Information on EC 2.3.1.187 - acetyl-S-ACP:malonate ACP transferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.187
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RECOMMENDED NAME
GeneOntology No.
acetyl-S-ACP:malonate ACP transferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an acetyl-[acyl-carrier protein] + malonate = a malonyl-[acyl-carrier protein] + acetate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
malonate degradation I (biotin-independent)
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malonate degradation II (biotin-dependent)
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SYSTEMATIC NAME
IUBMB Comments
acetyl-[acyl-carrier-protein]:malonate S-[acyl-carrier-protein]transferase
This is the first step in the catalysis of malonate decarboxylation and involves the exchange of an acetyl thioester residue bound to the activated acyl-carrier protein (ACP) subunit of the malonate decarboxylase complex for a malonyl thioester residue [2]. This enzyme forms the alpha subunit of the multienzyme complexes biotin-independent malonate decarboxylase (EC 4.1.1.88) and biotin-dependent malonate decarboxylase (EC 4.1.1.89). The enzyme can also use acetyl-CoA as a substrate but more slowly [4].
CAS REGISTRY NUMBER
COMMENTARY hide
80700-20-5
multienzyme complex malonate decarboxylase
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene madA, encoded in the malonate decarboxylase operon mad
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Manually annotated by BRENDA team
alpha subunit of malonate decarboxylase complex
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
malonate + acetyl-[acyl-carrier protein]
acetate + malonyl-[acyl-carrier protein]
show the reaction diagram
malonyl-CoA + dephospho-CoA
CoA + malonyl-dephospho-CoA
show the reaction diagram
methylmalonyl-CoA + dephospho-CoA
CoA + methylmalonyl-dephospho-CoA
show the reaction diagram
additional information
?
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no substrate: acetyl-CoA
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
malonate + acetyl-[acyl-carrier protein]
acetate + malonyl-[acyl-carrier protein]
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-ethylmaleimide
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preincubation with malonyl-CoA or methylmalonyl-CoA protects
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.016 - 0.05
malonyl-CoA
0.01 - 0.016
methylmalonyl-CoA
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.6
alpha subunit of malonate decarboxylase complex
2.4
native enzyme complex
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60000
x * 60000, SDS-PAGE
60800
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x * 60800, isolated acetyl-S-ACP:malonate ACP transferase C-terminal alpha subunit of the malonate decarboxylase complex, SDS-PAGE
61200
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x * 61200, isolated acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase moiety of the enzyme complex, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
isolated soluble native transferase component of the malonate decarboxylase
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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expression in Escherichia coli, coexpression with GroEL/ES
gene mdcA, encoded in the malonate decarboxylase operon mdc, genetic organization
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