mycothiol
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mycothiol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:desacetylmycothiol O-acetyltransferase
This enzyme catalyses the last step in the biosynthesis of mycothiol, the major thiol in most actinomycetes, including Mycobacterium [1]. The enzyme is a member of a large family of GCN5-related N-acetyltransferases (GNATs) [2]. The enzyme has been purified from Mycobacterium tuberculosis H37Rv. Acetyl-CoA is the preferred CoA thioester but propionyl-CoA is also a substrate [3].
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
desacetylmycothiol + acetyl-CoA
mycothiol + coenzyme A
show the reaction diagram
desacetylmycothiol + acetyl-CoA
mycothiol + coenzyme-A
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
desacetylmycothiol + acetyl-CoA
mycothiol + coenzyme A
show the reaction diagram
desacetylmycothiol + acetyl-CoA
mycothiol + coenzyme-A
show the reaction diagram
PDB
SCOP
CATH
ORGANISM
UNIPROT
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
vapor diffusion under oil method
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vapor diffusion under oil, 1.8 A resolution structure of MshD in complex with the disulfide of CoA and desacetylmycothiol
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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the Rv0819 gene encoding MshD (residues 1–315) is amplified from Mycobacterium tuberculosis genomic DNA using PCR and cloned into the pET28a(+) plasmid. This vector construction adds six histidine residues and a thrombin cleavage sequence to the N-terminus to facilitate protein purification. Expression in Escherichia coli stain BL21(DE3)pLysS
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Show AA Sequence (1308 entries)
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