Information on EC 2.3.1.188 - omega-hydroxypalmitate O-feruloyl transferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.188
-
RECOMMENDED NAME
GeneOntology No.
omega-hydroxypalmitate O-feruloyl transferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
feruloyl-CoA + 16-hydroxypalmitate = CoA + 16-feruloyloxypalmitate
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
esterified suberin biosynthesis
-
-
Cutin, suberine and wax biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
feruloyl-CoA:16-hydroxypalmitate feruloyltransferase
p-Coumaroyl-CoA and sinapoyl-CoA also act as substrates. The enzyme is widely distributed in roots of higher plants.
CAS REGISTRY NUMBER
COMMENTARY hide
156859-07-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
feruloyl-CoA + 1-dodecanol
CoA + (E)-dodecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
show the reaction diagram
feruloyl-CoA + 1-hexadecanol
CoA + (E)-hexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
show the reaction diagram
feruloyl-CoA + 1-pentadecanol
CoA + (E)-pentadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
show the reaction diagram
feruloyl-CoA + 1-tetradecanol
CoA + (E)-tetradecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
show the reaction diagram
feruloyl-CoA + 15-hydroxypentadecanoic acid
CoA + (E)-15-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)pentadecanoic acid
show the reaction diagram
feruloyl-CoA + 16-hydroxypalmitic acid
CoA + (E)-16-(3-(4-hydroxy-3-methoxyphenyl)acryloyloxy)hexadecanoic acid
show the reaction diagram
feruloyl-CoA + hexadecane-1,16-diol
CoA + (E)-16-hydroxyhexadecyl 3-(4-hydroxy-3-methoxyphenyl)acrylate
show the reaction diagram
p-coumaroyl-CoA + 16-hydroxypalmitic acid
CoA + (E)-16-(3-(4-hydroxyphenyl)acryloyloxy)hexadecanoic acid
show the reaction diagram
sinapoyl-CoA + 16-hydroxypalmitic acid
CoA + (E)-16-(3-(4-hydroxy-3,5-dimethoxyphenyl)acryloyloxy)hexadecanoic acid
show the reaction diagram
additional information
?
-
-
no substrate: 18-hydroxy-cis-9-octadecenoic acid
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Tris-HCl
-
60% of maximal activity in Tris-HCl compared with potassium phosphate buffer
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002 - 0.005
1-Dodecanol
0.01
1-hexadecanol
-
pH 7.0, 30C
0.0095
1-pentadecanol
-
pH 7.0, 30C
0.001 - 0.004
1-Tetradecanol
0.0112 - 0.0168
15-hydroxypentadecanoic acid
0.0003 - 0.001
16-hydroxypalmitic acid
0.005 - 0.015
Feruloyl-CoA
0.0007 - 0.0017
hexadecan-1,16-diol
0.01 - 0.0143
p-Coumaroyl-CoA
0.0033 - 0.0071
Sinapoyl-CoA
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000048
-
substrate 1-tetradecanol, pH 7.0
0.000075
-
substrate 16-hydroxypalmitate, pH 7.0
0.00083
-
substrate 1-tetradecanol, pH 7.0
0.001
-
substrate 16-hydroxypalmitate, pH 7.0
0.0014
-
substrate 1-tetradecanol, pH 7.0
0.0024
-
substrate 16-hydroxypalmitate, pH 7.0
0.0208
-
substrate 1-tetradecanol, pH 7.0
0.0288
-
substrate 16-hydroxypalmitate, pH 7.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
potassium phosphate buffer, 60% of maximal activity in Tris-HCl
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
half-maximal activity
7.5
-
half-maximal activity
10
-
5% of maximal acitvity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
wound-healing tuber disc
Manually annotated by BRENDA team
additional information
-
no activity in stem or leaf
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 55000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE